Benzoylaconitine Alleviates Progression of Psoriasis via Suppressing STAT3 Phosphorylation in Keratinocytes
Abstract
:1. Introduction
2. Results
2.1. BAC Restrains the Abnormal Proliferation but Not the Survival of HaCaT Cells
2.2. BAC Suppressed the Production of Inflammatory Cytokines in TNF-α/LPS-Induced HaCaT Cells
2.3. BAC Attenuated IMQ-Induced Murine Psoriasis-like Skin Inflammation
2.4. BAC Inhibited the Th17 Cells Accumulation in Model Mice
2.5. BAC Reduced the Release of Inflammatory Cytokines in IMQ-Induced Mouse Skin
2.6. Safety of BAC Administration
2.7. BAC-Regulated STAT3 Pathways in Keratinocytes
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. Detection of Cell Viability
4.3. RNA Extraction and RT-qPCR
4.4. Measurement of Inflammatory Cytokines by ELISA
4.5. IMQ-Induced Psoriasis-like Skin Inflammation in Mice
4.6. Skin Cell Preparation and Flow Cytometry
4.7. Western Blotting
4.8. Skin Histopathological Analysis
4.9. Drug Safety Analyses
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Li, Y.; Guo, D.; Wang, Q.; Li, A.; Yin, S.; Li, S.; Li, Y.; Wang, B.; Guo, T.; Feng, S. Benzoylaconitine Alleviates Progression of Psoriasis via Suppressing STAT3 Phosphorylation in Keratinocytes. Molecules 2023, 28, 4473. https://doi.org/10.3390/molecules28114473
Li Y, Guo D, Wang Q, Li A, Yin S, Li S, Li Y, Wang B, Guo T, Feng S. Benzoylaconitine Alleviates Progression of Psoriasis via Suppressing STAT3 Phosphorylation in Keratinocytes. Molecules. 2023; 28(11):4473. https://doi.org/10.3390/molecules28114473
Chicago/Turabian StyleLi, Yuanbo, Dandan Guo, Qianqian Wang, Aifang Li, Sugai Yin, Shuxuan Li, Yalan Li, Baiyan Wang, Tao Guo, and Shuying Feng. 2023. "Benzoylaconitine Alleviates Progression of Psoriasis via Suppressing STAT3 Phosphorylation in Keratinocytes" Molecules 28, no. 11: 4473. https://doi.org/10.3390/molecules28114473