Next Article in Journal
Chloroquine Potentiates the Anticancer Effect of Pterostilbene on Pancreatic Cancer by Inhibiting Autophagy and Downregulating the RAGE/STAT3 Pathway
Next Article in Special Issue
Semi-Quantitative MALDI Measurements of Blood-Based Samples for Molecular Diagnostics
Previous Article in Journal
Finding the First Potential Inhibitors of Shikimate Kinase from Methicillin Resistant Staphylococcus aureus through Computer-Assisted Drug Design
 
 
Article

Proteomic and Bioinformatic Analysis of Decellularized Pancreatic Extracellular Matrices

1
Department of Biomedical Engineering, Thomas J. Watson College of Engineering and Applied Sciences, Binghamton University, Binghamton, NY 13902, USA
2
Department of Pharmaceutical Sciences, School of Pharmacy and Pharmaceutical Sciences, Binghamton University, Binghamton, NY 13902, USA
3
Center of Biomanufacturing for Regenerative Medicine, Binghamton University, Binghamton, NY 13902, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Fulvio Magni, Marco Gaspari and Isabella Piga
Molecules 2021, 26(21), 6740; https://doi.org/10.3390/molecules26216740
Received: 4 September 2021 / Revised: 4 November 2021 / Accepted: 6 November 2021 / Published: 8 November 2021
Tissue microenvironments are rich in signaling molecules. However, factors in the tissue matrix that can serve as tissue-specific cues for engineering pancreatic tissues have not been thoroughly identified. In this study, we performed a comprehensive proteomic analysis of porcine decellularized pancreatic extracellular matrix (dpECM). By profiling dpECM collected from subjects of different ages and genders, we showed that the detergent-free decellularization method developed in this study permits the preservation of approximately 62.4% more proteins than a detergent-based method. In addition, we demonstrated that dpECM prepared from young pigs contained approximately 68.5% more extracellular matrix proteins than those prepared from adult pigs. Furthermore, we categorized dpECM proteins by biological process, molecular function, and cellular component through gene ontology analysis. Our study results also suggested that the protein composition of dpECM is significantly different between male and female animals while a KEGG enrichment pathway analysis revealed that dpECM protein profiling varies significantly depending on age. This study provides the proteome of pancreatic decellularized ECM in different animal ages and genders, which will help identify the bioactive molecules that are pivotal in creating tissue-specific cues for engineering tissues in vitro. View Full-Text
Keywords: pancreatic extracellular matrix proteins; proteomics; bioinformatics; decellularization; age and gender; KEGG pathway pancreatic extracellular matrix proteins; proteomics; bioinformatics; decellularization; age and gender; KEGG pathway
Show Figures

Figure 1

MDPI and ACS Style

Hu, M.; Bi, H.; Moffat, D.; Blystone, M.; DeCostanza, P.; Alayi, T.; Ye, K.; Hathout, Y.; Jin, S. Proteomic and Bioinformatic Analysis of Decellularized Pancreatic Extracellular Matrices. Molecules 2021, 26, 6740. https://doi.org/10.3390/molecules26216740

AMA Style

Hu M, Bi H, Moffat D, Blystone M, DeCostanza P, Alayi T, Ye K, Hathout Y, Jin S. Proteomic and Bioinformatic Analysis of Decellularized Pancreatic Extracellular Matrices. Molecules. 2021; 26(21):6740. https://doi.org/10.3390/molecules26216740

Chicago/Turabian Style

Hu, Ming, Huanjing Bi, Deana Moffat, Margaret Blystone, Paul DeCostanza, Tchilabalo Alayi, Kaiming Ye, Yetrib Hathout, and Sha Jin. 2021. "Proteomic and Bioinformatic Analysis of Decellularized Pancreatic Extracellular Matrices" Molecules 26, no. 21: 6740. https://doi.org/10.3390/molecules26216740

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop