Next Article in Journal
Identification of Stingless Bee Honey Adulteration Using Visible-Near Infrared Spectroscopy Combined with Aquaphotomics
Next Article in Special Issue
Powerful Antioxidants and Cytotoxic Activities of the Methanol Extracts from Eight Soybean Cultivars
Previous Article in Journal
Cytotoxicity of Callerya speciosa Fractions against Myeloma and Lymphoma Cell Lines
Previous Article in Special Issue
Antibacterial Activity of Chinese Red Propolis against Staphylococcus aureus and MRSA
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 27
Issue 7
10.3390/molecules27072323
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Aloe arborescens: In Vitro Screening of Genotoxicity, Effective Inhibition of Enzyme Characteristics for Disease Etiology, and Microbiological Activity

by
Kamil Pawłowicz
1,2,
Szymon Sip
2,
Tomasz Plech
3,
Barbara Kaproń
4,
Joanna Kobus-Cisowska
5 and
Judyta Cielecka-Piontek
2,*
1
Phytopharm Klęka S.A., Klęka 1, 63-040 Nowe Miasto nad Warta, Poland
2
Department of Pharmacognosy, Poznan University of Medical Sciences, Rokietnicka 3, 60-806 Poznan, Poland
3
Department of Pharmacology, Medical University of Lublin, Chodźki 4a, 20-093 Lublin, Poland
4
Department of Clinical Genetics, Medical University of Lublin, Radziwiłłowska 11, 20-080 Lublin, Poland
5
Department of Gastronomy Sciences and Functional Foods, Faculty of Food Science and Nutrition, Poznan University of Life Sciences (PULS), Wojska Polskiego Str. 28, 60-637 Poznan, Poland
*
Author to whom correspondence should be addressed.
Molecules 2022, 27(7), 2323; https://doi.org/10.3390/molecules27072323
Submission received: 27 February 2022 / Revised: 30 March 2022 / Accepted: 31 March 2022 / Published: 3 April 2022
(This article belongs to the Special Issue Characterization, Properties and Applications of Natural Products)
Browse Figures
Versions Notes

Abstract

The present study assessed the genotoxicity, the possibility of inhibiting selected enzymes, and the microbial activity of lyophilisate from 3-year-old A. arborescens leaves obtained from controlled crops. The lyophilisate from 3-year-old A. arborescens leaves was standardized for aloin A and aloenin A content. Moreover, concentrations of polyphenolic compounds and phenolic acids were determined. The first stage of the research was to determine genotoxicity using the comet test, which confirmed the safety of A. arborescens. Assays of enzymatic inhibition were performed for hyaluronidase (IC50 = 713.24 ± 41.79 µg/mL), α-glucosidase (IC50 = 598.35 ± 12.58 µg/mL), acetylcholinesterase and butyrylcholinesterase (1.16 vs. 0.34 µM of eserine/g d.m., respectively). The next stage of the research was to determine the ability of the healing properties using the scratch test, which showed a positive response using the extract. Microbial activity was evaluated and obtained against Gram-negative and Gram-positive bacteria and yeasts. We concluded that A. arborescens leaf gel meets the important conditions for plant raw materials to obtain semi-solid forms of herbal medicinal products.
Keywords: Aloe arborescens; in vitro safety; enzyme inhibitions; scratch test; microbiological activity Aloe arborescens; in vitro safety; enzyme inhibitions; scratch test; microbiological activity
Graphical Abstract

Share and Cite

MDPI and ACS Style

Pawłowicz, K.; Sip, S.; Plech, T.; Kaproń, B.; Kobus-Cisowska, J.; Cielecka-Piontek, J. Aloe arborescens: In Vitro Screening of Genotoxicity, Effective Inhibition of Enzyme Characteristics for Disease Etiology, and Microbiological Activity. Molecules 2022, 27, 2323. https://doi.org/10.3390/molecules27072323

AMA Style

Pawłowicz K, Sip S, Plech T, Kaproń B, Kobus-Cisowska J, Cielecka-Piontek J. Aloe arborescens: In Vitro Screening of Genotoxicity, Effective Inhibition of Enzyme Characteristics for Disease Etiology, and Microbiological Activity. Molecules. 2022; 27(7):2323. https://doi.org/10.3390/molecules27072323

Chicago/Turabian Style

Pawłowicz, Kamil, Szymon Sip, Tomasz Plech, Barbara Kaproń, Joanna Kobus-Cisowska, and Judyta Cielecka-Piontek. 2022. "Aloe arborescens: In Vitro Screening of Genotoxicity, Effective Inhibition of Enzyme Characteristics for Disease Etiology, and Microbiological Activity" Molecules 27, no. 7: 2323. https://doi.org/10.3390/molecules27072323

APA Style

Pawłowicz, K., Sip, S., Plech, T., Kaproń, B., Kobus-Cisowska, J., & Cielecka-Piontek, J. (2022). Aloe arborescens: In Vitro Screening of Genotoxicity, Effective Inhibition of Enzyme Characteristics for Disease Etiology, and Microbiological Activity. Molecules, 27(7), 2323. https://doi.org/10.3390/molecules27072323

Article Metrics

Back to TopTop