Functional Study and Efficient Catalytic Element Mining of CYP76AHs in Salvia Plants
Abstract
:1. Introduction
2. Results
2.1. Chemical Constituents of Salvia
2.2. Phylogenetic Analysis of Candidate CYP76AH Subfamily Genes
2.3. Biochemical Characterization of CYP76Ahs
2.4. Correlation Analysis of CYP76AH Protein Structure and Activity
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Chemicals
4.2. Chemical Constituents of Salvia
4.3. Identification of CYP76AH Genes
4.4. Gene Diversity of CYP76AH Genes
4.5. Heterologous Expression of CYP76AH in Yeast and In Vitro Activity Assays
4.6. Chemical Analysis of the Enzyme Reaction
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Amino Acid Similarity (%) | Sequence Differences | |||||
---|---|---|---|---|---|---|
SmCYP76AH1 | SmCYP76AH3 | SpCYP76AH22 | SpCYP76AH24 | SmCYP76AH1 | SmCYP76AH3 | |
SaeCYP76AH3 | 99.60 | 79.60 | 78.26 | 77.73 | S3Y, K329R | |
ScaCYP76AH3 | 95.91 | 77.00 | 75.76 | 75.24 | S3Y, KS494NPRIRNTTHYRARASTWNRS | |
SdaCYP76AH1 | 98.99 | 78.99 | 77.67 | 77.14 | ||
SdaCYP76AH3 | 80.00 | 99.39 | 86.93 | 86.85 | S5P, Q161E, K320Q | |
SflCYP76AH3 | 79.39 | 96.76 | 87.33 | 86.85 | S5P, F12L, S14T, S21F, S22F, R157K, Q161K, A258T, D261N, N277Q, N279S, V315I, A322G, A394G, D466N, R488K | |
ShoCYP76AH1 | 99.39 | 79.60 | 78.26 | 77.53 | F4S, T18I, K384M | |
ShoCYP76AH3 | 79.80 | 99.39 | 86.73 | 86.65 | S3N, S5P, K320E | |
ShyCYP76AH3 | 99.80 | 79.80 | 78.46 | 77.93 | S3I | |
SjaCYP76AH1 | 98.99 | 79.60 | 77.43 | 76.49 | D2E, F4S, I13T, T18I, K156R | |
SjaCYP76AH3 | 80.00 | 98.58 | 86.93 | 86.65 | S5P, H156D, R157K, D319H, K320Q, R345S, Q473G | |
SprCYP76AH1 | 99.19 | 79.39 | 78.06 | 77.53 | D2E, F4S, N279S | |
SprCYP76AH3-01 | 80.30 | 96.76 | 87.52 | 87.65 | S3Y, S5P, S14T, S21F, S22F, R157K, Q161K, D261N, N277Q, N279S, A322G, D406E, A407S, Q409E, D466N, R488K | |
SprCYP76AH3-02 | 76.67 | 85.49 | 83.88 | 83.23 | ||
SseCYP76AH24 | 79.39 | 79.60 | 86.93 | 86.25 |
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Zhao, Z.; Yang, D.; Guo, J.; Liu, X.; Li, Q.; Su, P.; Wang, J.; Ma, Y.; Huang, L. Functional Study and Efficient Catalytic Element Mining of CYP76AHs in Salvia Plants. Molecules 2023, 28, 4711. https://doi.org/10.3390/molecules28124711
Zhao Z, Yang D, Guo J, Liu X, Li Q, Su P, Wang J, Ma Y, Huang L. Functional Study and Efficient Catalytic Element Mining of CYP76AHs in Salvia Plants. Molecules. 2023; 28(12):4711. https://doi.org/10.3390/molecules28124711
Chicago/Turabian StyleZhao, Zhenyu, Dongfeng Yang, Juan Guo, Xiuyu Liu, Qishuang Li, Ping Su, Jian Wang, Ying Ma, and Luqi Huang. 2023. "Functional Study and Efficient Catalytic Element Mining of CYP76AHs in Salvia Plants" Molecules 28, no. 12: 4711. https://doi.org/10.3390/molecules28124711