Micro-Raman Characterization of Structural Features of High-k Stack Layer of SOI Nanowire Chip, Designed to Detect Circular RNA Associated with the Development of Glioma
Abstract
:1. Introduction
2. Results
2.1. Monitoring of the Quality of h-k-SOI-NW Chip with High-k Cover Dielectrics of Different Composition by Micro-Raman Spectroscopy
2.2. Demonstration of the Detection Capabilities of Synthetic Analogues of circRNA-oDNA, Associated with Glioma by h-k-SOI-NW Chip
2.3. Demonstration of the Detection Capabilities of circ-SHKBP1, Associated with Glioma in Blood Plasma by h-k-SOI-NW Chip
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Oligonucleotides
4.3. Equipment
4.4. Fabrication of h-k-SOI-NW Chip
4.5. Modification of h-k-SOI-NW Chip Surface
4.6. Covalent Immobilization of Oligonucleotide Probes
4.7. Target oDNAs in Buffer Solutions Preparation
4.8. Plasma Samples
4.9. Electrical Measurements
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Plasma Sample No. | Age | Sex | Diagnosis | Morphological Characteristic |
---|---|---|---|---|
005 | 54 | Female | Neoplasm of an uncertain nature of the brain and central nervous system | Brain tissue with a marginal zone of glioma in one of the fragments, within the studied biopsy |
96 | 70 | Male | Prostatic hyperplasia | - |
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Ivanov, Y.D.; Malsagova, K.A.; Popov, V.P.; Kupriyanov, I.N.; Pleshakova, T.O.; Galiullin, R.A.; Ziborov, V.S.; Dolgoborodov, A.Y.; Petrov, O.F.; Miakonkikh, A.V.; et al. Micro-Raman Characterization of Structural Features of High-k Stack Layer of SOI Nanowire Chip, Designed to Detect Circular RNA Associated with the Development of Glioma. Molecules 2021, 26, 3715. https://doi.org/10.3390/molecules26123715
Ivanov YD, Malsagova KA, Popov VP, Kupriyanov IN, Pleshakova TO, Galiullin RA, Ziborov VS, Dolgoborodov AY, Petrov OF, Miakonkikh AV, et al. Micro-Raman Characterization of Structural Features of High-k Stack Layer of SOI Nanowire Chip, Designed to Detect Circular RNA Associated with the Development of Glioma. Molecules. 2021; 26(12):3715. https://doi.org/10.3390/molecules26123715
Chicago/Turabian StyleIvanov, Yuri D., Kristina A. Malsagova, Vladimir P. Popov, Igor N. Kupriyanov, Tatyana O. Pleshakova, Rafael A. Galiullin, Vadim S. Ziborov, Alexander Yu. Dolgoborodov, Oleg F. Petrov, Andrey V. Miakonkikh, and et al. 2021. "Micro-Raman Characterization of Structural Features of High-k Stack Layer of SOI Nanowire Chip, Designed to Detect Circular RNA Associated with the Development of Glioma" Molecules 26, no. 12: 3715. https://doi.org/10.3390/molecules26123715