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Volume 26
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10.3390/molecules26113137
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Article

Scale-Up Preparation of Crocins I and II from Gardeniajasminoides by a Two-Step Chromatographic Approach and Their Inhibitory Activity Against ATP Citrate Lyase

by
Shuguang Guan
1,2,
Qiaoli Pu
1,
Yinan Liu
3,
Honghong Wu
4,
Wenbo Yu
1,
Zifeng Pi
2,5,*,
Shu Liu
2,
Fengrui Song
2,
Jingya Li
3 and
De-An Guo
1,3,*
1
College of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, China
2
Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
3
Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
4
University of Chinese Academy of Sciences, Beijing 100049, China
5
Changchun Sunnytech Co., Ltd., Changchun 130061, China
*
Authors to whom correspondence should be addressed.
Molecules 2021, 26(11), 3137; https://doi.org/10.3390/molecules26113137
Submission received: 3 May 2021 / Revised: 17 May 2021 / Accepted: 20 May 2021 / Published: 24 May 2021
(This article belongs to the Special Issue Analytical and Preparative Hyphenation of Countercurrent Chromatography (CCC/CPC) to Structure Elucidation Methods in the Field of Natural Product Recovery and Identification)
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Abstract

Crocins are highly valuable natural compounds for treating human disorders, and they are also high-end spices and colorants in the food industry. Due to the limitation of obtaining this type of highly polar compound, the commercial prices of crocins I and II are expensive. In this study, macroporous resin column chromatography combined with high-speed counter-current chromatography (HSCCC) was used to purify crocins I and II from natural sources. With only two chromatographic steps, both compounds were simultaneously isolated from the dry fruit of Gardenia jasminoides, which is a cheap herbal medicine distributed in a number of countries. In an effort to shorten the isolation time and reduce solvent usage, forward and reverse rotations were successively utilized in the HSCCC isolation procedure. Crocins I and II were simultaneously obtained from a herbal resource with high recoveries of 0.5% and 0.1%, respectively, and high purities of 98.7% and 99.1%, respectively, by HPLC analysis. The optimized preparation method was proven to be highly efficient, convenient, and cost-effective. Crocins I and II exhibited inhibitory activity against ATP citrate lyase, and their IC50 values were determined to be 36.3 ± 6.24 and 29.7 ± 7.41 μM, respectively.
Keywords: crocins; preparation method; macroporous resin column chromatography; high-speed counter-current chromatography; inhibitory activity on ATP citrate lyase crocins; preparation method; macroporous resin column chromatography; high-speed counter-current chromatography; inhibitory activity on ATP citrate lyase

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MDPI and ACS Style

Guan, S.; Pu, Q.; Liu, Y.; Wu, H.; Yu, W.; Pi, Z.; Liu, S.; Song, F.; Li, J.; Guo, D.-A. Scale-Up Preparation of Crocins I and II from Gardeniajasminoides by a Two-Step Chromatographic Approach and Their Inhibitory Activity Against ATP Citrate Lyase. Molecules 2021, 26, 3137. https://doi.org/10.3390/molecules26113137

AMA Style

Guan S, Pu Q, Liu Y, Wu H, Yu W, Pi Z, Liu S, Song F, Li J, Guo D-A. Scale-Up Preparation of Crocins I and II from Gardeniajasminoides by a Two-Step Chromatographic Approach and Their Inhibitory Activity Against ATP Citrate Lyase. Molecules. 2021; 26(11):3137. https://doi.org/10.3390/molecules26113137

Chicago/Turabian Style

Guan, Shuguang, Qiaoli Pu, Yinan Liu, Honghong Wu, Wenbo Yu, Zifeng Pi, Shu Liu, Fengrui Song, Jingya Li, and De-An Guo. 2021. "Scale-Up Preparation of Crocins I and II from Gardeniajasminoides by a Two-Step Chromatographic Approach and Their Inhibitory Activity Against ATP Citrate Lyase" Molecules 26, no. 11: 3137. https://doi.org/10.3390/molecules26113137

APA Style

Guan, S., Pu, Q., Liu, Y., Wu, H., Yu, W., Pi, Z., Liu, S., Song, F., Li, J., & Guo, D.-A. (2021). Scale-Up Preparation of Crocins I and II from Gardeniajasminoides by a Two-Step Chromatographic Approach and Their Inhibitory Activity Against ATP Citrate Lyase. Molecules, 26(11), 3137. https://doi.org/10.3390/molecules26113137

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