Identification and Characterization of a Novel Endo-β-1,4-Xylanase from Streptomyces sp. T7 and Its Application in Xylo-Oligosaccharide Production
Abstract
:1. Introduction
2. Results and Discussion
2.1. Obtaining Xylanase Secreting Strains
2.2. Sequence and Structure Analysis
2.3. Expression and Purification
2.4. Biochemical Characteristics
2.5. Substrate Specificity and Kinetic Properties
2.6. XOS Production
3. Materials and Methods
3.1. Strains, Plasmids, and Chemicals
3.2. Microorganism Isolation and Identification
3.3. Sequence and Structure Analysis
3.4. Cloning and Recombinant Expression
3.5. Purification
3.6. Enzyme Activity Assay
3.7. Biochemical and Kinetic Characteristics
3.8. Production and Analysis of XOS
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Conflicts of Interest
References
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Metal Ions/Chemical Reagents | Relative Activity (%) | ||
---|---|---|---|
5 mM | 2.5 mM | 1 mM | |
KCl | 80.5 ± 0.59 | 87.2 ± 0.60 | 102.9 ± 0.62 |
NaCl | 92.9 ± 0.73 | 98.9 ± 0.40 | 99.7 ± 0.40 |
Ni2+ | 95.6 ± 0.49 | 97.7 ± 0.38 | 98.0 ± 0.17 |
CaCl2 | 86.7 ± 0.60 | 92.0 ± 0.39 | 95.1 ± 0.20 |
MnSO4 | 110.4 ± 1.70 | 109.9 ± 0.31 | 107.4 ± 0.29 |
FeSO4 | 43.9 ± 0.6 | 49.9 ± 0.68 | 85.9 ± 0.38 |
CoCl2 | 96.5 ± 0.73 | 99.5 ± 0.42 | 104.1 ± 0.38 |
MgCl2 | 86.2 ± 2.27 | 88.0 ± 1.86 | 101.2 ± 0.24 |
FeCl3 | 23.7 ± 2.97 | 25.8 ± 1.51 | 27.2 ± 0.28 |
EDTA | 24.8 ± 0.92 | 64.6 ± 1.04 | 82.5 ± 0.46 |
SDS | 23.2 ± 2.64 | 35.3 ± 1.32 | 41.7 ± 0.25 |
Substrate | Enzyme Assay | Kinetic Parameter | |||
---|---|---|---|---|---|
Enzyme | Activity Units (U/mL) | Km (mg/mL) | Vmax (U/mg mL) | kcat/Km (s−1·mg−1) | |
pNPA | α-arabinofuranosidase | <0.05 | NA | NA | NA |
pNPX | β-xylosidase | <0.05 | NA | NA | NA |
pNPGα | α-glucosidase | <0.05 | NA | NA | NA |
pNPGβ | β-glucosidase | <0.05 | NA | NA | NA |
CMC | Cellulase | <0.5 | NA | NA | NA |
Avicel | Cellulase | <0.5 | NA | NA | NA |
Corncob xylan | Xylanase | 235.8 ± 3.1 | 2.33 ± 0.15 | 678.41 ± 1.35 | 58.23 |
Birchwood xylan | Xylanase | 203.3 ± 2.8 | 2.78 ± 0.21 | 596.54 ± 2.12 | 42.91 |
Oat spelt xylan | Xylanase | 193.3 ± 2.5 | 3.12 ± 0.11 | 545.33 ±1.18 | 34.96 |
Beechwood xylan | Xylanase | 188.6 ± 3.6 | 3.54 ± 0.13 | 495.23 ± 1.87 | 27.98 |
Sources | pH | Thermostability | XOS Production | References | |
---|---|---|---|---|---|
Stability | (°C) | Main Type | Xylose | ||
Penicillium funiculosum | 4.0–5.5 | ≤70 | X2 | – | [38] |
Jonesia denitrificans | 6.0–9.0 | ≤55 | X4, X5, X6 | * | [39] |
Thermobacillus composti | 5.0–9.0 | ≤65 | X2, X3 | * | [40] |
Paludibacter propionicigenes | 4.0–9.0 | <50 | X2, X3 | * | [41] |
Streptomyces sp. B6 | 5.0–9.0 | ≤70 | X2, X3, X4 | ** | [42] |
Aspergillus fumigatus var. niveus | 4.5–7.0 | ≤70 | X2, X3 | – | [43] |
Streptomyces sp. T7 | 5.0–11.0 | ≤80 | X2, X3 | – | this paper |
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Li, Y.; Zhang, X.; Lu, C.; Lu, P.; Yin, C.; Ye, Z.; Huang, Z. Identification and Characterization of a Novel Endo-β-1,4-Xylanase from Streptomyces sp. T7 and Its Application in Xylo-Oligosaccharide Production. Molecules 2022, 27, 2516. https://doi.org/10.3390/molecules27082516
Li Y, Zhang X, Lu C, Lu P, Yin C, Ye Z, Huang Z. Identification and Characterization of a Novel Endo-β-1,4-Xylanase from Streptomyces sp. T7 and Its Application in Xylo-Oligosaccharide Production. Molecules. 2022; 27(8):2516. https://doi.org/10.3390/molecules27082516
Chicago/Turabian StyleLi, Yumei, Xinxin Zhang, Chunwen Lu, Peng Lu, Chongxu Yin, Zhengmao Ye, and Zhaosong Huang. 2022. "Identification and Characterization of a Novel Endo-β-1,4-Xylanase from Streptomyces sp. T7 and Its Application in Xylo-Oligosaccharide Production" Molecules 27, no. 8: 2516. https://doi.org/10.3390/molecules27082516