Isolation, Physicochemical Properties, and Structural Characteristics of Arabinoxylan from Hull-Less Barley

Yao, Haoyingye; Wang, Yuxiao; Yin, Junyi; Nie, Shaoping; Xie, Mingyong

doi:10.3390/molecules26103026

Open AccessArticle

Isolation, Physicochemical Properties, and Structural Characteristics of Arabinoxylan from Hull-Less Barley

by

Haoyingye Yao

¹,

Yuxiao Wang

¹,

Junyi Yin

¹,

Shaoping Nie

¹

and

Mingyong Xie

^1,2,*

¹

State Key Laboratory of Food Science and Technology, China-Canada Joint Lab of Food Science and Technology (Nanchang), Nanchang University, 235 Nanjing East Road, Nanchang 330047, China

²

National R&D Center for Freshwater Fish Processing, Jiangxi Normal University, Nanchang 330022, China

^*

Author to whom correspondence should be addressed.

Molecules 2021, 26(10), 3026; https://doi.org/10.3390/molecules26103026

Submission received: 17 April 2021 / Revised: 10 May 2021 / Accepted: 14 May 2021 / Published: 19 May 2021

(This article belongs to the Special Issue Isolation, Identification and Applications of Polysaccharides)

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Abstract

Arabinoxylan (HBAX-60) was fractioned from alkaline-extracted arabinoxylan (HBAX) in the whole grain of hull-less barley (Hordeum vulgare L. var. nudum Hook. f. Poaceae) by 60% ethanol precipitation, which was studied for physicochemical properties and structure elucidation. Highly purified HBAX-60 mainly composed of arabinose (40.7%) and xylose (59.3%) was created. The methylation and NMR analysis of HBAX-60 indicated that a low-branched β-(1→4)-linked xylan backbone possessed un-substituted (1,4-linked β-Xylp, 36.2%), mono-substituted (β-1,3,4-linked Xylp, 5.9%), and di-substituted (1,2,3,4-linked β-Xylp, 12.1%) xylose units as the main chains, though other residues (α-Araf-(1→, β-Xylp-(1→, α-Araf-(1→3)-α-Araf-(1→ or β-Xylp-(1→3)-α-Araf-(1→) were also determined. Additionally, HBAX-60 exhibited random coil conformation in a 0.1 M NaNO₃ solution. This work provides the properties and structural basis of the hull-less barley-derived arabinoxylan, which facilitates further research for exploring the structure–function relationship and application of arabinoxylan from hull-less barley.

Keywords: hull-less barley; arabinoxylan; structural characteristics

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MDPI and ACS Style

Yao, H.; Wang, Y.; Yin, J.; Nie, S.; Xie, M. Isolation, Physicochemical Properties, and Structural Characteristics of Arabinoxylan from Hull-Less Barley. Molecules 2021, 26, 3026. https://doi.org/10.3390/molecules26103026

AMA Style

Yao H, Wang Y, Yin J, Nie S, Xie M. Isolation, Physicochemical Properties, and Structural Characteristics of Arabinoxylan from Hull-Less Barley. Molecules. 2021; 26(10):3026. https://doi.org/10.3390/molecules26103026

Chicago/Turabian Style

Yao, Haoyingye, Yuxiao Wang, Junyi Yin, Shaoping Nie, and Mingyong Xie. 2021. "Isolation, Physicochemical Properties, and Structural Characteristics of Arabinoxylan from Hull-Less Barley" Molecules 26, no. 10: 3026. https://doi.org/10.3390/molecules26103026

APA Style

Yao, H., Wang, Y., Yin, J., Nie, S., & Xie, M. (2021). Isolation, Physicochemical Properties, and Structural Characteristics of Arabinoxylan from Hull-Less Barley. Molecules, 26(10), 3026. https://doi.org/10.3390/molecules26103026

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Isolation, Physicochemical Properties, and Structural Characteristics of Arabinoxylan from Hull-Less Barley

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