Antioxidant Assessment of Prenylated Stilbenoid-Rich Extracts from Elicited Hairy Root Cultures of Three Cultivars of Peanut (Arachis hypogaea)
Abstract
:1. Introduction
2. Results and Discussion
2.1. Development and Characterization of Peanut cv. Tifrunner Hairy Roots
2.2. Production of Prenylated Stilbenoids in Hairy Roots of Peanut cvs. Tifrunner, Hull, and Georgia Green
2.3. Comparison of Antioxidant Activity of Stilbenoid-Rich Extract from Hairy Roots of Three Peanut Cultivars
3. Materials and Methods
3.1. Seed Sterilization and Germination of Peanut cv. Tifrunner
3.2. Establishment of Hairy Root Cultures of Peanut cv. Tifrunner
3.3. Growth Conditions and Elicitation of Peanut Hairy Root Cultures of cvs. Tifrunner, Hull, and Georgia Green
3.4. Extraction and Analysis of Stilbenoids
3.5. DPPH Antioxidant Assay
3.6. Purification and Identification of Arachidin-6 in Peanut Hairy Root Culture
3.7. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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tR (Min) | UV Max (nm) | [M − H]− | MS2 Ions a | MS3 Ions | [M + H]+ | MS2 Ions a | MS3 Ions |
---|---|---|---|---|---|---|---|
16.57 | 344 | 309 | 291, 265, 294 | 159, 249 | 311 | 201, 283, 296 | 159, 173, 183 |
Stilbenoids | µg/mg DW a | ||
---|---|---|---|
Tifrunner | Hull | Georgia Green | |
Resveratrol | 29.47 ± 1.40 | 60.56 ± 1.19 | 12.52 ± 0.29 |
Arachidin-5 | 30.92 ± 1.52 | 13.5 ± 0.29 | 6.9 ± 0.13 |
Arachidin-1 | 207.5 ± 7.35 | 162.37 ± 1.33 | 108.76 ± 1.53 |
Arachidin-2 | 39.15 ± 0.98 | 28.21 ± 1.97 | 19.74 ± 2.21 |
Arachidin-3 | 75.28 ± 7.39 | 72.24 ± 2.05 | 46.78 ± 0.52 |
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Gajurel, G.; Hasan, R.; Medina-Bolivar, F. Antioxidant Assessment of Prenylated Stilbenoid-Rich Extracts from Elicited Hairy Root Cultures of Three Cultivars of Peanut (Arachis hypogaea). Molecules 2021, 26, 6778. https://doi.org/10.3390/molecules26226778
Gajurel G, Hasan R, Medina-Bolivar F. Antioxidant Assessment of Prenylated Stilbenoid-Rich Extracts from Elicited Hairy Root Cultures of Three Cultivars of Peanut (Arachis hypogaea). Molecules. 2021; 26(22):6778. https://doi.org/10.3390/molecules26226778
Chicago/Turabian StyleGajurel, Gaurav, Rokib Hasan, and Fabricio Medina-Bolivar. 2021. "Antioxidant Assessment of Prenylated Stilbenoid-Rich Extracts from Elicited Hairy Root Cultures of Three Cultivars of Peanut (Arachis hypogaea)" Molecules 26, no. 22: 6778. https://doi.org/10.3390/molecules26226778