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Article

Supercritical Carbon Dioxide Extraction of Four Medicinal Mediterranean Plants: Investigation of Chemical Composition and Antioxidant Activity

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Laboratory for Aquaculture Biotechnology, Division of Materials Chemistry, Ruđer Bošković Institute, Bijenička 54, 10000 Zagreb, Croatia
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Center of Excellence for Marine Bioprospecting (BioProCro), Ruđer Bošković Institute, Bijenička 54, 10000 Zagreb, Croatia
3
Faculty of Health Sciences, University of Ljubljana, Zdravstvena pot 5, 1000 Ljubljana, Slovenia
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Chemistry Department, Lomonosov Moscow State University, Leninskie Gory 1/3, 119991 Moscow, Russia
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Core Facility Center “Arktika”, Lomonosov Northern (Arctic) Federal University, nab. Severnoy Dviny 17, 163002 Arkhangelsk, Russia
6
Škrlj, d.o.o., Batuje 90, 5262 Črniče, Slovenia
*
Author to whom correspondence should be addressed.
Academic Editor: Pedro Lozano Rodríguez
Molecules 2021, 26(18), 5697; https://doi.org/10.3390/molecules26185697
Received: 27 August 2021 / Revised: 14 September 2021 / Accepted: 16 September 2021 / Published: 20 September 2021
(This article belongs to the Special Issue Isolation and Characterization of Antioxidants from Natural Sources)
With everyday advances in the field of pharmaceuticals, medicinal plants have high priority regarding the introduction of novel synthetic compounds by the usage of environmentally friendly extraction technologies. Herein, a supercritical CO2 extraction method was implemented in the analysis of four plants (chamomile, St. John’s wort, yarrow, and curry plant) after which the non-targeted analysis of the chemical composition, phenolic content, and antioxidant activity was evaluated. The extraction yield was the highest for the chamomile (5%), while moderate yields were obtained for the other three plants. The chemical composition analyzed by gas chromatography-high-resolution mass spectrometry (GC-HRMS) and liquid chromatography-high-resolution mass spectrometry (LC-HRMS) demonstrated extraction of diverse compounds including terpenes and terpenoids, fatty acids, flavonoids and coumarins, functionalized phytosterols, and polyphenols. Voltammetry of microfilm immobilized on a glassy carbon electrode using square-wave voltammetry (SWV) was applied in the analysis of extracts. It was found that antioxidant activity obtained by SWV correlates well to 1,1-diphenyl-2-picrylhidrazine (DPPH) radical assay (R2 = 0.818) and ferric reducing antioxidant power (FRAP) assay (R2 = 0.640), but not to the total phenolic content (R2 = 0.092). Effective results were obtained in terms of activity showing the potential usage of supercritical CO2 extraction to acquire bioactive compounds of interest. View Full-Text
Keywords: green solvents; chamomile; yarrow; St. John’s wort; curry plant; square-wave voltammetry; chromatography green solvents; chamomile; yarrow; St. John’s wort; curry plant; square-wave voltammetry; chromatography
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MDPI and ACS Style

Čižmek, L.; Bavcon Kralj, M.; Čož-Rakovac, R.; Mazur, D.; Ul’yanovskii, N.; Likon, M.; Trebše, P. Supercritical Carbon Dioxide Extraction of Four Medicinal Mediterranean Plants: Investigation of Chemical Composition and Antioxidant Activity. Molecules 2021, 26, 5697. https://doi.org/10.3390/molecules26185697

AMA Style

Čižmek L, Bavcon Kralj M, Čož-Rakovac R, Mazur D, Ul’yanovskii N, Likon M, Trebše P. Supercritical Carbon Dioxide Extraction of Four Medicinal Mediterranean Plants: Investigation of Chemical Composition and Antioxidant Activity. Molecules. 2021; 26(18):5697. https://doi.org/10.3390/molecules26185697

Chicago/Turabian Style

Čižmek, Lara, Mojca Bavcon Kralj, Rozelindra Čož-Rakovac, Dmitrii Mazur, Nikolay Ul’yanovskii, Marko Likon, and Polonca Trebše. 2021. "Supercritical Carbon Dioxide Extraction of Four Medicinal Mediterranean Plants: Investigation of Chemical Composition and Antioxidant Activity" Molecules 26, no. 18: 5697. https://doi.org/10.3390/molecules26185697

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