Anti-Inflammatory Dysidazirine Carboxylic Acid from the Marine Cyanobacterium Caldora sp. Collected from the Reefs of Fort Lauderdale, Florida †
Abstract
:1. Introduction
2. Results and Discussion
2.1. Isolation and Structure Elucidation
2.2. Identification of the Cyanobacterium
2.3. Biological Activity
3. Materials and Methods
3.1. General Experimental Procedures
3.2. Collection, Extraction and Isolation
3.3. Molecular Identification
3.4. Cancer Cell Viability Assay (HCT116)
3.5. NO Assay and Cell Viability Assay (RAW264.7 Cells)
3.6. iNOS mRNA Measurement in RAW264.7 Cells
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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C No | δC Mult. | δH (J in Hz) | COSY | HMBC a | δC Mult. | δH (J in Hz) |
---|---|---|---|---|---|---|
1 b | 2 c,d | |||||
1 | 176.2, C | 2 | 172.2, C | |||
2 | 29.4, CH | 2.49, s | 4 | 28.3, CH | 2.45, s | |
3 | 157.1, C | 2, 4, 5 | 156.6, C | |||
4 | 114.1, CH | 6.61, d (15.1) | 5, 6 | 2, 5 | 112.9, CH | 6.47, d (15.8) |
5 | 158.4, CH | 6.75, dt (15.1, 6.9) | 4, 6 | 6, 7 | 155.8, CH | 6.64, dt (15.8, 6.8) |
6 | 34.1, CH2 | 2.38, dt (6.9, 7.4) | 5, 7 | 4, 5, 7 | 33.2, CH2 | 2.28, dt (6.8, 7.5) |
7 | 29.0, CH2 | 1.52, m | 6, 8 | 5, 6 | 29.1, CH2 | 1.44, m |
8–15 | 30.3–30.8, CH2 | 1.35–1.24, m | 29.2–29.7, CH2 | 1.1–1.3, m | ||
16 | 33.0, CH2 | 1.32, m | 18 | 33.2, CH2 | 1.17, m | |
17 | 23.7, CH2 | 1.24, m | 18 | 18 | 22.6, CH2 | 1.17, m |
18 | 14.4, CH3 | 0.89, t (6.8) | 17 | 16, 17 | 14.1, CH3 | 0.79, t (6.8) |
19 | 52.2, CH3 | 3.34, s |
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Gunasekera, S.P.; Kokkaliari, S.; Ratnayake, R.; Sauvage, T.; dos Santos, L.A.H.; Luesch, H.; Paul, V.J. Anti-Inflammatory Dysidazirine Carboxylic Acid from the Marine Cyanobacterium Caldora sp. Collected from the Reefs of Fort Lauderdale, Florida. Molecules 2022, 27, 1717. https://doi.org/10.3390/molecules27051717
Gunasekera SP, Kokkaliari S, Ratnayake R, Sauvage T, dos Santos LAH, Luesch H, Paul VJ. Anti-Inflammatory Dysidazirine Carboxylic Acid from the Marine Cyanobacterium Caldora sp. Collected from the Reefs of Fort Lauderdale, Florida. Molecules. 2022; 27(5):1717. https://doi.org/10.3390/molecules27051717
Chicago/Turabian StyleGunasekera, Sarath P., Sofia Kokkaliari, Ranjala Ratnayake, Thomas Sauvage, Larissa A. H. dos Santos, Hendrik Luesch, and Valerie J. Paul. 2022. "Anti-Inflammatory Dysidazirine Carboxylic Acid from the Marine Cyanobacterium Caldora sp. Collected from the Reefs of Fort Lauderdale, Florida" Molecules 27, no. 5: 1717. https://doi.org/10.3390/molecules27051717