DDX3 Upregulates Hydrogen Peroxide-Induced Melanogenesis in Sk-Mel-2 Human Melanoma Cells
Abstract
:1. Introduction
2. Results
2.1. H2O2 Activates Melanogenesis and DDX3 Promoter Activity
2.2. DDX3 Induces Melanogenesis in H2O2-Treated Condition Cells
2.3. DDX3 Binds to Tyrosinase in H2O2-Treated Condition
3. Discussion
4. Materials and Methods
4.1. Chemicals and Antibodies
4.2. Cell Culture
4.3. Melanin Content Measurement
4.4. Tyrosinase Activity Assay
4.5. Luciferase Reporter Assay
4.6. Immunoprecipitation (IP)
4.7. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Eom, S.; Lee, S.; Lee, J.; Yeom, H.D.; Lee, S.-G.; Lee, J. DDX3 Upregulates Hydrogen Peroxide-Induced Melanogenesis in Sk-Mel-2 Human Melanoma Cells. Molecules 2022, 27, 7010. https://doi.org/10.3390/molecules27207010
Eom S, Lee S, Lee J, Yeom HD, Lee S-G, Lee J. DDX3 Upregulates Hydrogen Peroxide-Induced Melanogenesis in Sk-Mel-2 Human Melanoma Cells. Molecules. 2022; 27(20):7010. https://doi.org/10.3390/molecules27207010
Chicago/Turabian StyleEom, Sanung, Shinhui Lee, Jiwon Lee, Hye Duck Yeom, Seong-Gene Lee, and Junho Lee. 2022. "DDX3 Upregulates Hydrogen Peroxide-Induced Melanogenesis in Sk-Mel-2 Human Melanoma Cells" Molecules 27, no. 20: 7010. https://doi.org/10.3390/molecules27207010