3.1. Effect of Processing Condition on Physical Properties of QBJR
The dried rice quality has been affected by the processing condition which caused the physical properties of the product such as rehydration and microstructure to change, and also caused the bioactive compound concentrations to vary. Song et al. [
3] stated that the microstructure of cooked rice during drying affected the rehydration properties, which were improved and resulted in a high quality of the final product. Therefore, the processing of QBJR, namely baking powder concentration, soaking temperature, and soaking time, on rehydration capacity, microstructure, and also the texture of final product were studied.
The rehydration capacity exhibited the capacity of the dried sample to absorb and retain the water in the sample. This is a one of many important parameters for dried samples, which depends on many factors. The results showed that baking powder concentration, soaking temperature, and soaking time has affected the rehydration capacity. At room temperature being used as the soaking temperature, the increased baking powder concentration increased the rehydration capacity, however, the baking powder concentration of 0.3 and 0.5% was not different. This was in accordance with Song et al. [
3] who stated that the higher concentration of baking powder used, the higher the rehydration ratio of rice. This can be explained by the gas production of baking powder causing the space in the final QBJR structure after the drying process, which improves the rehydration capacity of the samples. Moreover, the rehydration capacity of the sample was not differed at baking powder concentrations of 0.3% at a soaking temperature of room temperature and that of 50 °C. However, at the soaking temperature of 60 °C, the increased baking powder concentration did not affect the rehydration capacity of QBJR samples. This was probably due to a high concentration; the generated gas may be evaporated during soaking processing.
Considering the effect of soaking time, the results exhibited that the longer soaking time (30 min) caused the decrease of rehydration capacity especially at high soaking temperature, but no effect found for a soaking time of 10 and 20 min. This probably can be explained by the maximum swelling of starch granules at high temperatures, and probably the breakdown of starch granules, which may affect the capacity of the samples to retain the absorbed water during the rehydration process. Moreover, at high soaking temperatures, the starch or soluble material would be subjected to increased leaching from the kernel [
17]. This would lead to the lower rehydration capacity of samples at a high soaking temperature for a longer time.
To explain the processing factors on the rehydration capacity of rice samples, the morphology of the samples was observed. Obviously, the morphology of the samples was not affected by the soaking time. Therefore, the morphology is shown for the same soaking time of 10 min (
Figure 2). The results revealed that the morphology of the samples, which soaked at 50 and 60 °C, had large amounts of the small pore structure, a honeycomb pattern, especially at soaking temperature of 60 °C, and also some large-pore structures. This appearance was also found in research work conducted by Zui et al. [
5] who found that for the rice soaked at 50 −70 °C, the honeycomb pattern was observed and some cracks were also found. This can be explained by the loosening of granules and the swelling of the starch granules. This would be in agreement with the more-swelled starch granules, and may be some broken down starch granules at a high temperature of soaking. On the contrary, for the morphology of the samples which soaked at room temperature, the large pore structure was observed and we did not observe the small pore structure. This large pore structure seems to affect the high rehydration capacity. It can be pointed out that the processing condition caused the difference in sample morphology which is in accordance with Zui et al. [
5].
The texture properties of rehydrated samples including hardness and springiness is also one important factor for studying the eating quality of cooked rice. The bite resistance of samples refers to their hardness value, meaning a firm structure of rice. The results (
Figure 3) exhibited that at a low soaking temperature and increased concentrations of baking powder, the hardness was decreased. This was related to the rehydration capacity, for which lower hydration resulting in a high hardness value. Moreover, at high soaking temperatures the hardness of samples was higher than at low soaking temperatures for every soaking time. This would be the changes of the starch granules which could be broken down during the soaking procedure. Consequently, the sample could not store the absorbed water hence the grain has high hardness. Concerning the springiness of the sample, which refers to the deforming of the clump in the mouth, it is found that with the increase of baking powder concentration from 0.1 to 0.3%, the springiness was increased, but the springiness of the samples soaking at 0.1% of baking powder concentration was similar to the 0.5% concentration. Moreover, the effect of soaking temperature and time did not affect the springiness. Song et al. [
3] found that when they compared the quick rice sample that was soaked in baking powder to the unsoaked sample, the springiness value was lower in the latter sample. This also pointed out that the gas production from soaking in baking powder solution would improve the springiness. From the results, it showed that the baking powder concentration of 0.3% gained the highest values of hardness and springiness.
3.2. Effect of Processing Condition on Bioactive Compound Content
The black rice is rich in nutrients and also bioactive components, for example, essential amino acids, lipids, dietary fiber, vitamins (B complex, A and E), some minerals (K, Fe, Zn, Cu, Mg, Mn, and P), anthocyanins, and phenolic compounds [
6]. This bioactive compound can be destroyed during the rice processing. In addition, the effect of quick rice production on bioactive compound concentrations has not been demonstrated yet. Therefore, the bioactive compound of QBJR were determined and discussed.
This research has studied three processing factors on the total phenolic (TPC), total flavonoid (TFC), as well as total anthocyanin (TAC) content as shown in
Figure 4. The results exhibited that the higher baking powder concentration and soaking temperature for a longer time declined the bioactive compound content in QBJR. This can be explained by the pH-sensitive bioactive compound, for which the baking powder conferred a higher pH to the soaking water and resulted in the unstable phenolic, flavonoid, and anthocyanin contents in the QBJR. Moreover, these compounds are heat-sensitive and water soluble, hence the high soaking temperature and longer soaking time resulted in the destroying and leaching of all bioactive compound to the soaking water.
Phenolic compounds, divided into two groups, consists of (1) flavonoids and phenolic acids and (2) coumarins, for which their structures contain one or more hydroxyl groups on an aromatic ring [
6]. The flavonoids in rice grains have been reported to be O- or C-glycosides [
7]. The phenolic compounds were unstable also in the high-pH environment. Friedman and Jürgens [
18] reported that polyphenolic compounds are damaged when exposed to a high pH. Moreover, the thermal processing also affect the phenolic composition by chemical and physical reactions. Duodu [
19] stated that during thermal processing the matrix-bound phenolics can be released, polymerized, and/or be thermally degraded. Lang, et al. [
20] reported that during different drying temperature of black rice, the free phenolic compounds can be complexed with some structural components, such as proteins or fibers at drying temperature of 60 and 80 °C and phenolics can be degraded at a drying temperature of 100 °C.
Moreover, the black rice also contained a high amount of anthocyanin, which is mainly found as cyanidin-3-glucoside, Cy3G [
9]. Anthocyanin is a water-soluble natural food pigment and the stability of anthocyanin depends on its structure, in which petunidin or malvidin aglycones are more stable than pelargonidin, cyanidin, or delphinidin aglycones [
8]. Moreover, the increase in glycosylation will improve its stability [
8]. Anthocyanins can be degraded with an alkaline pH. Sui, Ding and Zhou [
21] stated that they were increasingly degraded at the pH level of above 6.0 and under thermal treatment. Cabrita, Fossen, and Andersen [
22] reported that cyanidin3-glucoside was highly degraded at a pH about 5.0–6.0. In our study, the baking powder solution was found to have a pH of about 7.0; this pH values would affect the anthocyanin content. However, the objective of this work was to study the best soaking conditions to produce the Quick Black Jasmine Rice in order to make it easier and faster to cook, and from our results it showed that the final product retained the high bioactive compound.
Moreover, one of the main factors that reduces the stability of Cy3G is thermal processing [
23]. Dong et al. [
23] reported that from thermogravimetric analysis showed that the Cy3Gs are thermally degraded in three steps, in which the first step occurred at 120–210 °C. Moreover, they also stated that the anthocyanin-rich foods could be conventionally cooked, for example through steaming, boiling, and microwave heating, in which the cooking temperatures would be reached about 50–150 °C. Therefore, in our experiment the soaking temperature did not destroy the anthocyanin, especially Cy3G, even though some anthocyanin compound may be transformed. However, to look into this more deeply, the anthocyanin profile of the final sample could be determined.
Even though the soaking process could improve the physical properties of QBJR, however, some bioactive compounds would be unstable during processing. Therefore, this point could be concerned in order to produce the quick rice product especially for black rice, which are rich in bioactive compounds.
3.3. Effect of Processing Condition on Sensory Evaluation
The sensory characteristics of the QBJR were evaluated by 30 panelists, Thai students who studied in Bachelor of Science Program in Food Technology and Innovation, Faculty of Interdisciplinary Studies, Khon Kaen University, Thailand, for three attributes: softener, flavor, and overall acceptance. The sensory score of the samples is shown in
Figure 5. The three processing variables were evaluated on terms of their effects on the sensory score of the final samples. The results showed that the concentration of baking powder influenced the flavor score, in which for higher concentrations, the flavor score was increased. Even though the baking powder would have some aftertaste effect, however, in our study we found that the panelists did not seem too unappreciative of the flavor of the final sample which soaked at a high baking powder concentration. Concerning the softener score, the results exhibited that the baking powder concentration, soaking temperature, as well as the soaking time affected the softener score of samples. The increase of baking powder concentration resulted in an increase of softener score especially at a low soaking temperature; however at high temperatures, the concentrations of 0.3 and 0.5% was not differ. This result was in accordance with the rehydration capacity of the sample in which for the high rehydration capacity, the high softener score was observed. Furthermore, the soaking time at a low temperature influenced the softener score, in which for the longer soaking time, a higher softener score was found. These were in agreement with the hardness value delivered from the texture analyzer profile. In addition, the overall acceptance score of QBJR samples increased when higher baking powder concentrations were used. Meanwhile, for the longer soaking time, the overall acceptance score was increased. This result was in agreement with hardness values as well as the hardness score of QBJR samples. Consequently, all sensory evaluation data demonstrated that the soaking procedure could affect the sensory score and the physical properties of the samples, which were also related to the sensory score.
The evaluation for the best condition for QBJR production concerning the physical properties, sensory characteristics, as well as the amounts of bioactive compounds were performed by the Fuzzy analytical method. This method is discussed in the following section.
3.4. The Evaluation the Best Processing Condition
The FAM is a mathematical technique and has been performed for decision making. This method transfers all data to a fuzzy scale, fuzzy grade, and to the overall performance index. Moreover, the application of FAM on more than seven criteria for which each criterion contains unequal data has never been reported before. In this work, the main criterion was divided into three criteria, namely physical properties, bioactive compound content, and sensory score with the wight of 30:40:30, respectively. In the physical property criterion it was separated into three sub-criteria, which were the rehydration capacity, hardness values, and springiness values. Conferring these criteria to the overall physical properties, the weight of each sub-criteria was 15, 10, and 5 for the rehydration capacity, hardness values, and springiness values, respectively. Considering the importance and specificity of bioactive compounds in black rice, the total anthocyanin content was the main sub-criteria of this criteria with the weight of 20, while the weight of the total phenolic content and total flavonoid content were equal, being 10. For the sensory score data, the sub-criteria were flavor, softener, and overall acceptance score with the same weight. Furthermore, in each QBJR sample, the data of each sub-criteria in terms of physical properties as well as bioactive compound criteria consisted of three sets of experimental data, while the data of sensory score criteria consisted of 30 sets of experimental data. According to each sub-criterion, it consisted of unequal data, therefore all 108 pieces of data of each sample were translated into the fuzzy scale that was designed (0, 10), for which the number 10 has been determined as perfect performance, as the following equation:
For all data
, where
is the maximum and
is the minimum of each criterion from all sample.. Except for hardness criteria, which has to be transferred in reverse, in which the maximum scale is not perfect, the fuzzy scale of the hardness criteria can be translated as the following equation:
So, each data in the different boundary were transformed in the same range [0, 10], for which this range was used to define the fuzzy performance grade sets by using the triangular fuzzy numbers, as seen in
Figure 7.
Next, the fuzzy score of each sub-criteria were calculated into the fuzzy performance grade sets
A,
B,…,
F (ranging from the best to the worst), which was then used to integrate with each relative weight, which was called the index score are is denoted by
. Then, all 108 index scores were combined to calculate the overall index. Therefore, the obtained overall index was calculated from the same fuzzy performance grade set. The overall index of each sample (
Figure 6) exhibited that the soaking in 0.1% of baking powder at room temperature for 30 min was the best soaking condition for producing the QBJR. This condition showed high physical quality with a high amount of bioactive compounds, as well as a high sensory score. This shows that FAM can be used as an efficiency evaluation method for the discission of the best condition in this present work, especially when many criterions and sub-criterions were concerned. Moreover, it is an effective tool for unequal data and also some data have to be considered in a reverse response.