Photocatalytic Degradation of Methylene Blue and Anticancer Response of In₂O₃/RGO Nanocomposites Prepared by a Microwave-Assisted Hydrothermal Synthesis Process

The incorporation of graphene with metal oxide has been widely explored in various fields, including energy storage devices, optical applications, biomedical applications, and water remediation. This research aimed to assess the impact of reduced graphene oxide (RGO) doping on the photocatalytic and anticancer properties of In₂O₃ nanoparticles. Pure and In₂O₃/RGO nanocomposites were effectively synthesized using the single-step microwave hydrothermal process. XRD, TEM, SEM, EDX, XPS, Raman, UV–Vis, and PL spectroscopy were carefully utilized to characterize the prepared samples. XRD data showed that synthesized In₂O₃ nanoparticles had high crystallinity with a decreased crystal size after RGO doping. TEM and SEM images revealed that the In₂O₃ NPs were spherical and uniformly embedded onto the surface of RGO sheets. Elemental analysis of In₂O₃/RGO NC confirmed the presence of In, O, and C without impurities. Raman analysis indicated the successful fabrication of In₂O₃ onto the RGO surface. Uv–Vis analysis showed that the band gap energy was changed with RGO addition. Raman spectra confirmed that In₂O₃ nanoparticles were successfully anchored onto the RGO sheet. PL results indicated that the prepared In₂O₃/RGO NCs can be applied to enhance photocatalytic activity and biomedical applications. In the degradation experiment, In₂O₃/RGO NCs exhibited superior photocatalytic activity compared to that of pure In₂O₃. The degradation efficiency of In₂O₃/RGO NCs for MB dye was up to 90%. Biological data revealed that the cytotoxicity effect of In₂O₃/RGO NCs was higher than In₂O₃ NPs in human colorectal (HCT116) and liver (HepG2) cancer cells. Importantly, the In₂O₃/RGO NCs exhibited better biocompatibility against human normal peripheral blood mononuclear cells (PBMCs). All the results suggest that RGO addition improves the photocatalytic and anticancer activity of In₂O₃ NPs. This study highlights the potential of In₂O₃/RGO NCs as an efficient photocatalyst and therapeutic material for water remediation and biomedicine.