Unlocking the Real Potential of Black Soldier Fly (Hermetia illucens) Larvae Protein Derivatives in Pet Diets
Abstract
:1. Introduction
2. Results
2.1. Glucosamine Content
2.2. Proteinase (Trysin) Inhibitory Assay
2.3. Erythrocyte Membrane Stability Assay
2.4. Reactive Oxygen Species (ROS) Production by Macrophages
2.5. ROS Production by PMA Activated HL-60 Cells
2.6. Metabolic Activity of HL-60 Cells
3. Discussion
3.1. Glucosamine Content
3.2. Proteinase Inhibition
3.3. Erythrocyte Membrane Stability
3.4. ROS Production by Macrophages
3.5. ROS Production by HL-60 Cells
3.6. Cellular Metabolic Activity
4. Materials and Methods
4.1. Reagents
4.2. Raw Materials
4.3. Glucosamine Content
4.4. Proteinase Inhibitory Assay
4.5. Erythrocyte Membrane Stability Assay
4.6. Cellular ROS Production
4.6.1. ROS Production by Macrophages
4.6.2. ROS Production by PMA Activated HL-60 Cells
4.7. Metabolic Acitivty of HL-60 Cells
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Sample | Glucosamine Content (%) |
---|---|
Pasteurized minced meat of BSFL | 0.5 ± 0.2 a |
Hydrolyzed and pasteurized minced meat of BSFL | 0.5 ± 0.2 a |
Hydrolysate of water-soluble BSFL proteins | 0.4 ± 0.0 a |
Chicken meal | 0.4 ± 0.0 a |
Sample | Moisture Content (g/kg) | Crude Protein Content (g/kg) | Crude Fat Content (g/kg) |
---|---|---|---|
Pasteurized minced meat of BSFL | 700.0 | 120.0 | 122.5 |
Hydrolyzed and pasteurized minced meat of BSFL | 700.0 | 120.0 | 122.5 |
Hydrolysate of water-soluble BSFL proteins | 55.0 | 455.0 | 35.0 |
Chicken meal | 60.0 | 700.0 | 120.0 |
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Mouithys-Mickalad, A.; Tome, N.M.; Boogaard, T.; Chakraborty, A.; Serteyn, D.; Aarts, K.; Paul, A. Unlocking the Real Potential of Black Soldier Fly (Hermetia illucens) Larvae Protein Derivatives in Pet Diets. Molecules 2021, 26, 4216. https://doi.org/10.3390/molecules26144216
Mouithys-Mickalad A, Tome NM, Boogaard T, Chakraborty A, Serteyn D, Aarts K, Paul A. Unlocking the Real Potential of Black Soldier Fly (Hermetia illucens) Larvae Protein Derivatives in Pet Diets. Molecules. 2021; 26(14):4216. https://doi.org/10.3390/molecules26144216
Chicago/Turabian StyleMouithys-Mickalad, Ange, Nuria Martin Tome, Thomas Boogaard, Arpita Chakraborty, Didier Serteyn, Kees Aarts, and Aman Paul. 2021. "Unlocking the Real Potential of Black Soldier Fly (Hermetia illucens) Larvae Protein Derivatives in Pet Diets" Molecules 26, no. 14: 4216. https://doi.org/10.3390/molecules26144216