Cluster-Assembled Nanoporous Super-Hydrophilic Smart Surfaces for On-Target Capturing and Processing of Biological Samples for Multi-Dimensional MALDI-MS
Abstract
:1. Introduction
2. Material and Methods
2.1. Samples, Reagents and Procedures
2.2. Array-Patterned Ns-TiO2 Chips
2.3. In-Vial and On-Chip Comparison
2.4. Multi-Dimensional MALDI
2.5. Identification of Amino Acid Sequences in Poorly Soluble Amyloidogenic Fragments
2.6. Mass Spectrometry
3. Results and Discussion
3.1. Hydrophilic-Hydrophobic Containment Structures
3.2. Nanostructure Driven Matrix Crystallization
3.3. In-Vial vs. On-Chip Protein Digestion
3.4. Multi-Dimensional MALDI
3.5. Gelsolin Amyloid Peptide Detection
3.6. Multi-Dimensional Detection of Disulfide Bridges
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Barborini, E.; Bertolini, G.; Epifanio, M.; Yavorskyy, A.; Vinati, S.; Baumann, M. Cluster-Assembled Nanoporous Super-Hydrophilic Smart Surfaces for On-Target Capturing and Processing of Biological Samples for Multi-Dimensional MALDI-MS. Molecules 2022, 27, 4237. https://doi.org/10.3390/molecules27134237
Barborini E, Bertolini G, Epifanio M, Yavorskyy A, Vinati S, Baumann M. Cluster-Assembled Nanoporous Super-Hydrophilic Smart Surfaces for On-Target Capturing and Processing of Biological Samples for Multi-Dimensional MALDI-MS. Molecules. 2022; 27(13):4237. https://doi.org/10.3390/molecules27134237
Chicago/Turabian StyleBarborini, Emanuele, Giacomo Bertolini, Monica Epifanio, Alexander Yavorskyy, Simone Vinati, and Marc Baumann. 2022. "Cluster-Assembled Nanoporous Super-Hydrophilic Smart Surfaces for On-Target Capturing and Processing of Biological Samples for Multi-Dimensional MALDI-MS" Molecules 27, no. 13: 4237. https://doi.org/10.3390/molecules27134237