Next Article in Journal
SR-17018 Stimulates Atypical µ-Opioid Receptor Phosphorylation and Dephosphorylation
Next Article in Special Issue
Mexican Oregano (Lippia graveolens Kunth) as Source of Bioactive Compounds: A Review
Previous Article in Journal
Synthesis of Hierarchical MOR-Type Zeolites with Improved Catalytic Properties
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 26
Issue 15
10.3390/molecules26154510
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Preclinical Bioavailability, Tissue Distribution, and Protein Binding Studies of Erinacine A, a Bioactive Compound from Hericium erinaceus Mycelia Using Validated LC-MS/MS Method

by
Pei-Ching Tsai
1,†,
Yi-Kai Wu
1,†,
Jun-Hao Hu
1,
I-Chen Li
2,
Ting-Wei Lin
2,
Chin-Chu Chen
1,2 and
Chia-Feng Kuo
1,*
1
Department of Food Science, Nutrition, and Nutraceutical Biotechnology, Shih Chien University, Taipei 10462, Taiwan
2
Biotech Research Institute, Grape King Bio Ltd., Taoyuan 32542, Taiwan
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Molecules 2021, 26(15), 4510; https://doi.org/10.3390/molecules26154510
Submission received: 15 June 2021 / Revised: 22 July 2021 / Accepted: 23 July 2021 / Published: 27 July 2021
(This article belongs to the Special Issue Bioactive Compounds from Nature: New Research and Prospects)
Browse Figures
Versions Notes

Abstract

Erinacine A, derived from the mycelia of Hericium erinaceus, has attracted much attention due to its neuroprotective properties. However, very few studies have been conducted on the bioavailability, tissue distribution, and protein binding of erinacine A. This study aimed to investigate the bioavailability, tissue distribution, and protein binding of erinacine A in Sprague-Dawley rats. After oral administration (po) and intravenous administration (iv) of 2.381 g/kg BW of the H. erinaceus mycelia extract (equivalent to 50 mg/kg BW of erinacine A) and 5 mg/kg BW of erinacine A, respectively, the absolute bioavailability of erinacine A was estimated as 24.39%. Erinacine A was detected in brain at 1 h after oral dosing and reached the peak at 8 h. Protein binding assay showed unbound erinacine A fractions in brain to blood ratio is close to unity, supporting passive diffusion as the dominating transport. Feces was the major route for the elimination of erinacine A. This study is the first to show that erinacine A can penetrate the blood-brain barrier of rats by the means of passive diffusion and thus support the development of H. erinaceus mycelia for the improvement of neurohealth.
Keywords: Hericium erinaceus mycelia; erinacine A; bioavailability; tissue distribution; excretion Hericium erinaceus mycelia; erinacine A; bioavailability; tissue distribution; excretion

Share and Cite

MDPI and ACS Style

Tsai, P.-C.; Wu, Y.-K.; Hu, J.-H.; Li, I.-C.; Lin, T.-W.; Chen, C.-C.; Kuo, C.-F. Preclinical Bioavailability, Tissue Distribution, and Protein Binding Studies of Erinacine A, a Bioactive Compound from Hericium erinaceus Mycelia Using Validated LC-MS/MS Method. Molecules 2021, 26, 4510. https://doi.org/10.3390/molecules26154510

AMA Style

Tsai P-C, Wu Y-K, Hu J-H, Li I-C, Lin T-W, Chen C-C, Kuo C-F. Preclinical Bioavailability, Tissue Distribution, and Protein Binding Studies of Erinacine A, a Bioactive Compound from Hericium erinaceus Mycelia Using Validated LC-MS/MS Method. Molecules. 2021; 26(15):4510. https://doi.org/10.3390/molecules26154510

Chicago/Turabian Style

Tsai, Pei-Ching, Yi-Kai Wu, Jun-Hao Hu, I-Chen Li, Ting-Wei Lin, Chin-Chu Chen, and Chia-Feng Kuo. 2021. "Preclinical Bioavailability, Tissue Distribution, and Protein Binding Studies of Erinacine A, a Bioactive Compound from Hericium erinaceus Mycelia Using Validated LC-MS/MS Method" Molecules 26, no. 15: 4510. https://doi.org/10.3390/molecules26154510

APA Style

Tsai, P.-C., Wu, Y.-K., Hu, J.-H., Li, I.-C., Lin, T.-W., Chen, C.-C., & Kuo, C.-F. (2021). Preclinical Bioavailability, Tissue Distribution, and Protein Binding Studies of Erinacine A, a Bioactive Compound from Hericium erinaceus Mycelia Using Validated LC-MS/MS Method. Molecules, 26(15), 4510. https://doi.org/10.3390/molecules26154510

Article Metrics

Back to TopTop