Icaritin Promotes Myelination by Simultaneously Enhancing the Proliferation and Differentiation of Oligodendrocyte Precursor Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. Icaritin Promotes Oligodendrocyte Precursor Cell Viability and Differentiation
2.2. Icaritin Promotes Oligodendrocyte Precursor Cell Proliferation under Pathological Conditions In Vitro
2.3. Icaritin Improved Spontaneous Activity and Remyelination In Vivo
2.4. Potential Mechanisms of Icaritin
3. Material and Methods
3.1. Materials
3.2. Cell Line
3.3. Effects of TCM on Oligodendrocyte Precursor Cells Viability and Differentiation
3.3.1. Effects of Herbs on Cell Viability of Oligodendrocyte Precursor Cells
3.3.2. Effects of Herbal Monomers on Differentiation
3.3.3. Effects of Herbal Monomers on Cell Viability under Pathological Conditions In Vitro
3.3.4. Effects of Icaritin on Differentiation under Pathological Models In Vitro
3.4. Animal Study
3.4.1. Open Filed and Y-Maze Tests
3.4.2. Expressions of MBP in Hippocampus
3.5. Mechanisms
3.5.1. Effects of Icaritin on Ach, ChAT, and AchE
3.5.2. Effects of Icaritin on Myelin-Related Genes
3.5.3. Neuroprotective Effects of Icaritin against Pathological Damage of Myelin Related Pathway
3.6. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Group | Concentration | A2B5+ OPCs (%) |
---|---|---|
CTRL | / | 61.53 ± 1.89 |
Catalpol | 5 μM | 70.83 ± 1.60 ** |
Geniposide | 69.20 ± 3.66 * | |
Emodin | 62.44 ± 3.79 | |
Longistyline A | 59.35 ± 1.91 | |
Curcumin | 66.80 ± 2.91 | |
Resveratrol | 68.50 ± 3.24 * | |
Genipin | 65.36 ± 3.78 | |
Icaritin | 75.34 ± 2.97 *** | |
Icariin | 66.27 ± 3.53 | |
Quercetin | 62.48 ± 2.55 | |
Apigenin | 61.95 ± 5.80 | |
Loganin | 62.23 ± 2.65 | |
Morroniside | 59.01 ± 1.49 |
Group | Concentration | MBP+ OLs (%) |
---|---|---|
+T3 | 60 ng/mL | 19.03 ± 1.50 ** |
−T3 | / | 11.37 ± 1.84 |
Catalpol | 5 μM | 15.47 ± 2.38 |
Geniposide | 14.21 ± 3.17 | |
Emodin | 6.05 ± 2.39 | |
Cajanine | 10.36 ± 4.52 | |
Longistyline A | 5.19 ± 3.85 | |
Curcumin | 8.92 ± 3.17 | |
Resveratrol | 14.20 ± 3.56 | |
Genipin | 12.59 ± 2.44 | |
Icaritin | 17.00 ± 1.61 * | |
Icariin | 17.86 ± 1.40 ** | |
Quercetin | 9.77 ± 1.58 | |
Apigenin | 10.83 ± 3.69 | |
Loganin | 7.32 ± 2.11 | |
Morroniside | 11.46 ± 2.87 |
Primer | 5′ to 3′ |
---|---|
MBP | F: GCTTCTTTAGCGGTGACAGGG |
R: TGGAGGTGGTGTTCGAGGTG | |
SOX10 | F: AGCCCAGGTGAAGACAGAGA |
R: CCCCTCTAAGGTCGGGATAG | |
NGN3 | F: CTGCGCATAGCGGACCACAGCTTC |
R: CTTCACAAGAAGTCTGAGAACACCAG | |
NKX2.2 | F: CTCCAATACTCCCTGCACGG |
R: GATTGTGCTCCCAGGCCTG | |
PDGFaa | F: ACCAGACCCAGACATGGCC |
R: AAGACGGCACAGGTCACCAC | |
Olig2 | F: TGGCTTCAAGTCATCTTCCTCC |
R: TCGCTCACCAGTCGCTTCAT |
Reagent | Volume (μL) |
---|---|
SYBR Premix EX Taq II (Tli RNaseH Plus) | 12.5 |
PCR Forward Primer (10 μM) | 1 |
PCR Reverse Primer (10 μM) | 1 |
dDNA | 2 |
dH2O | 8.5 |
Total | 25 |
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Yang, F.; Wen, H.; Ma, S.; Chang, Q.; Pan, R.; Liu, X.; Liao, Y. Icaritin Promotes Myelination by Simultaneously Enhancing the Proliferation and Differentiation of Oligodendrocyte Precursor Cells. Molecules 2023, 28, 5837. https://doi.org/10.3390/molecules28155837
Yang F, Wen H, Ma S, Chang Q, Pan R, Liu X, Liao Y. Icaritin Promotes Myelination by Simultaneously Enhancing the Proliferation and Differentiation of Oligodendrocyte Precursor Cells. Molecules. 2023; 28(15):5837. https://doi.org/10.3390/molecules28155837
Chicago/Turabian StyleYang, Feifei, Han Wen, Siqi Ma, Qi Chang, Ruile Pan, Xinmin Liu, and Yonghong Liao. 2023. "Icaritin Promotes Myelination by Simultaneously Enhancing the Proliferation and Differentiation of Oligodendrocyte Precursor Cells" Molecules 28, no. 15: 5837. https://doi.org/10.3390/molecules28155837