Effects of Vanadyl Complexes with Acetylacetonate Derivatives on Non-Tumor and Tumor Cell Lines
Abstract
:1. Introduction
2. Results
2.1. Intracellular Localization of Vanadyl Complexes
2.2. Effect of Vanadyl Complexes on Cell Cycle
2.3. Effect of Vanadyl Complexes on MAPK
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Synthesis of Ligands and Complexes
4.3. Cell Culture
4.4. Confocal Microscopy
4.5. Cell Cycle
4.6. Western Blot Analysis
4.7. Cell Viability
4.8. Statistics
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Complex c | + AZD6244 | + SB202190 | + SP600125 | |
hTERT-HME1 | 5.65 ± 1.56 | 6.58 ± 1.12 | 6.58 ± 1.12 | 5.20 ± 1.14 |
Podocytes | 5.49 ± 1.07 | 20.16 ± 1.33 *** | 44.29 ± 1.54 *** | 6.03 ± 1.04 |
HCT 116 | 9.62 ± 1.07 | 13.31 ± 1.15 | 13.06 ± 1.09 | 10.34 ± 1.13 |
HT-29 | 18.91 ± 1.02 | 19.87 ± 1.02 | 22.64 ± 1.23 | 19.53 ± 1.31 |
Complex d | + AZD6244 | + SB202190 | + SP600125 | |
hTERT-HME1 | 3.53 ± 1.01 | 5.21 ± 1.89 | 4.71 ± 1.06 | 5.33 ± 1.59 |
Podocytes | 5.16 ± 1.10 | 6.25 ± 1.24 | 4.14 ± 1.11 | 5.03 ± 1.06 |
HCT 116 | 4.42 ± 1.07 | 6.81 ± 1.14 * | 7.99 ± 1.12 ** | 5.74 ± 1.09 |
HT-29 | 16.74 ± 1.95 | 11.98 ± 1.05 | 25.99 ± 1.45 | 19.20 ± 1.20 |
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Boscaro, V.; Barge, A.; Deagostino, A.; Ghibaudi, E.; Laurenti, E.; Marabello, D.; Diana, E.; Gallicchio, M. Effects of Vanadyl Complexes with Acetylacetonate Derivatives on Non-Tumor and Tumor Cell Lines. Molecules 2021, 26, 5534. https://doi.org/10.3390/molecules26185534
Boscaro V, Barge A, Deagostino A, Ghibaudi E, Laurenti E, Marabello D, Diana E, Gallicchio M. Effects of Vanadyl Complexes with Acetylacetonate Derivatives on Non-Tumor and Tumor Cell Lines. Molecules. 2021; 26(18):5534. https://doi.org/10.3390/molecules26185534
Chicago/Turabian StyleBoscaro, Valentina, Alessandro Barge, Annamaria Deagostino, Elena Ghibaudi, Enzo Laurenti, Domenica Marabello, Eliano Diana, and Margherita Gallicchio. 2021. "Effects of Vanadyl Complexes with Acetylacetonate Derivatives on Non-Tumor and Tumor Cell Lines" Molecules 26, no. 18: 5534. https://doi.org/10.3390/molecules26185534