Analysis of Flavonoid Metabolites in Watercress (Nasturtium officinale R. Br.) and the Non-Heading Chinese Cabbage (Brassica rapa ssp. chinensis cv. Aijiaohuang) Using UHPLC-ESI-MS/MS
Abstract
:1. Introduction
2. Materials and Methods
2.1. Experimental Materials and Methods
2.2. Determination of Total Flavonoids Content
2.3. Extraction Process
2.4. Chromatographic and Mass Spectrometry of Analysis Conditions
2.5. Qualitative and Quantitative Principles of Metabolites
2.6. Statistical Analysis
3. Results
3.1. Determination of Total Flavonoid Content
3.2. Qualitative and Quantitative Analyses of Metabolites and Quality Control (QC) Analysis of Samples
3.3. Principal Component Analysis (PCA) for the Different Varieties
3.4. Orthogonal Projections to Latent Structure-Discriminant Analysis (OPLS-DA)
3.5. Screening and Kegg Analysis of Flavonoid Differential Metabolites
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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UHPLC Conditions | Parameters | |
---|---|---|
The chromatographic columns | Agilent SB-C18 1.8 µm, 2.1 mm × 100 mm | |
Mobile phase | Phase A: ultra-pure water (0.1% formic acid was added) phase B: acetonitrile (0.1% formic acid was added) | |
Gradient program | 0 min | 95:5 (v:v) |
9.0 min | 5:95 (v:v) | |
10.0 min | 5:95 (v:v) | |
11.0 min | 95:5 (v:v) | |
14.0 min | 95:5 (v:v) | |
Flow rate | 0.40 mL/min | |
Temperature | 40 °C | |
Injection volume | 5 μL |
Mass Spectrometry Condition | Parameters | |
---|---|---|
Ion source | Turbo spray | |
Source temperature | 550 °C | |
Ion spray voltage | 5500 V | |
Ion source | Gas I | 55 psi |
Gas II | 60 psi | |
Curtain gas | 25 psi | |
Collision gas | High |
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Ma, X.; Ding, Q.; Hou, X.; You, X. Analysis of Flavonoid Metabolites in Watercress (Nasturtium officinale R. Br.) and the Non-Heading Chinese Cabbage (Brassica rapa ssp. chinensis cv. Aijiaohuang) Using UHPLC-ESI-MS/MS. Molecules 2021, 26, 5825. https://doi.org/10.3390/molecules26195825
Ma X, Ding Q, Hou X, You X. Analysis of Flavonoid Metabolites in Watercress (Nasturtium officinale R. Br.) and the Non-Heading Chinese Cabbage (Brassica rapa ssp. chinensis cv. Aijiaohuang) Using UHPLC-ESI-MS/MS. Molecules. 2021; 26(19):5825. https://doi.org/10.3390/molecules26195825
Chicago/Turabian StyleMa, Xiaoqing, Qiang Ding, Xilin Hou, and Xiong You. 2021. "Analysis of Flavonoid Metabolites in Watercress (Nasturtium officinale R. Br.) and the Non-Heading Chinese Cabbage (Brassica rapa ssp. chinensis cv. Aijiaohuang) Using UHPLC-ESI-MS/MS" Molecules 26, no. 19: 5825. https://doi.org/10.3390/molecules26195825