Interactions of Isoquinoline Alkaloids with Transition Metals Iron and Copper
Abstract
:1. Introduction
2. Results
2.1. Iron Chelation in Non-Buffered Conditions: All Tested Alkaloids Chelated iron
2.2. Ferrous Chelation under Different pH Conditions: Only Galantine and Glaucine Were Active at Slightly Acidic pH 6.8
2.3. Confirmation of the Ferric Chelation Effect of Glaucine in Non-Competitive Conditions
2.4. Copper Chelation: None of the Tested Alkaloids Significantly Chelated Cupric or Cuprous Ions
2.5. Metal Reduction: Most Alkaloids Reduced Ferric and, in Particular, Cupric Ions
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Iron/Copper Chelation-and-Reduction Determination
4.2.1. Reagent and Stock-Solution Preparation
4.2.2. Ferrozine Assay
4.2.3. Hematoxylin Assay
4.2.4. BCS Assay
4.2.5. Assessment of Iron Stoichiometry
Job’s Method
Complementary Method
4.2.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Parvin, M.S.; Chlebek, J.; Hošťálková, A.; Catapano, M.C.; Lomozová, Z.; Macáková, K.; Mladěnka, P. Interactions of Isoquinoline Alkaloids with Transition Metals Iron and Copper. Molecules 2022, 27, 6429. https://doi.org/10.3390/molecules27196429
Parvin MS, Chlebek J, Hošťálková A, Catapano MC, Lomozová Z, Macáková K, Mladěnka P. Interactions of Isoquinoline Alkaloids with Transition Metals Iron and Copper. Molecules. 2022; 27(19):6429. https://doi.org/10.3390/molecules27196429
Chicago/Turabian StyleParvin, Mst Shamima, Jakub Chlebek, Anna Hošťálková, Maria Carmen Catapano, Zuzana Lomozová, Kateřina Macáková, and Přemysl Mladěnka. 2022. "Interactions of Isoquinoline Alkaloids with Transition Metals Iron and Copper" Molecules 27, no. 19: 6429. https://doi.org/10.3390/molecules27196429