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Article

Identification of the Primary Structure of Selenium-Containing Polysaccharides Selectively Inhibiting T-Cell Proliferation

by
Marzenna Klimaszewska
1,†,
Sabina Górska
2,†,
Grzegorz Łapienis
3,
Beata Kaleta
4,
Sandra Górska
1,
Marta Kaszowska
5,
Maciej Dawidowski
1,
Andrzej Gamian
6,
Radoslaw Zagożdżon
4,
Andrzej Górski
7 and
Jadwiga Turło
1,*
1
Department of Drug Technology and Pharmaceutical Biotechnology, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland
2
Microbiome Immunobiology Laboratory, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wroclaw, Poland
3
Division of Polymers, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90-363 Łodz, Poland
4
Department of Clinical Immunology, Medical University of Warsaw, Nowogrodzka 59, 02-006 Warsaw, Poland
5
Laboratory of Microbial Immunochemistry and Vaccines, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wroclaw, Poland
6
Medical Microbiology Laboratory, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wroclaw, Poland
7
Bacteriophage Laboratory, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114 Wroclaw, Poland
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Molecules 2021, 26(17), 5404; https://doi.org/10.3390/molecules26175404
Submission received: 5 August 2021 / Revised: 22 August 2021 / Accepted: 26 August 2021 / Published: 6 September 2021
(This article belongs to the Special Issue Isolation, Identification and Applications of Polysaccharides)
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Abstract

We previously described the biosynthesis, isolation, and immunosuppressive activity of the selenium-containing polysaccharide fraction isolated from the mycelial culture of Lentinula edodes. Structural studies have shown that the fraction was a protein-containing mixture of high molar mass polysaccharides α- and β-glucans. However, which of the components of the complex fraction is responsible for the immunosuppressive activity non-typical for polysaccharides of fungal origin has not been explained. In the current study, we defined four-polysaccharide components of the Se-containing polysaccharide fraction determined their primary structure and examined the effect on T- and B-cell proliferation. The isolated Se-polysaccharides, α-1,4-glucan (Mw 2.25 × 106 g/mol), unbranched β-1,6-d-glucan, unbranched β-1,3-d-glucan and β-1,3-branched β-1,6-d-glucan (Mw 1.10 × 105 g/mol), are not typical as components of the cell wall of L. edodes. All are biologically active, but the inhibitory effect of the isolated polysaccharides on lymphocyte proliferation was weaker, though more selective than that of the crude fraction.
Keywords: Lentinula edodes; Se-containing polysaccharide; immunosuppressant; T lymphocyte Lentinula edodes; Se-containing polysaccharide; immunosuppressant; T lymphocyte

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MDPI and ACS Style

Klimaszewska, M.; Górska, S.; Łapienis, G.; Kaleta, B.; Górska, S.; Kaszowska, M.; Dawidowski, M.; Gamian, A.; Zagożdżon, R.; Górski, A.; et al. Identification of the Primary Structure of Selenium-Containing Polysaccharides Selectively Inhibiting T-Cell Proliferation. Molecules 2021, 26, 5404. https://doi.org/10.3390/molecules26175404

AMA Style

Klimaszewska M, Górska S, Łapienis G, Kaleta B, Górska S, Kaszowska M, Dawidowski M, Gamian A, Zagożdżon R, Górski A, et al. Identification of the Primary Structure of Selenium-Containing Polysaccharides Selectively Inhibiting T-Cell Proliferation. Molecules. 2021; 26(17):5404. https://doi.org/10.3390/molecules26175404

Chicago/Turabian Style

Klimaszewska, Marzenna, Sabina Górska, Grzegorz Łapienis, Beata Kaleta, Sandra Górska, Marta Kaszowska, Maciej Dawidowski, Andrzej Gamian, Radoslaw Zagożdżon, Andrzej Górski, and et al. 2021. "Identification of the Primary Structure of Selenium-Containing Polysaccharides Selectively Inhibiting T-Cell Proliferation" Molecules 26, no. 17: 5404. https://doi.org/10.3390/molecules26175404

APA Style

Klimaszewska, M., Górska, S., Łapienis, G., Kaleta, B., Górska, S., Kaszowska, M., Dawidowski, M., Gamian, A., Zagożdżon, R., Górski, A., & Turło, J. (2021). Identification of the Primary Structure of Selenium-Containing Polysaccharides Selectively Inhibiting T-Cell Proliferation. Molecules, 26(17), 5404. https://doi.org/10.3390/molecules26175404

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