Next Article in Journal
Stabilization of Natural Pigments in Ethanolic Solutions for Food Applications: The Case Study of Chlorella vulgaris
Previous Article in Journal
Tunable Aryl Alkyl Ionic Liquid Supported Synthesis of Platinum Nanoparticles and Their Catalytic Activity in the Hydrogen Evolution Reaction and in Hydrosilylation
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 28
Issue 1
10.3390/molecules28010401
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Aluminium(III) Oxide—The Silent Killer of Bacteria

by
Mateusz Schabikowski
1,*,
Paweł Kowalczyk
2,*,
Agnieszka Karczmarska
1,
Barbara Gawdzik
3,
Aleksandra Wypych
4,
Karol Kramkowski
5,
Karol Wrzosek
6 and
Łukasz Laskowski
1
1
Institute of Nuclear Physics Polish Academy of Sciences, 31-342 Kraków, Poland
2
Department of Animal Nutrition, The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, 05-110 Jabłonna, Poland
3
Institute of Chemistry, Jan Kochanowski University, 25-406 Kielce, Poland
4
Centre for Modern Interdisciplinary Technologies, Nicolaus Copernicus University in Toruń, 87-100 Toruń, Poland
5
Department of Physical Chemistry, Medical University of Bialystok, 15-089 Białystok, Poland
6
Department of Heart Diseases, The Medical Center of Postgraduate Education, 01-813 Warszawa, Poland
*
Authors to whom correspondence should be addressed.
Molecules 2023, 28(1), 401; https://doi.org/10.3390/molecules28010401
Submission received: 8 December 2022 / Revised: 23 December 2022 / Accepted: 28 December 2022 / Published: 3 January 2023
(This article belongs to the Section Materials Chemistry)
Browse Figures
Review Reports Versions Notes

Abstract

:
In this article, we describe the antimicrobial properties of pristine anodised aluminium oxide matrices—the material many consider biologically inert. During a typical anodisation process, chromium and chlorine compounds are used for electropolishing and the removal of the first-step aluminium oxide. Matrices without the use of those harmful compounds were also fabricated and tested for comparison. The antibacterial tests were conducted on four strains of Escherichia coli: K12, R2, R3 and R4. The properties of the matrices were also compared to the three types of antibiotics: ciprofloxacin, bleomycin and cloxacillin using the Minimal Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) tests. Moreover, DNA was isolated from the analysed bacteria which was additionally digested with formamidopyrimidine-DNA glycosylase (Fpg) protein from the group of repair glycosases. These enzymes are markers of modified oxidised bases in nucleic acids produced during oxidative stress in cells. Preliminary cellular studies, MIC and MBC tests and digestion with Fpg protein after modification of bacterial DNA suggest that these compounds may have greater potential as antibacterial agents than the aforementioned antibiotics. The described composites are highly specific for the analysed model Escherichia coli strains and may be used in the future as new substitutes for commonly used antibiotics in clinical and nosocomial infections in the progressing pandemic era. The results show much stronger antibacterial properties of the functionalised membranes on the action of bacterial membranes in comparison to the antibiotics in the Fpg digestion experiment. This is most likely due to the strong induction of oxidative stress in the cell through the breakdown of the analysed bacterial DNA.

1. Introduction

Anodised aluminium oxide (AAO) has been well known for many years [1,2]. It is a self-organised, highly porous material with regularly ordered pores and, consequently, a high specific surface area [3,4]. Self-organised nanoporous materials, in general, have great potential to be used in a broad range of applications [5] with functionalisation [2,6,7] or additional coatings [8] giving rise to new possibilities. One of the impressive applications of such nanocomposites is the new-generation biocidal materials which may be applied as disinfecting surfaces in both public spaces and medical facilities [9,10,11,12]. Such action in times of the SARS-CoV-2 pandemic and hospital-acquired infections seems to be highly justified [13,14,15,16]. The biocidal properties of such materials were described in numerous publications; however, to our surprise, the antibacterial properties of pristine AAO seem to be not well discovered. Typically, the antibacterial properties of aluminium(III) oxide are reported for its nanoparticle form [17]. In contrast, some publications describe AAO matrices as neutral to microorganisms [10,18]. This statement, however, contradicts the results obtained in our experimental work: The pristine AAO matrices are definitely not inert to bacteria. For this reason, studying the antimicrobial properties of pure anodised alumina oxide seems to be a vital task.
Moreover, anodised aluminum oxide matrices can also be used in systems where the anticoagulant, hemodynamic and redox properties of water are analysed—soluble derivatives of Piloty’s acid which can efficiently release azanone (HNO) at pH 7.4 and 37 ∘ C (HNO donors). Thrombotic events are one of the leading causes of morbidity and mortality worldwide [19,20]. Therefore, intensive research is needed to develop new agents for the prevention of thrombotic events. Based on model bacterial systems, anodised aluminum oxide may be helpful in understanding the mechanisms regulating thrombotic events and in searching for new drugs capable of modulating molecular interactions between the endothelium and blood cells. They can also participate in gas exchange as new potential gas transmitters in the modulation of physiological processes related to hemostasis, hemodynamics and even metastasis [21].
In this consistent study, we undertake this topic and analyse the antimicrobial action of anodised aluminium(III) oxide membranes in detail. To be more thorough, we studied the response of four strains of Escherichia coli bacteria. Moreover, the tests were conducted for three antibiotics, ciprofloxacin (cipro), bleomycin (bleo) and cloxacillin (clox) and compared to the AAO matrices.
The experimental material undergone within the framework of this study was AAO matrices containing approximately 34–47 nm pores, arranged in regular 2D hexagonal order as shown in Figure 1. In order to exclude the influence of any harmful reagents on the antimicrobial properties of AAO, we also investigated two reference materials. They were similar AAO matrices with omitted one or two steps of the synthesis in order to avoid using chlorine or chromium which could potentially influence the results even in a trace amount. Such simplified syntheses resulted in a slightly different structure of materials. For details, see Section 2.

2. Materials and Methods

The anodised aluminium(III) oxide membranes were prepared in three ways:
  • A standard two-step anodisation with the use of perchloric acid (HClO 4 , 60% pure p. a., Chempur, Poland) and chromium (VI) oxide (CrO 3 , pure p. a., Chempur, Poland) compounds for electropolishing and the removal of the first-step Al 2 O 3 , respectively. This sample is denoted as AAO Cl+Cr, and its structure can be seen in Figure 1.
  • A matrix prepared in a one-step anodisation procedure with the omission of the preliminary anodisation to avoid the necessity of the removal of aluminium oxide layer with the use of the chromium compound. This sample is denoted as AAO Cl,
  • A matrix prepared without electropolishing in a one-step anodisation procdure. The fabrication of this type of sample requires none of the chlorine and chromium compounds. This sample is denoted as AAO Clean.
The procedure for the fabrication of AAO Cl+Cr can be found in our previous work on the antibacterial properties of AAO membranes with chemically attached functional groups [11]. In short, aluminium foil (99%, SmartMembranes, Halle, Germany) was cut into 3 cm × 3 cm squares and heated at 550 ∘ C for 12 h in a nitrogen atmosphere. Next, the foils were electropolished in a perchloric acid solution (HClO 4 , 60 % pure p. a., Chempur, Piekary Śląskie, Poland) at 20 V for 15 min.
The preliminary anodisation was conducted in 0.3 M oxalic acid (C 2 H 2 O 4 × 2H 2 O, a standard solution, prepared from 99.5 % pure reagent, (Chempur, Poland) at 20 V for two hours. This process is done to create a regular template on the surface. Next, the anodised aluminium oxide layer was removed in a CrO 3 solution at 60 ∘ C for two hours.
The main anodisation procedure was performed according to the same procedure as the preliminary anodisation with the exception that its duration was extended to 24 h.
The AAO Cl sample was prepared with the omission of the electropolishing step to avoid using the perchloric acid solution.
Finally, the AAO Clean sample was prepared without electropolishing and preliminary anodisation steps. The washed aluminium foil was used after the thermal treatment only for the main anodisation at the same conditions as previous samples.
The membranes were analysed regarding their morphology and chemical composition using scanning electron microcopy (SEM) TESCAN VEGA3 (TESCAN, Czech Republic) and energy-dispersive X-ray spectroscopy (EDS). The micrographs were taken at 7 kV and 12 beam intensity at the distance from the sample of 4 mm. EDS measurements were performed at 10 kV, the magnification of 200× and the distance from the sample of 12 mm. The diameters and pore distance were calculated by manually measuring at least 100 of each from SEM micrographs using ImageJ v. 1.53t [22].

2.1. Microorganisms and Media

Escherichia coli strains R1–R4 and K-12 were obtained as a kind gift from Professor Jolanta Łukasiewicz at the Ludwik Hirszfeld Institute of Immunology and Experimental Therapy (Polish Academy of Sciences). The reference bacterial strains of E. coli (K12 ATCC 25404, R2 ATCC 39544, R3 ATCC 11775, R4 ATCC 39543) were provided by LGC Standards (U.K.) and were used according to the recommendation of ISO 11133: 2014. These strains were used to test the antibacterial activity of the synthesised agents [20,21,22,23,24,25,26,27,28,29,30]. Bacteria were cultivated in a tryptic soy broth (TSB; Sigma-Aldrich, Saint Louis, MI, USA) liquid medium and on agar plates containing TSB medium at 25 ∘ C. Alternatively, TSB agar plates were used. The specific growth rate (μ), according to first-order kinetics, was measured using a microplate reader (Thermo, Multiskan FC, Vantaa, Finland) at 605 nm in the TSB medium. Lanes 1kb-ladder and Quick Extend DNA ladder (New England Biolabs, Ipswich, MA, USA) were used for MIC and MBC tests as described in detail in our previous works [20,21,22,23,24,25,26,27,28,29,30] and analysed by the Tukey test indicated by * p < 0.05, ** p < 0.1, *** p < 0.01. Model strains of E. coli were plotted in all 48-well plates observed; K12, R2-R4 which were treated with the analysed compounds. From the analysis of the MIC and MBC assays, colour changes were observed for all the compounds tested but at different levels and at different dilutions. Bacterial strains R3 and R4 were the most susceptible to modification with these compounds due to the increasing length of their LPS (visible dilutions of 10 − 3 corresponding to the concentration of 0.25 μM) compared to K12 and R2 strains (visible dilutions of 10 − 6 corresponding to a concentration of 0.06 μM). The analysed R4 strain was the most sensitive, most likely due to the longest length of the lipopolysaccharide chain in the bacterial membrane. In all cases, the MBC values were approximately 170 times higher than the MIC values.

2.2. Estimation of Minimum Inhibition Concentration and Minimum Bactericidal Concentration

A drug or compound (visible dilutions of 10 − 2 corresponding to the concentration of 0.0015 μM) directly kills the vegetative forms of the bacteria. The obtained results depict that at all studied concentrations, AAO have an inhibitory effect on each studied bacterial model. Varied inhibitory activity was noted depending on the nature of the substituent in the aromatic ring of the tested compounds.
MICs and MBCs were determined using the methodology described previously [20,21,22,23,24,25,26,27,28,29,30] with serial dilutions of 10 − 1 to 10 − 7 . In short, the analysed model E. coli strains were grown in a TSB medium and diluted in water. Next, 50 μL TSB medium was inoculated with 10 6 cfu/mL (CFU, colony-forming units, approximately 0.5 McFarland units) of the bacterial strains. Next, the bacteria were transferred onto AAO matrices and incubated with each at a concentration of 1 mM for 24 h at 37 ∘ C. Next, 50 μL of the bacterial suspension from all the analysed membranes was diluted to 0.1 mM and plated on a 48-well plate (according to the markings) with resazurin (0.02 mg/mL), which was added to all the wells, as an indicator. The plates were incubated at 30 ∘ C for 24 h. Colour changes from blue to pink or yellowish with turbidity were considered positive. The lowest concentration at which there was no visible colour change was set as the MIC.
The MBC was estimated based on the measurement of the bacterial activity after a 24 h incubation with or without the AAO matrices. The lowest concentration at which there was no visible red colour was taken as the MBC.
An example of the analysis of test compounds of various concentrations using a resazurin dye on microplates (mg/L) is presented in Figure S1 (Supplementary Materials). Experiments were performed with three independent replicates.
The bacterial DNA was isolated from cultures of K12, R2, R3 and R4 E. coli as described in [20,21,22,23,24,25,26,27,28,29,30].
All microorganisms and media were precisely described in the previous works [20,21,22,23,24,25,26,27,28,29,30] and analysed by the Tukey test indicated by (p < 0.05): * p < 0.05, ** p < 0.1, *** p < 0.01, Table 1). ANOVA tests were used for the statistical analysis of the biological assay results using the JMP software [31]. The error bars in the graphs represent the standard deviation of the appropriate result.

3. Results and Discussion

3.1. Microscopic Observations

The anodised membranes were characterised just after the syntheses using SEM regarding their morphology (Figure 2). The anodisation without electrochemical preparation of a substrate resulted in the formation of irregular pores covering only parts of the surface. Stripes present on the surface deriving from the foil processing influence the location of the pores due to different surface energy levels (Figure 2a). The average size of the pores and the distance between them are the smallest from other membranes with the largest deviation (relative to the size, Table 2). Electropolishing aluminium foil prior to anodisation results in significantly more pores covering the whole surface (Figure 2b). The one-step anodisation produced relatively irregular pores (in comparison to the two-step anodisation): Twin or triplet pore formation in one cell occurs more often. The increased average distance between pores compared to AAO Clean indicates the formation of proper anodisation cells for each pore or pore system (twins, triplets). Finally, the membranes fabricated with the use of the two-step anodisation method consist of fairly ordered pores within each aluminium grain. The size of the pores is almost the same as for the electropolished membrane. The average distance between the pores is the same as for the one-step anodisation membrane. However, the standard deviation is approximately 54% smaller indicating significantly more orderly placed pores on the surface.
The EDS measurements revealed no chlorine and no chromium contamination in any of the samples. The minimal values for the two elements visible in Table 3 are the result of forcing the software to search for their corresponding peaks. The identified gold derives from the evaporated conductive layer for SEM studies. The values are presented as detected without rounding for visibility.

3.2. Antibacterial Properties

The model bacterial strains of E. coli K12, R2-R4, were treated with the analysed anodised aluminum oxide using MIC and MBC assays with 48-well plates. Colour changes were observed for all tested compounds but at different levels and at different dilutions. The most susceptible to modification with these compounds were the bacterial strains R3 and R4 due to the increased length of their lipopolysaccharides (LPS, visible dilutions 10 − 2 corresponding to the concentration of 0.0015 μM) in comparison to strains K12 and R2 (visible dilutions 10 − 6 corresponding to the concentration of 0.0015 μM and 0.02 μM for K12 and R2, respectively, Figure 3 and Table 1). The analysed R4 strain was the most sensitive of all strains most likely due to the longest length of the lipopolysaccharide chain in the bacterial membrane which may curl relaxing depending on the used material.
The analysed K12 and R2–R4 strains of Escherichia coli are not only the dominant species of the human aerobic bacterial microbiota and microbiota of various habitats in which people live, e.g., bathrooms, clinics and hospitals, but they can also temporarily colonise the oropharynx and skin of healthy people. However, apart from saprophytic strains (harmless to humans), there are also strains of E. coli which are pathogenic for humans and cause various forms of acute diarrhoea. Infection usually occurs through contaminated food and water, as in the case with other bacterial diarrhoea, and (less frequently) through indirect contact. In industrialised countries, pathogenic enteric strains of Escherichia coli are rarely part of the intestinal microbiota of healthy people; therefore, they are considered strictly pathogenic. When the appropriate amount of bacteria is ingested by a person susceptible to pathogenic infection, strains of E. coli have the ability to cause inflammation of the small and/or large intestine. Adequate gastric acidity has a disinfecting effect and protects (to a certain extent) against infection. Therefore, people with low gastric acidity are particularly susceptible to infections with pathogenic Escherichia coli strains. The source of the infection is a sick person or a vector (except for STEC/EHEC strains, for which cattle are the source). Intestinal diseases caused by pathogenic strains of E. coli occur in the form of epidemics or, in rare cases, with an increase in the incidence in the summer months which is the rule for bacterial diarrhoea. Therefore, we studied these strains with the use of the AAO matrices to investigate their etiology and the mechanism causing their resistance to many known and commonly used antibiotics. Despite the number of reports in the literature on the pharmacological and biological properties of AAO matrices, their antibacterial activities are still being rediscovered. Thus, there exists a need for additional research on their cytotoxic effect on selected hospital bacterial strains causing diseases associated with blood infections such as sepsis. The AAO matrices still need to be further studied in vivo to better delineate the pharmacological potential of this class of substances, so there is a need to clarify their role [23,24,25,26,27,28,29,30,32,33,34].
In all the analysed cases, the MBC values were approximately 260 times higher than the MIC values (Figure 3). Based on the observed results in the MIC and MBC tests, it was found that the analysed AAO matrices significantly influenced the fragmentation of the membrane and the structure of the cell wall of bacteria containing LPS of various lengths [23,24,25,26]. This causes high oxidative stress in the cell which affects the damage and modification of bacterial DNA caused by the analysed anodised aluminum oxide compounds. This was additionally confirmed by the digestion with the specific enzyme Fpg of modified bacterial DNA (Labjot, New England Biolabs, UK) which is a marker of oxidative stress [27,28,29,30,32,33,34,35] recognising oxidised guanine and adenine (Figure 4).
The highest values after the application of the membranes in model strains of E. coli after the use of MIC, MBC, and MBC/MIC tests were observed after treatment with a mixture of chlorine with chromium and with chlorine alone. They were increased in all strains and comparable with each other after the use of chlorine alone or chlorine with chromium and their highest value was observed for the R4 strain whose LPS length was the longest and was relaxed inducing significant oxidative stress in the cell. A similar effect was observed after the strains were modified with the AAO membranes and treated with the Fpg protein.
In reference to our previous work related to the analysis of functionalised anodised aluminum oxide [11], we observed a clear effect of their action on selected bacterial strains. On the other hand, compared to the analysed nonfunctionalised anodised aluminum oxide, we found similar but lower values for the model bacterial slippers. This may be due to the fact that the LPS, contained in the bacterial membrane, under the influence of the functionalised membranes can fold in a specific way creating conformations that are pulled out of the cell on the “flip out” principle. This drastically and rapidly changes the electrokinetic potential of the membrane increasing the level of stress oxidative activity in the cell which disrupts its homeostasis.
Located in the bacterial membrane, the outward LPS covers part of it by the “passivation” principle, which can greatly facilitate the accessibility of a given compound to interact with and act on it (Figure 5) because the R4 strain has the longest LPS and the most labile, as described in detail in the work of Prosts [26] and Maciejewska [25]. A similar effect was observed in the works of Kowalczyk [23] and Borkowski [24] after treatment with quaternary ammonium ionic liquids on selected model strains of E. coli where a clear change in the electrokinetic potential of the membrane was observed. The interaction of the applied functionalised membranes on the surface of the E. coli cell already generates strong oxidative stress in it, where there is a strong fragmentation of bacterial DNA after treatment with the Fpg protein from the group of repair glycosylases, as it was described later in the results and their discussion.
The obtained values of oxidative damage after digestion with Fpg protein in bacterial DNA were compared with modifications of bacterial DNA after treatment with antibiotics: ciprofloxacin, bleomycin and cloxacillin [23,24,25,26]. The presented research shows that the analysed compounds will be used in the future as typical “substitutes” for new drugs in relation to antibiotics used in hospital infections. The obtained MIC values, as well as our previous studies with various types of the analysed compounds [23,24,25,26], indicate that they show a strong cytotoxic effect on the analysed model bacterial strains K12 and R2–R4 (Figure 6).
Based on the MIC and MBC values, the analysed compounds were selected for further studies (on the basis of their highest biological activity similar to antibiotics) for the analysis of oxidative stress in the cell by modifying their bacterial DNA. Using the Fpg protein, we wanted to observe whether the resulting modifications in bacterial DNA would introduce oxidative damage to the DNA strand by altering the topological three forms of bacterial DNA; ccc, oc and linear forms. As observed in previous studies [27,28,29,30,32,33,34,35], the results of bacterial DNA modified with the analysed compounds (Figure 5) showed that all analysed compounds with different types of localised substituents can strongly change the bacterial DNA topology, even after digestion with Fpg protein based on agarose gels.
Changes in the main topological forms of the plasmid: ccc, oc and linear were observed in DNA isolated from model strains and digested with Fpg protein. Approximately 3.5% of oxidative damage was identified after digestion with the Fpg protein which indicates that the analysed compounds damage bacterial DNA very strongly similar to the observations of previous studies [27,28,29,30,32,33,34,35] in the tested bacterial strains, in particular R4, as evidenced by the obtained MIC and MBC values which were statistically significant at p < 0.05 (Figure 6 and Figure 7).

4. Conclusions

Anodic aluminium oxide membranes exhibit strong antibacterial properties comparable to some of the tested antibiotics. Despite no detected chlorine and chromium content, the standard two-step anodisation membrane, AAO Cl+Cr, is the most efficient material. The toxicity of the analysed membranes with the appropriate strains of bacteria depends on their interaction with the membrane, which may affect the destruction of cell walls by changing their hydrophobicity. Changes in the permeability and integrity of the bacterial membrane may result in a specific bacterial response to biologically active compounds, e.g., antibiotics used. This may suggest that the presence of these compounds has a toxic effect on bacterial LPS, generating strong oxidative stress, which we observed in our previous studies [20,21,22,23,24,25,26,27,28,29,30]. The results of the presented studies are important for understanding the biological properties of the tested membranes as a function of potential new antibiotics such as kanamycin, streptomycin, ciprofloxacin, bleomycin and cloxacillin, commonly used in nosocomial infections with pathogenic strains causing various diseases: syphilis, sepsis or psoriasis. The use of membranes will multiply their toxic effect on Gram-negative and Gram-positive bacteria in the face of the growing drug resistance pandemic.
Based on our knowledge, we can precisely design special membranes with high selectivity of tissue functions. Currently, one of the most important research tasks is a broader understanding of the mechanisms of interaction of these compounds on various structures, both at the cellular and molecular level. Particular attention should be paid to their use in air conditioners located in offices, hospitals or food rooms.
There is still a need for a more fundamental understanding of the performance of already synthesised and newly designed membranes in vivo using high-throughput bacterial screening in both Gram-positive and negative systems. Another practical application of the different membranes will be understanding their physiological effects under environmental conditions. In order to introduce them commercially to the market, further research is required on their biostability, half-life, preferred method of administration and potency on the analysed bacterial, fungal or viral strains.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/molecules28010401/s1, Figure S1: Examples of MIC and MBC on microplates with different concentrations of studied compounds (μg/mL). The numbers correspond to sample names 1 = AAO Clean, 2 = AAO Cl, 3 = AAO Cl+Cr. Resazurin was added as an indicator of microbial growth with K12, R2, R3, and R4 strains with tested AAO matrices (for which the statistical analysis is shown in Table 1). Additionally, examples of MIC with different strains K12, R2, R3, and R4 of studied antibiotics with ciprofloxacin (ci), bleomycin (b), and cloxacillin (cl) in (μ/mL). Figure S2: An example of an agarose gel electrophoresis separation of isolated plasmids DNA from selected R4 E. coli strains reacted with different AAO membranes (upper picture). 0 = normal plasmid not treated with AAO membranes, 1 = plasmid treated with clean AAO Clean, 2 = plasmid treated with AAO Cl, 3 = plasmid treated with AAO Cl+Cr, as shown in Figure 4, and digested with repair Fpg protein (lower picture). M = marker. 1 = plasmid treated with clean AAO Clean, 2 = plasmid treated with AAO Cl, 3 = plasmid treated with AAO Cl+Cr. Figure S3: Example of an agarose gel electrophoresis separation of isolated plasmids DNA from K12 and R4 strains modified with antibiotics: bleomycin, ciprofloxacin, and cloxacillin digested (or not) with repair enzymes Fpg. M = marker.

Author Contributions

Conceptualization, M.S., P.K. and Ł.L.; Methodology, M.S., P.K., Ł.L., B.G., A.W., K.K. and K.W.; Investigation, M.S., P.K., A.K., B.G., A.W., K.K., K.W. and Ł.L.; Resources, M.S., P.K., A.K., B.G., A.W., K.K., K.W. and Ł.L.; Data curation, M.S., P.K. and Ł.L.; Writing—original draft preparation, M.S., Ł.L., P.K. and A.K.; Writing—review and editing, M.S., P.K. and Ł.L.; Supervision, P.K., Ł.L. and M.S.; Funding acquisition, Ł.L., M.S. and K.K.; Project administration, Ł.L.; Validation, A.W., B.G. and K.W.; Visualisation, M.S., P.K. and Ł.L. All authors have read and agreed to the published version of the manuscript.

Funding

This work was supported by the National Science Centre, Poland, grants 2017/26/E/ST5/00162, 2020/04/X/ST5/00127, and by the grant of the Medical University of Białystok, Poland, SUB/2/DN/22/001/2201.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Acknowledgments

We are grateful to Anna Kula from AGH University of Science and Technology for the EDS analysis. Moreover, we would like to thank Jolanta Łukasiewicz from Ludwik Hirszfeld Institute of Immunology and Experimental Therapy Polish Academy of Sciences for providing the strains of E. coli.

Conflicts of Interest

The authors declare no conflict of interest.

Abbreviations

The following abbreviations are used in this manuscript:
AAOAnodised aluminium oxide
LPSLipopolysacchrides
SEMScanning electron microscopy
EDSEnergy-dispersive X-ray spectroscopy
CFUColony-forming units

References

  1. Masuda, H.; Yada, K.; Osaka, A. Self-ordering of cell configuration of anodic porous alumina with large-size pores in phosphoric acid solution. Jpn. J. Appl. Phys. 1998, 37, L1340. [Google Scholar] [CrossRef]
  2. Liu, S.; Tian, J.; Zhang, W. Fabrication and application of nanoporous anodic aluminum oxide: A review. Nanotechnology 2021, 32, 222001. [Google Scholar] [CrossRef] [PubMed]
  3. Poinern, G.E.J.; Ali, N.; Fawcett, D. Progress in nano-engineered anodic aluminum oxide membrane development. Materials 2011, 4, 487–526. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  4. Ingham, C.J.; ter Maat, J.; de Vos, W.M. Where bio meets nano: The many uses for nanoporous aluminum oxide in biotechnology. Biotechnol. Adv. 2012, 30, 1089–1099. [Google Scholar] [CrossRef] [PubMed]
  5. Toth, R.; Schabikowski, M.; Heier, J.; Braun, A.; Kata, D.; Graule, T. The effect of solvent and electric field on the size distribution of iron oxide microdots: Exploitation of self-assembly strategies for photoelectrodes. J. Mater. Res. 2011, 26, 254–261. [Google Scholar] [CrossRef] [Green Version]
  6. Laskowski, Ł.; Laskowska, M.; Dulski, M.; Zubko, M.; Jelonkiewicz, J.; Perzanowski, M.; Vilà, N.; Walcarius, A. Multi-step functionalization procedure for fabrication of vertically aligned mesoporous silica thin films with metal-containing molecules localized at the pores bottom. Microporous Mesoporous Mater. 2019, 274, 356–362. [Google Scholar]
  7. Laskowska, M.; Dulski, M.; Marszałek, M.; Zubko, M.; Laskowski, Ł. Vertically aligned porous silica thin films functionalized by nickel chloride incorporated in walls. Microporous Mesoporous Mater. 2019, 276, 201–206. [Google Scholar] [CrossRef]
  8. Schabikowski, M.; Zalewska, M.; Kata, D.; Graule, T. The effect of CuO coatings on the electrokinetic properties of stone wool fibres determined by streaming potential measurements. Ceram. Int. 2016, 42, 13944–13951. [Google Scholar] [CrossRef]
  9. Liu, L.; Lee, W.; Huang, Z.; Scholz, R.; Gösele, U. Fabrication and characterization of a flow-through nanoporous gold nanowire/AAO composite membrane. Nanotechnology 2008, 19, 335604. [Google Scholar] [CrossRef]
  10. Zhang, Y.M.; Hu, Y.; Wang, G.Q.; Gui, Y.; Wang, D.F.; Huang, J.Q.; Jie, X.H. Preparation and antimicrobial properties of surface antibacterial layer of aluminum. In Proceedings of the Advanced Materials Research; Trans Tech Publishers: Zurich, Switzerland, 2014; Volume 941, pp. 1659–1663. [Google Scholar]
  11. Schabikowski, M.; Laskowska, M.; Kowalczyk, P.; Fedorchuk, A.; Szőri-Dorogházi, E.; Németh, Z.; Kuźma, D.; Gawdzik, B.; Wypych, A.; Kramkowski, K.; et al. Functionalised Anodised Aluminium Oxide as a Biocidal Agent. Int. J. Mol. Sci. 2022, 23, 8327. [Google Scholar] [CrossRef]
  12. Laskowski, L.; Laskowska, M.; Fijalkowski, K.; Piech, H.; Jelonkiewicz, J.; Jaskulak, M.; Gnatowski, A.; Dulski, M. New class of antimicrobial agents: SBA-15 silica containing anchored copper ions. J. Nanomater. 2017, 2017. [Google Scholar] [CrossRef]
  13. Querido, M.M.; Aguiar, L.; Neves, P.; Pereira, C.C.; Teixeira, J.P. Self-disinfecting surfaces and infection control. Colloids Surf. Biointerfaces 2019, 178, 8–21. [Google Scholar] [CrossRef]
  14. Sehmi, S.K.; Lourenco, C.; Alkhuder, K.; Pike, S.D.; Noimark, S.; Williams, C.K.; Shaffer, M.S.; Parkin, I.P.; MacRobert, A.J.; Allan, E. Antibacterial surfaces with activity against antimicrobial resistant bacterial pathogens and endospores. ACS Infect. Dis. 2020, 6, 939–946. [Google Scholar] [CrossRef]
  15. Mikelonis, A.M.; Rowles, L.S.; Lawler, D.F. The effects of water chemistry on the detachment and dissolution of differently stabilized silver nanoparticles from ceramic membranes. Environ. Sci. Water Res. Technol. 2020, 6, 1347–1356. [Google Scholar] [CrossRef]
  16. Brüggemann, D. Nanoporous aluminium oxide membranes as cell interfaces. J. Nanomater. 2013, 2013, 32. [Google Scholar] [CrossRef]
  17. Ansari, M.A.; Khan, H.M.; Alzohairy, M.A.; Jalal, M.; Ali, S.G.; Pal, R.; Musarrat, J. Green synthesis of Al2O3 nanoparticles and their bactericidal potential against clinical isolates of multi-drug resistant Pseudomonas aeruginosa. World J. Microbiol. Biotechnol. 2015, 31, 153–164. [Google Scholar] [CrossRef] [PubMed]
  18. Pornnumpa, N.; Jariyaboon, M. Antibacterial and corrosion resistance properties of anodized AA6061 aluminum alloy. Eng. J. 2019, 23, 171–181. [Google Scholar] [CrossRef]
  19. Gade, I.L.; Brækkan, S.; Næss, I.A.; Hansen, J.B.; Rosendaal, F.; Cannegieter, S.; Overvad, K.; Jensvoll, H.; Hammerstrøm, J.; Gran, O.V.; et al. Epidemiology of venous thromboembolism in hematological cancers: The Scandinavian Thrombosis and Cancer (STAC) cohort. Thromb. Res. 2017, 158, 157–160. [Google Scholar] [CrossRef]
  20. Raskob, G.E.; Angchaisuksiri, P.; Blanco, A.N.; Buller, H.; Gallus, A.; Hunt, B.J.; Hylek, E.M.; Kakkar, A.; Konstantinides, S.V.; McCumber, M.; et al. Thrombosis: A major contributor to global disease burden. Arterioscler. Thromb. Vasc. Biol. 2014, 34, 2363–2371. [Google Scholar] [CrossRef] [Green Version]
  21. Andreadou, I.; Iliodromitis, E.K.; Rassaf, T.; Schulz, R.; Papapetropoulos, A.; Ferdinandy, P. The role of gasotransmitters NO, H2S and CO in myocardial ischaemia/reperfusion injury and cardioprotection by preconditioning, postconditioning and remote conditioning. Br. J. Pharmacol. 2015, 172, 1587–1606. [Google Scholar] [CrossRef] [Green Version]
  22. Schneider, C.A.; Rasband, W.S.; Eliceiri, K.W. NIH Image to ImageJ: 25 years of image analysis. Nat. Methods 2012, 9, 671–675. [Google Scholar] [CrossRef] [PubMed]
  23. Kowalczyk, P.; Borkowski, A.; Czerwonka, G.; Cłapa, T.; Cieśla, J.; Misiewicz, A.; Borowiec, M.; Szala, M. The microbial toxicity of quaternary ammonium ionic liquids is dependent on the type of lipopolysaccharide. J. Mol. Liq. 2018, 266, 540–547. [Google Scholar] [CrossRef]
  24. Borkowski, A.; Kowalczyk, P.; Czerwonka, G.; Cieśla, J.; Cłapa, T.; Misiewicz, A.; Szala, M.; Drabik, M. Interaction of quaternary ammonium ionic liquids with bacterial membranes–Studies with Escherichia coli R1–R4-type lipopolysaccharides. J. Mol. Liq. 2017, 246, 282–289. [Google Scholar] [CrossRef]
  25. Maciejewska, A.; Kaszowska, M.; Jachymek, W.; Lugowski, C.; Lukasiewicz, J. Lipopolysaccharide-linked Enterobacterial Common Antigen (ECALPS) Occurs in Rough Strains of Escherichia coli R1, R2, and R4. Int. J. Mol. Sci. 2020, 21, 6038. [Google Scholar] [CrossRef] [PubMed]
  26. Prost, M.; Prost, R. Basic parameters of evaluation of the effectiveness of antibiotic therapy. OphthaTherapy 2017, 4, 233–236. [Google Scholar] [CrossRef]
  27. Kowalczyk, P.; Wilk, M.; Parul, P.; Szymczak, M.; Kramkowski, K.; Raj, S.; Skiba, G.; Sulejczak, D.; Kleczkowska, P.; Ostaszewski, R. The Synthesis and Evaluation of Aminocoumarin Peptidomimetics as Cytotoxic Agents on Model Bacterial E. coli Strains. Materials 2021, 14, 5725. [Google Scholar] [CrossRef]
  28. Samsonowicz-Górski, J.; Kowalczyk, P.; Koszelewski, D.; Brodzka, A.; Szymczak, M.; Kramkowski, K.; Ostaszewski, R. The Synthesis and Evaluation of Amidoximes as Cytotoxic Agents on Model Bacterial E. coli Strains. Materials 2021, 14, 7577. [Google Scholar] [CrossRef]
  29. Kowalczyk, P.; Trzepizur, D.; Szymczak, M.; Skiba, G.; Kramkowski, K.; Ostaszewski, R. 1, 2-Diarylethanols—A New Class of Compounds That Are Toxic to E. coli K12, R2–R4 Strains. Materials 2021, 14, 1025. [Google Scholar] [CrossRef]
  30. Kowalczyk, P.; Madej, A.; Szymczak, M.; Ostaszewski, R. α-Amidoamids as New Replacements of Antibiotics—Research on the Chosen K12, R2–R4 E. coli Strains. Materials 2020, 13, 5169. [Google Scholar] [CrossRef]
  31. Available online: https://www.jmp.com/en_gb/home.html (accessed on 1 October 2022).
  32. Kowalczyk, P.; Gawdzik, B.; Trzepizur, D.; Szymczak, M.; Skiba, G.; Raj, S.; Kramkowski, K.; Lizut, R.; Ostaszewski, R. δ-Lactones—A New Class of Compounds That Are Toxic to E. coli K12 and R2–R4 Strains. Materials 2021, 14, 2956. [Google Scholar] [CrossRef]
  33. Gawdzik, B.; Kowalczyk, P.; Koszelewski, D.; Brodzka, A.; Masternak, J.; Kramkowski, K.; Wypych, A.; Ostaszewski, R. The Evaluation of DHPMs as Biotoxic Agents on Pathogen Bacterial Membranes. Membranes 2022, 12, 238. [Google Scholar] [CrossRef] [PubMed]
  34. Sahrawat, P.; Kowalczyk, P.; Koszelewski, D.; Szymczak, M.; Kramkowski, K.; Wypych, A.; Ostaszewski, R. Influence of Open Chain and Cyclic Structure of Peptidomimetics on Antibacterial Activity in E. coli Strains. Molecules 2022, 27, 3633. [Google Scholar] [CrossRef] [PubMed]
  35. Kowalczyk, P.; Koszelewski, D.; Gawdzik, B.; Samsonowicz-Górski, J.; Kramkowski, K.; Wypych, A.; Lizut, R.; Ostaszewski, R. Promiscuous Lipase-Catalyzed Markovnikov Addition of H-Phosphites to Vinyl Esters for the Synthesis of Cytotoxic α-Acyloxy Phosphonate Derivatives. Materials 2022, 15, 1975. [Google Scholar] [CrossRef] [PubMed]
Molecules 28 00401 g001 550
Figure 1. The schematic representation of the structure of an anodised aluminium oxide membrane.
Figure 1. The schematic representation of the structure of an anodised aluminium oxide membrane.
Molecules 28 00401 g001
Molecules 28 00401 g002 550
Figure 2. Scanning electron micrographs of the anodised membranes: (a) AAO Clean, (b) AAO Cl and (c) AAO Cl+Cr.
Figure 2. Scanning electron micrographs of the anodised membranes: (a) AAO Clean, (b) AAO Cl and (c) AAO Cl+Cr.
Molecules 28 00401 g002
Molecules 28 00401 g003 550
Figure 3. The antibacterial properties of the AAO membranes: (a) minimal inhibitory concentration of the AAO membranes in model bacterial strains, (b) minimal bactericidal concentration of the amidoximes in model bacterial strains, (c) MBC/MIC of the coumarin derivatives. Each experiment was performed independently in three replications (n = 3).
Figure 3. The antibacterial properties of the AAO membranes: (a) minimal inhibitory concentration of the AAO membranes in model bacterial strains, (b) minimal bactericidal concentration of the amidoximes in model bacterial strains, (c) MBC/MIC of the coumarin derivatives. Each experiment was performed independently in three replications (n = 3).
Molecules 28 00401 g003
Molecules 28 00401 g004 550
Figure 4. Percentage of plasmid DNA recognised by Fpg enzyme (y-axis) with model bacteria, K12, and R2–R4 strains (x-axis). Each experiment was performed independently in three replications (n = 3).
Figure 4. Percentage of plasmid DNA recognised by Fpg enzyme (y-axis) with model bacteria, K12, and R2–R4 strains (x-axis). Each experiment was performed independently in three replications (n = 3).
Molecules 28 00401 g004
Molecules 28 00401 g005 550
Figure 5. The proposed mechanism of antibacterial action of functionalised and nonfunctionalised anodised aluminum oxide in selected model E. coli strains.
Figure 5. The proposed mechanism of antibacterial action of functionalised and nonfunctionalised anodised aluminum oxide in selected model E. coli strains.
Molecules 28 00401 g005
Molecules 28 00401 g006 550
Figure 6. Examples of MIC with model bacterial strains K12, R2, R3, and R4 with ciprofloxacin, bleomycin, and cloxacillin. Each experiment was performed independently in three replications (n = 3).
Figure 6. Examples of MIC with model bacterial strains K12, R2, R3, and R4 with ciprofloxacin, bleomycin, and cloxacillin. Each experiment was performed independently in three replications (n = 3).
Molecules 28 00401 g006
Molecules 28 00401 g007 550
Figure 7. The percentage of bacterial DNA recognised by Fpg enzyme in model bacterial strains after ciprofloxacin, bleomycin, and cloxacillin treatment. The compounds were statistically significant at p < 0.05. Each experiment was performed independently in three replications (n = 3).
Figure 7. The percentage of bacterial DNA recognised by Fpg enzyme in model bacterial strains after ciprofloxacin, bleomycin, and cloxacillin treatment. The compounds were statistically significant at p < 0.05. Each experiment was performed independently in three replications (n = 3).
Molecules 28 00401 g007
Table
Table 1. The statistical analysis of the measured membranes by MIC, MBC and MBC/MIC; <0.05 *, <0.01 **, <0.001 ***.
Table 1. The statistical analysis of the measured membranes by MIC, MBC and MBC/MIC; <0.05 *, <0.01 **, <0.001 ***.
StrainAAO CleanAAO + ClAAO Cl+CrType of Test
K12*****MIC
R2*****MIC
R3*****MIC
R4*****MIC
K12*******MBC
R2*******MBC
R3*******MBC
R4*******MBC
K12****MBC/MIC
R2****MBC/MIC
R3****MBC/MIC
R4****MBC/MIC
Table
Table 2. The pore size and average distance between them for the anodised membranes.
Table 2. The pore size and average distance between them for the anodised membranes.
SamplePore Size [nm]Distance between Pores [nm]
AAO Clean34 ± 980 ± 19
AAO Cl44 ± 12103 ± 13
AAO Cl+Cr47 ± 12103 ± 6
Table
Table 3. The chemical analysis of the AAO Cl+Cr membrane.
Table 3. The chemical analysis of the AAO Cl+Cr membrane.
ElementC-KO-KAl-KCl-KCr-KAu-M
Weight %2.88 ± 0.1627.12 ± 3.1734.12 ± 0.990.01 ± 0.010.02 ± 0.0235.85 ± 2.37
Atom %7.10 ± 0.3549.98 ± 3.3037.50 ± 2.620.01± 0.010.01 ± 0.035.41 ± 0.57
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.

Share and Cite

MDPI and ACS Style

Schabikowski, M.; Kowalczyk, P.; Karczmarska, A.; Gawdzik, B.; Wypych, A.; Kramkowski, K.; Wrzosek, K.; Laskowski, Ł. Aluminium(III) Oxide—The Silent Killer of Bacteria. Molecules 2023, 28, 401. https://doi.org/10.3390/molecules28010401

AMA Style

Schabikowski M, Kowalczyk P, Karczmarska A, Gawdzik B, Wypych A, Kramkowski K, Wrzosek K, Laskowski Ł. Aluminium(III) Oxide—The Silent Killer of Bacteria. Molecules. 2023; 28(1):401. https://doi.org/10.3390/molecules28010401

Chicago/Turabian Style

Schabikowski, Mateusz, Paweł Kowalczyk, Agnieszka Karczmarska, Barbara Gawdzik, Aleksandra Wypych, Karol Kramkowski, Karol Wrzosek, and Łukasz Laskowski. 2023. "Aluminium(III) Oxide—The Silent Killer of Bacteria" Molecules 28, no. 1: 401. https://doi.org/10.3390/molecules28010401

APA Style

Schabikowski, M., Kowalczyk, P., Karczmarska, A., Gawdzik, B., Wypych, A., Kramkowski, K., Wrzosek, K., & Laskowski, Ł. (2023). Aluminium(III) Oxide—The Silent Killer of Bacteria. Molecules, 28(1), 401. https://doi.org/10.3390/molecules28010401

Article Metrics

Back to TopTop