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Article

Determination of 18 Intact Glucosinolates in Brassicaceae Vegetables by UHPLC-MS/MS: Comparing Tissue Disruption Methods for Sample Preparation

by
Xiaolu Yu
,
Hongju He
,
Xuezhi Zhao
,
Guangmin Liu
,
Liping Hu
,
Bing Cheng
and
Yaqin Wang
*
Institute of Agri-Food Processing and Nutrition, Beijing Vegetable Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
*
Author to whom correspondence should be addressed.
Molecules 2022, 27(1), 231; https://doi.org/10.3390/molecules27010231
Submission received: 29 November 2021 / Revised: 22 December 2021 / Accepted: 28 December 2021 / Published: 30 December 2021
(This article belongs to the Section Analytical Chemistry)
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Abstract

Glucosinolates (GSLs) are important precursor compounds with anticancer activities in Brassicaceae vegetables and are readily hydrolyzed by myrosinase. Given the diversity of these species, establishing an accurate and universal method to quantify intact GSLs in different plant tissues is necessary. Here, we compared and optimized three tissue disruption methods for sample preparation. After microwave treatment for 90 s, 13 GSLs in homogenized Chinese cabbage samples were recovered at 73–124%. However, a limitation of this method was that different tissues could not be processed under the same microwave conditions. Regarding universality, GSLs in Brassicaceae vegetables could be extracted from freeze-dried sample powder with 70% methanol (v/v) or frozen-fresh sample powder with 80% methanol (v/v). Moreover, heating extraction is necessary for GSLs extracted from frozen-fresh sample powder. Average recoveries of the two optimized methods were 74–119% with relative standard deviations ≤ 15%, with the limits of quantification 5.72–17.40 nmol/g dry weight and 0.80–1.43 nmol/g fresh weight, respectively. Notably, the method for analyzing intact GSLs was more efficient than that for desulfo-GSLs regarding operational complexity, detection speed and quantification accuracy. The developed method was applied to identify the characteristic GSLs in 15 Brassicaceae vegetables, providing a foundation for further research on GSLs.
Keywords: intact glucosinolates; Brassicaceae vegetables; triple quadrupole mass spectrometry; tissue disruption intact glucosinolates; Brassicaceae vegetables; triple quadrupole mass spectrometry; tissue disruption
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MDPI and ACS Style

Yu, X.; He, H.; Zhao, X.; Liu, G.; Hu, L.; Cheng, B.; Wang, Y. Determination of 18 Intact Glucosinolates in Brassicaceae Vegetables by UHPLC-MS/MS: Comparing Tissue Disruption Methods for Sample Preparation. Molecules 2022, 27, 231. https://doi.org/10.3390/molecules27010231

AMA Style

Yu X, He H, Zhao X, Liu G, Hu L, Cheng B, Wang Y. Determination of 18 Intact Glucosinolates in Brassicaceae Vegetables by UHPLC-MS/MS: Comparing Tissue Disruption Methods for Sample Preparation. Molecules. 2022; 27(1):231. https://doi.org/10.3390/molecules27010231

Chicago/Turabian Style

Yu, Xiaolu, Hongju He, Xuezhi Zhao, Guangmin Liu, Liping Hu, Bing Cheng, and Yaqin Wang. 2022. "Determination of 18 Intact Glucosinolates in Brassicaceae Vegetables by UHPLC-MS/MS: Comparing Tissue Disruption Methods for Sample Preparation" Molecules 27, no. 1: 231. https://doi.org/10.3390/molecules27010231

APA Style

Yu, X., He, H., Zhao, X., Liu, G., Hu, L., Cheng, B., & Wang, Y. (2022). Determination of 18 Intact Glucosinolates in Brassicaceae Vegetables by UHPLC-MS/MS: Comparing Tissue Disruption Methods for Sample Preparation. Molecules, 27(1), 231. https://doi.org/10.3390/molecules27010231

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