A Comparative Evaluation of the Photosensitizing Efficiency of Porphyrins, Chlorins and Isobacteriochlorins toward Melanoma Cancer Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. Photosensitizers: Synthesis and Characterization
2.2. PDT Assays
3. Materials and Methods
3.1. Generalities
3.2. Synthesis of the Photosensitizers (PS)
3.3. Cell Culture
3.4. Cell Viability Assay
3.5. Statistical Analysis
3.6. Fluorescence Studies
3.7. Stability Studies
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Compound | Soret Band λmax (nm) | Q Bands λmax (nm) | a λemission (nm) | b ΦF | c τ1 (ns) | c τ2 (ns) | c τT (μs) | d ΦΔ | |||
---|---|---|---|---|---|---|---|---|---|---|---|
Por1 | 410 | 504 | 539 | 582 | 638 | 638/698 | 0.01 | 10.0 (100) | - | 0.98 | 0.55 |
Chl1 | 405 | 505 | 540 | 595 | 653 | 654 | 0.15 | 6.02 (100) | - | 0.51 | 0.42 |
Iso1 | 380 | 510 | 548 | 586 | 648 | 600/654 | 0.13 | 5.40 (97) | 1.20 (3.0) | 0.50 | 0.31 |
Por2 | 412 | 506 | 538 | 582 | 635 | 638/700 | 0.06 | 11.1 (100) | - | 0.76 | 0.65 |
Chl2 | 406 | 504 | 532 | 595 | 649 | 649 | 0.16 | 6.90 (100) | - | 0.74 | 0.81 |
Iso2 | 386 | 506 | 540 | 580 | 660 | 638/700 | 0.21 | 6.39 (23) | 1.58 (77) | 0.63 | 0.35 |
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Castro, K.A.D.F.; Moura, N.M.M.; Simões, M.M.Q.; Mesquita, M.M.Q.; Ramos, L.C.B.; Biazzotto, J.C.; Cavaleiro, J.A.S.; Faustino, M.A.F.; Neves, M.G.P.M.S.; da Silva, R.S. A Comparative Evaluation of the Photosensitizing Efficiency of Porphyrins, Chlorins and Isobacteriochlorins toward Melanoma Cancer Cells. Molecules 2023, 28, 4716. https://doi.org/10.3390/molecules28124716
Castro KADF, Moura NMM, Simões MMQ, Mesquita MMQ, Ramos LCB, Biazzotto JC, Cavaleiro JAS, Faustino MAF, Neves MGPMS, da Silva RS. A Comparative Evaluation of the Photosensitizing Efficiency of Porphyrins, Chlorins and Isobacteriochlorins toward Melanoma Cancer Cells. Molecules. 2023; 28(12):4716. https://doi.org/10.3390/molecules28124716
Chicago/Turabian StyleCastro, Kelly A. D. F., Nuno M. M. Moura, Mário M. Q. Simões, Mariana M. Q. Mesquita, Loyanne C. B. Ramos, Juliana C. Biazzotto, José A. S. Cavaleiro, M. Amparo F. Faustino, Maria Graça P. M. S. Neves, and Roberto S. da Silva. 2023. "A Comparative Evaluation of the Photosensitizing Efficiency of Porphyrins, Chlorins and Isobacteriochlorins toward Melanoma Cancer Cells" Molecules 28, no. 12: 4716. https://doi.org/10.3390/molecules28124716