Ethyl Acetate Extract from Artemisia argyi Prevents Liver Damage in ConA-Induced Immunological Liver Injury Mice via Bax/Bcl-2 and TLR4/MyD88/NF-κB Signaling Pathways
Abstract
:1. Introduction
2. Results
2.1. LC-MS Analysis of AaEA
2.2. Effect of AaEA on ConA-Induced Liver Damage and Serum Transaminase Levels
2.3. Effects of AaEA on Liver Oxidative Stress
2.4. Effects of AaEA on IL-4, TNF-α, and IFN-γ
2.5. Histopathological Features of Mice Livers
2.6. Effects of AaEA on Liver Cell Apoptosis
2.7. AaEA Inhibited the Bax/Bcl-2 and TLR4/MyD88/NF-κB Signaling Pathways
3. Materials and Methods
3.1. Plant Material
3.2. Sample Preparation
3.3. LC-MS Analysis
3.4. Animals and Experimental Design
3.5. Serum Biomarker Assays
3.6. Oxidative Stress-Related Factors
3.7. Inflammatory Factors
3.8. Histopathological Analysis
3.9. TdT-Mediated dUTP Nick-End Labeling (TUNEL) Staining
3.10. Real-Time PCR Assay
3.11. Western Blotting Analysis
3.12. Statistical Analysis
4. Conclusions and Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Yang, W.; Shao, F.; Wang, J.; Shen, T.; Zhao, Y.; Fu, X.; Zhang, L.; Li, H. Ethyl Acetate Extract from Artemisia argyi Prevents Liver Damage in ConA-Induced Immunological Liver Injury Mice via Bax/Bcl-2 and TLR4/MyD88/NF-κB Signaling Pathways. Molecules 2022, 27, 7883. https://doi.org/10.3390/molecules27227883
Yang W, Shao F, Wang J, Shen T, Zhao Y, Fu X, Zhang L, Li H. Ethyl Acetate Extract from Artemisia argyi Prevents Liver Damage in ConA-Induced Immunological Liver Injury Mice via Bax/Bcl-2 and TLR4/MyD88/NF-κB Signaling Pathways. Molecules. 2022; 27(22):7883. https://doi.org/10.3390/molecules27227883
Chicago/Turabian StyleYang, Wenqian, Fei Shao, Jiexin Wang, Tong Shen, Yu Zhao, Xueyan Fu, Liming Zhang, and Hangying Li. 2022. "Ethyl Acetate Extract from Artemisia argyi Prevents Liver Damage in ConA-Induced Immunological Liver Injury Mice via Bax/Bcl-2 and TLR4/MyD88/NF-κB Signaling Pathways" Molecules 27, no. 22: 7883. https://doi.org/10.3390/molecules27227883
APA StyleYang, W., Shao, F., Wang, J., Shen, T., Zhao, Y., Fu, X., Zhang, L., & Li, H. (2022). Ethyl Acetate Extract from Artemisia argyi Prevents Liver Damage in ConA-Induced Immunological Liver Injury Mice via Bax/Bcl-2 and TLR4/MyD88/NF-κB Signaling Pathways. Molecules, 27(22), 7883. https://doi.org/10.3390/molecules27227883