Effects of Regioisomerism on the Antiproliferative Activity of Hydroxystearic Acids on Human Cancer Cell Lines
Abstract
:1. Introduction
2. Results and Discussion
2.1. Synthesis of Regioisomeric Hydroxystearic Acids
2.2. Biological Activity
Cell Growth Inhibitory Effects of HSAs on Human Cancer Cell Lines
3. Materials and Methods
3.1. Chemical Synthesis
3.1.1. General
3.1.2. Synthesis of Methyl 5-Hydroxyoctadecanoate (4a)
3.1.3. Synthesis of 5-Hydroxyoctadecanoic Acid (5-HSA, 5a)
3.1.4. Synthesis of 11-Hydroxyoctadecanoic Acid (11-HSA, 5f)
Synthesis of 11-Hydroxy-9-octadecenoic Acid (8)
Synthesis of 11-Hydroxyoctadecanoic Acid (11-HSA, 5f)
3.2. Biology
3.2.1. Cell Culture and Treatments
3.2.2. MTT Assay
3.2.3. Quantitative Phase Image (QPI) Microscopy
3.3. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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5-HSA IC50 | 7-HSA IC50 | 8-HSA IC50 | 9-HSA IC50 | 10-HSA IC50 | 11HSA IC50 | |
---|---|---|---|---|---|---|
CaCo2 R square | 25.1 μM 0.8889 | 42.5 μM 0.9731 | > 100μM | 32.6 μM 0.9472 | 68.3 μM 0.9730 | 27.6 μM 0.9944 |
HT29 R square | 51.3 μM 0.9575 | 14.7 μM 0.9255 | > 100μM | 30.7 μM 0.9635 | 77.2 μM 0.9048 | 56.9 μM 0.8918 |
HeLa R square | 22.1 μM 0.9886 | 26.6 μM 0.9703 | > 100μM | 26.9 μM 0.9321 | 41.7 μM 0.8073 | 31.5 μM 0.8981 |
MCF7 R square | 46.4 μM 0.9820 | 21.4 μM 0.9447 | > 100μM | 49.1 μM 0.9866 | 38.7 μM 0.9599 | 35.8 μM 0.9681 |
PC3 R square | 31.6 μM 0.9516 | 24.3 μM 0.9183 | > 100μM | 23.4 μM 0.9499 | 34.0 μM 0.8826 | 61.4 μM 0.9610 |
NLF R square | 38.5 μM 0.9971 | 24.9 μM 0.8366 | > 100μM | 33.1 μM 0.9960 | 74.6 μM 0.9887 | 29.7 μM 0.9841 |
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Calonghi, N.; Boga, C.; Nitti, P.; Telese, D.; Bordoni, S.; Farruggia, G.; Asaro, F.; Grandi, M.; Zalambani, C.; Micheletti, G. Effects of Regioisomerism on the Antiproliferative Activity of Hydroxystearic Acids on Human Cancer Cell Lines. Molecules 2022, 27, 2396. https://doi.org/10.3390/molecules27082396
Calonghi N, Boga C, Nitti P, Telese D, Bordoni S, Farruggia G, Asaro F, Grandi M, Zalambani C, Micheletti G. Effects of Regioisomerism on the Antiproliferative Activity of Hydroxystearic Acids on Human Cancer Cell Lines. Molecules. 2022; 27(8):2396. https://doi.org/10.3390/molecules27082396
Chicago/Turabian StyleCalonghi, Natalia, Carla Boga, Patrizia Nitti, Dario Telese, Silvia Bordoni, Giovanna Farruggia, Fioretta Asaro, Martina Grandi, Chiara Zalambani, and Gabriele Micheletti. 2022. "Effects of Regioisomerism on the Antiproliferative Activity of Hydroxystearic Acids on Human Cancer Cell Lines" Molecules 27, no. 8: 2396. https://doi.org/10.3390/molecules27082396