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Article

Magnetophotoselection in the Investigation of Excitonically Coupled Chromophores: The Case of the Water-Soluble Chlorophyll Protein

by
Susanna Ciuti
1,†,
Alessandro Agostini
1,2,†,
Antonio Barbon
1,
Marco Bortolus
1,
Harald Paulsen
3,
Marilena Di Valentin
1,* and
Donatella Carbonera
1,*
1
Department of Chemical Sciences, University of Padova, Via Marzolo 1, 35131 Padova, Italy
2
Biology Centre, Czech Academy of Sciences, Institute of Plant Molecular Biology, Branišovská 1160/31, 370 05 České Budějovice, Czech Republic
3
Institute of Molecular Physiology, Johannes Gutenberg-University of Mainz, Johann-Joachim Becher-Weg 7, 55128 Mainz, Germany
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Molecules 2022, 27(12), 3654; https://doi.org/10.3390/molecules27123654
Submission received: 5 May 2022 / Revised: 1 June 2022 / Accepted: 2 June 2022 / Published: 7 June 2022
(This article belongs to the Special Issue Application of EPR Spectroscopy in Biophysics and Biochemistry)
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Abstract

A magnetophotoselection (MPS) investigation of the photoexcited triplet state of chlorophyll a both in a frozen organic solvent and in a protein environment, provided by the water-soluble chlorophyll protein (WSCP) of Lepidium virginicum, is reported. The MPS experiment combines the photoselection achieved by exciting with linearly polarized light with the magnetic selection of electron paramagnetic resonance (EPR) spectroscopy, allowing the determination of the relative orientation of the optical transition dipole moment and the zero-field splitting tensor axes in both environments. We demonstrate the robustness of the proposed methodology for a quantitative description of the excitonic interactions among pigments. The orientation of the optical transition dipole moments determined by the EPR analysis in WSCP, identified as an appropriate model system, are in excellent agreement with those calculated in the point-dipole approximation. In addition, MPS provides information on the electronic properties of the triplet state, localized on a single chlorophyll a pigment of the protein cluster, in terms of orientation of the zero-field splitting tensor axes in the molecular frame.
Keywords: magnetophotoselection; triplet state; chlorophyll-binding protein; excitonic interaction; TR-EPR magnetophotoselection; triplet state; chlorophyll-binding protein; excitonic interaction; TR-EPR

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MDPI and ACS Style

Ciuti, S.; Agostini, A.; Barbon, A.; Bortolus, M.; Paulsen, H.; Di Valentin, M.; Carbonera, D. Magnetophotoselection in the Investigation of Excitonically Coupled Chromophores: The Case of the Water-Soluble Chlorophyll Protein. Molecules 2022, 27, 3654. https://doi.org/10.3390/molecules27123654

AMA Style

Ciuti S, Agostini A, Barbon A, Bortolus M, Paulsen H, Di Valentin M, Carbonera D. Magnetophotoselection in the Investigation of Excitonically Coupled Chromophores: The Case of the Water-Soluble Chlorophyll Protein. Molecules. 2022; 27(12):3654. https://doi.org/10.3390/molecules27123654

Chicago/Turabian Style

Ciuti, Susanna, Alessandro Agostini, Antonio Barbon, Marco Bortolus, Harald Paulsen, Marilena Di Valentin, and Donatella Carbonera. 2022. "Magnetophotoselection in the Investigation of Excitonically Coupled Chromophores: The Case of the Water-Soluble Chlorophyll Protein" Molecules 27, no. 12: 3654. https://doi.org/10.3390/molecules27123654

APA Style

Ciuti, S., Agostini, A., Barbon, A., Bortolus, M., Paulsen, H., Di Valentin, M., & Carbonera, D. (2022). Magnetophotoselection in the Investigation of Excitonically Coupled Chromophores: The Case of the Water-Soluble Chlorophyll Protein. Molecules, 27(12), 3654. https://doi.org/10.3390/molecules27123654

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