A Photosensitizer-Loaded Polydopamine Nanomedicine Agent for Synergistic Photodynamic and Photothermal Therapy
Abstract
:1. Introduction
2. Results
2.1. Synthesis and Characterization of PDA-ZnPc+ Nps
2.2. ROS and Photothermal Effect of PDA-ZnPc+ Nps
2.3. In Vitro Cytotoxicity of PDA-ZnPc+ Nps
2.4. Apoptosis
2.5. Fluorescence Imaging
2.6. In Vivo Combinational PTT/PDT
2.7. In Vivo Safety Analysis of PDA-ZnPc+ Nps
3. Discussion
4. Materials and Methods
4.1. Chemicals and Cell Lines
4.2. Preparation of ZnPc(4TAP)12+ (ZnPc+)
4.3. Synthesis of PDA
4.4. Synthesis of PDA-ZnPc(4TAP)12+ Nanoparticles (PDA-ZnPc+ Nps)
4.5. Characterization of PDA-ZnPc+ Nps
4.6. ROS and Photothermal Effect of PDA-ZnPc+ Nps
4.7. In Vitro PDT, PTT, and Synergistic PDT/PTT
4.8. Apoptosis Detection
4.9. Fluorescence Imaging
4.10. Establishment of MCF-7 Tumor-Bearing Mouse Model
4.11. In Vivo Combinational PTT/PDT
4.12. H&E Staining Assay
4.13. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Yan, S.; Dong, L.; Hu, Z.; Zhang, Y.; Xu, W.; Xing, J.; Zhang, J. A Photosensitizer-Loaded Polydopamine Nanomedicine Agent for Synergistic Photodynamic and Photothermal Therapy. Molecules 2023, 28, 5874. https://doi.org/10.3390/molecules28155874
Yan S, Dong L, Hu Z, Zhang Y, Xu W, Xing J, Zhang J. A Photosensitizer-Loaded Polydopamine Nanomedicine Agent for Synergistic Photodynamic and Photothermal Therapy. Molecules. 2023; 28(15):5874. https://doi.org/10.3390/molecules28155874
Chicago/Turabian StyleYan, Shufeng, Luying Dong, Ziyun Hu, Yucheng Zhang, Wei Xu, Jianhong Xing, and Juncheng Zhang. 2023. "A Photosensitizer-Loaded Polydopamine Nanomedicine Agent for Synergistic Photodynamic and Photothermal Therapy" Molecules 28, no. 15: 5874. https://doi.org/10.3390/molecules28155874