Next Article in Journal
G-Protein Coupled Receptors (GPCRs) in Insects—A Potential Target for New Insecticide Development
Previous Article in Journal
Calculation Methods of Solution Chemical Potential and Application in Emulsion Microencapsulation
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Molecules
Volume 26
Issue 10
10.3390/molecules26102992
molecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Synthesis, Anticancer Screening of Some Novel Trimethoxy Quinazolines and VEGFR2, EGFR Tyrosine Kinase Inhibitors Assay; Molecular Docking Studies

by
Abdulmalik S. Altamimi
1,*,
Adel S. El-Azab
2,
Sami G. Abdelhamid
3,
Mubarak A. Alamri
1,
Ashraf H. Bayoumi
4,
Safar M. Alqahtani
1,
Alhumaidi B. Alabbas
1,
Ali I. Altharawi
1,
Manal A. Alossaimi
1 and
Menshawy A. Mohamed
1,4,*
1
Department of Pharmaceutical Chemistry, College of Pharmacy, Prince Sattam bin Abdulaziz University, Alkharj 11942, Saudi Arabia
2
Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia
3
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Al-Azhar University, Cairo 11884, Egypt
4
Department of Pharmaceutical Organic Chemistry, Faculty of Pharmacy, Al-Azhar University, Cairo 11884, Egypt
*
Authors to whom correspondence should be addressed.
Molecules 2021, 26(10), 2992; https://doi.org/10.3390/molecules26102992
Submission received: 12 April 2021 / Revised: 5 May 2021 / Accepted: 11 May 2021 / Published: 18 May 2021
(This article belongs to the Section Medicinal Chemistry)
Browse Figures
Versions Notes

Abstract

:
A new series of 8-methoxy-2-trimethoxyphenyl-3-substituted quinazoline-4(3)-one compounds were designed, synthesized, and screened for antitumor activity against three cell lines, namely, Hela, A549, and MDA compared to docetaxel as reference drug. The molecular docking was performed using Autodock Vina program and 20 ns molecular dynamics (MD) simulation was performed using GROMACS 2018.1 software. Compound 6 was the most potent antitumor of the new synthesized compounds and was evaluated as a VEGFR2 and EGFR inhibitor with (IC50, 98.1 and 106 nM respectively) compared to docetaxel (IC50, 89.3 and 56.1 nM respectively). Compounds 2, 6, 10, and 8 showed strong cytotoxic activities against the Hela cell line with IC50 of, 2.13, 2.8, 3.98, and 4.94 µM, respectively, relative to docetaxel (IC50, 9.65 µM). Compound 11 showed strong cytotoxic activity against A549 cell line (IC50, 4.03 µM) relative to docetaxel (IC50, 10.8 µM). Whereas compounds 6 and 9 showed strong cytotoxic activity against MDA cell line (IC50, 0.79, 3.42 µM, respectively) as compared to docetaxel (IC50, 3.98 µM).

Graphical Abstract

1. Introduction

Cancer is one of the world’s most prominent health-related diseases, which makes the search for safe and efficient anticancer compounds a major goal for researchers. Many heterocyclic compounds have been intensively studied to classify new anticancer agents. Quinazoline is one of the most common groups of heterocyclic compounds used in medicine because of its broad variety of biological activities. We are particularly interested in the present work with quinazoline derivatives that have been identified as a class of heterocyclic cancer chemotherapeutic agents with significant therapeutic effects [1,2]. For example, the effects of 2-chloro-4-anilino quinazoline derivatives as dual inhibitors for VEPGR and EGFR [3]. Some methoxy quinazolines are active as anticancer compounds [4]. Quinazoline-isatin conjugates as an epidermal growth factor inhibitor [5]. 2-substituted mercapto-3-(3,4,5-trimethoxybenzyl)-4(3H)-quinazolinone analogues show antitumor activity [6]. Trimethoxyanilide based on 4 (3H)-quinazolinone scaffolds as an antitumor compound [7]. Some quinazolinone nuclei are frequently used in medicinal chemistry [8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24]. Some substituted 2-mercapto-4(3H)-quinazolinones and 2,3-disubstitued 4(3H)-quinazolinone derivatives work as anti-inflammatory and analgesic agents, such as COX-1/2 inhibitory activities [9,10]. Two identical anilinoquinazoline rings with ethylaminoethyl linker and N-ethylaminomorpholine moiety to enhance water solubility were synthesized and evaluated as inhibitors of epidermal growth factor receptor (EGFR) tyrosine kinase with IC (50) values in the low micromolar range [25]. Some dimers and substituted amino derivatives of quinazoline were synthesized, docked into ATP binding sites of epidermal growth factor receptor (EGFR) tyrosine kinase, and evaluated as antitumor compounds against MCF7 cells with IC50 = 0.06 μg/mL; 1.64 nmol/mL [26]. Benzofuran and indole derivatives of 4-aminoquinazoline are inhibitors of epidermal growth factor receptor (EGFR) tyrosine kinase phosphorylation. A heterocyclic scaffold has been found to lead to heterocyclic quinazolines with good biological activity against a panel of EGFR-positive cell lines, such as the human lung cancer (A549), epithelial colorectal adenocarcinoma (Caco-2), hepatocellular carcinoma (C3A), breast adenocarcinoma (MCF-7), and cervical cancer (Hela) cell lines with reduced side effects [27].
Quinazoline has become an important pharmacophore for anticancer research since the recent FDA approval of some quinazoline derivatives as anticancer drugs, such as Raltitrexed (TomudexTM) and nolatrexed (thymitaqTM) as shown in Figure 1, as well as gefitinib (IressaTM), lapatinib (TykerbTM also known as GW-572016), Vandetanib (ZactimaTM), afatinib (GilotrifTM), and erlotinib (TarcevaTM).
In our present study, we designed a number of quinazoline derivatives bearing trimethoxy phenyl moiety in position 2 and insertion of methoxy at position 8 to give different electronic, steric, and lipophilic characters that might help improve the antitumor activity of the newly designed scaffolds. In addition, we kept the substitution at position 3 in an attempt to get optimized quinazoline derivatives with promising anticancer activity (Figure 2).

2. Results and Discussion

2.1. Chemistry

The synthesis of the target compounds is depicted in scheme 1. The starting material 8-methoy-2-trimethoxyphenyl-4H-benzo[d](1,3)oxazin-4-one (3) was prepared according to a reported procedure [28] by allowing N-(trimethoxybenzoyl)-3-methoxyanthranilic acid to boil with acetic anhydride, then compound 3 allowed was fused with hydrazine to get 3-amino-8-methoxy-2-trimethoxyphenylquanazoline-4(3H)-one (4). IR spectra of compound 4 showed absorption bands at 3221.35, 3387.83, and 1655.19 cm−1 due to stretching vibration of the NH2 group and CO, respectively, and 1H NMR led to the appearance of a singlet of two protons for the NH2 group at 5.842. Compound 3 reacted with formamide to give 8-methoxy-2-trimethoxyphenylquanazoline-4(3H)-one (5), the latter compound showed a stretching band for CO at 1655.67 cm−1, and 1H NMR showed a singlet peak at 8.292 ppm for the NH group. Compound 3 reacted with ammonia to create benzamide derivative 6, IR of compound 6 showed stretching vibration bands for NH2 and NH at 3193.13, 3367.91, and 3389.62 and stretching bands at 1672.66 and 1663.23 for two CO groups. Moreover the 1H NMR data showed a singlet peak for the NH2 group at 6.069 ppm and a singlet peak at 9.782 ppm for NH moiety. Compound 3 reacted with hydrazine hydrate in the presence of ethanol to get hydrazide derivative 7. Compound 7 showed characteristic CO bands of diamide at 1670.63 and 1655.19 and NH, NH2 absorption at 3376.45, 3382.21, 3175.43, and 3295.34 cm−1, in addition to an 1H NMR singlet peak at 4.479 ppm for NH2 and two singlets for NH of diamide at 10.134 and 10.167 ppm. Compound 7 reacted with benzene sulphonyl chloride, which afforded compound 8 with absorption bands of NH groups at 3366.45, 3372.52, and 3385.43 cm−1 and CO band at 1670.65 and 1655.37 cm−1 on the IR spectrum, as well as singlets at 8.5497 and 12.615 ppm for NH protons in 1H NMR. Additionally, compound 4 reacted with benzene sulphonyl chloride to give compound 9 with absorption bands at 1655.82 and 3385.56 cm−1 due to Co and NH, respectively, beside the 1H NMR singlet for NH at 11.885 ppm. Moreover, compound 3 refluxed with methyl amine to give 3-methyl-8-methoxy-2-trimethoxyphenylquanazoline-4(3H)-one (10), the latter compound was confirmed by the presence of the CH3 group at 17.08 and 2.637 ppm in 13C NMR and 1H NMR, respectively. Compound 3 reacted with 3, 4, 5-trimethoxy aniline to create 3-(3, 4, 5-trimethoxyphenyl)-8-methoxy-2-trimethoxyphenylquanazoline-4(3H)-one (11), which was confirmed by the presence of singlet peaks at 56.18, 59.56, and 60.23 ppm and 3.624, 3.768, and 3.964 ppm due to one methoxy group and two trimethoxy groups in 13C NMR and 1H NMR spectrum, respectively (Scheme 1).

2.2. VEGFR2 and EGFR Inhibitory Activity

The synthesized compounds were evaluated for anticancer activity by adapting a reported procedure [29,30,31,32,33] using two enzymes, VEGFR2 and EGFR, as well as three cell lines. The inhibitory activity for VEGFR2 and EGFR was assessed for the most active one (compound 6) and the results are reported as IC50 values. Compound No. 6 proved to have almost 91% inhibitory activity for enzyme VEGFR2 compared to the inhibitory activity of the reference drug, with IC50 values 98.1 to compound 6 and 89.3 for docetaxel for the positive control. Meanwhile, the inhibitory activity for enzyme EGFR was almost 53%, with IC50 value 106 compared to IC 50 value 56.1 for docetaxel as reference drug (Table 1).
The synthesized compounds were also subjected to anticancer assay using three cell line panels. The first cell line was Hela cells using docetaxel as positive control and the results were recorded as IC50 values. Compounds 2, 6, 10, and 8 proved to be the most active Hela cells inhibitors, with IC50 values indicating that they are more potent than docetaxel, with scores of IC50, 2.13, 2.8, 3.98, 4.94 µM, respectively, and 3-methoxy-N-(trimethoxyphenyl) anthranilic acid was the most potent of them as a Hela inhibitor, with almost fivefold inhibitory activity. Compounds 9, 11, and 5 proved to have good inhibitory activity, with IC50 values of 11.70, 12.00, and 12.90 µM, while compounds 4 and 3 had moderate activity as Hela cell inhibitors, with IC50 values 29.80 and 66.50 as compared with that of docetaxel IC50, 9.65 µM (Table 2 and Figure 3). The second one was A549, using docetaxel as positive control, and the results were determined as IC50 values. 8-methoxy-2-trimethoxymethyl-3-trimethoxyphenyl quanazoline-4(3H)-one (11) proved to be the most potent one of them, with almost two-and-a-half-fold more than the reference drug, with an IC50 value of 4.03 µM. Compounds 10, 8, 4, and 9 showed good A549 inhibitory activity, with IC50 values 12.30, 13.00, 18.00, and 19.90 µM, respectively. Compounds 5, 2, 3, and 7 showed moderate A549 inhibitory activity, with IC50 values of 26.30, 29.70, 43.60, and 46.30 µM, respectively. In addition, compound 6 proved to be the least active one, with IC50, 81.00 µM in comparison to docetaxel (IC50, 10.8 µM) (Table 2 and Figure 3).
The third cell line was MDA cells using a positive control and the results are reported as IC50 values. Compounds 6 and 9 proved to be the ones that were more potent than the positive control, with IC50 values of 0.79 and 3.42 µM, respectively, and compound 6 (3-methoxy-2-(trimethoxybenzamido) benzamide) was the most active of all as MDA inhibitor, with a fourfold higher inhibitory activity compared to the reference drug. Respectively, compounds 4 and 5 had good inhibitory activity with MDA cells with IC50, 4.12 and 4.39 µM. Compounds 11, 7, 2, and 8 showed moderate inhibitory activity, with IC50 values 7.06, 9.00, 9.24, and 11.70 µM, respectively. In addition, compound 3 showed the least activity, with an IC50 value of 17.40 µM as compared with docetaxel IC50, 3.98 µM. (Table 2 and Figure 3).

2.3. Molecular Docking Analysis

In order to get an insight into the mechanism of interaction between M6 and EGFR and VEGFR2 tyrosine kinases, a molecular docking analysis was performed using docetaxel and co-crystalized ligands as references. The docking of M6 into the ATP pocket of EGFRL858R/T790M indicates that M6 adapted a binding mode similar to docetaxel as well as the co-crystal ligand; compound 1 had a docking energy score of −7.4 kcal/mol (Figure 4A). Compound 1 is a potent non-covalent inhibitor of EGFR del19 T790M C797S, with an IC50 = 790 nM [34].
M6 was able to form a hydrogen bond between the oxygen atom of methoxy-group and T854 within the hinge region and hydrophobic interaction with side chains of F723, L718, and L844 (Figure 4B). Notably, a mutation of L718 within the P-loop has been reported as a mutation conferring resistance to osimertinib [35]. It was also in close proximity to catalytic lysine residue, K745. In contrast, the docetaxel was involved in two hydrogen bonds, with the nitrogen of N842 and oxygen of D800, as well as several hydrophobic interactions with L844, G796, and L718 (Figure 4C). To confirm the docking results, M6–EGFR complex was subjected to a 20 ns MD simulation to further analyze the molecular mechanism of the ligand–protein interaction. The backbone RMSD of the M6–EGFR complex was calculated to predict the M6–EGFR complex stability over time. As shown in Figure 4A, the complex reached equilibration after ~1.5 ns with a normal fluctuation pattern for the rest of the time (~18.5 ns) between 0.2 and 0.3 Å, indicating a stable dynamic behavior (Figure 5A). The hydrogen bonds are major contributors to the stability of the binding between the protein and the ligand. M6 was found to form up to three hydrogen bonds with the active site of EGFR over the simulation time (Figure 5B).
Similarly, the docking of M6 against the wild type VEGFR2 was performed using sorafenib as a reference. Sorafenib is a nonselective potent ATP-competitive inhibitor of VEGFR2 in which IC50 = 2.3 nM [36]. The results indicate that M6 binds to the ATP binding pocket with a docking energy score of −7.3 kcal/mol. This compound binds to the conserved regions of the ATP site, similarly to docetaxel as well as the co-crystalized ligand, sorafenib (Figure 6A). However, unlike M6, docetaxel was able to bind the regulatory domain pocket (RDP), as was the sorafenib, as both molecules have larger substituents [37].
In term of interactions, M6 was involved in two hydrogen bonds between the nitrogen and oxygen atoms within the benzamide moiety and E885 of the αC helix; as well as with the D1046 residue in the DFG region of the VEGFR2, respectively; and in hydrophobic bonds with the side chain of L1019 (Figure 6B). On the other hand, docetaxel made four hydrogen bonds with V916, L1025, D1046, AND L1049, as well as hydrophobic interactions with K868, L882, G885 L889, I892, and H1026 (Figure 6C). The stability of the M6-VEGFR2 complex was confirmed by MD simulation. The backbone RMSD of the complex showed a normal dynamic behavior with a fluctuation range between 0.1 and 0.2 Å over the 20 ns simulation time. Additionally, this complex formed up to three stable hydrogen bonds over simulation period (Figure 7).
The MD summation results suggest a stable binding of M6 within the ATP binding pocket of EGFR and VEGFR2 due to the stable hydrogen bonds formed with the proteins. These results are in line with the docking and in vitro results supporting the notion that this compound might act as a dual EGFR and VEGFR2 tyrosine kinase inhibitor.

3. Materials and Methods

3.1. Chemistry

All melting points were determined in open capillaries and are uncorrected. Microanalyses were conducted on a Perkin–Elmer 2408 analyzer (Perkin Elmer, USA). Thin layer chromatography was performed on Merck 5 × 10 cm plates. Samples were percolated with silica gel GF254 using (EtOAc, hexane 1:10) as the solvent system and a short wavelength UV light for visualization. All fine chemicals and reagents used were purchased from (Aldrich chemical Co. U.S.A.) 1 HNMR samples were recorded on a Bruker 500 MHz spectrophotometer (Bruker AXS Inc., Switzerland). Chemical shifts are in δ (ppm) values downfield from tetramethyl siloxane as an internal standard. The mass spectra were measured on Waters Micromass (Water AQUITY UPLC System LCT Premier XE Serial no: KE468, USA).
3-methoxy-N-(trimethoxyphenyl) anthranilic acid (2)
A mixture of 3-methyl anthranilic acid (1, 2.37 g, 0.01 mol) and 3,4,5-trimethoxybenzoyl chloride (0.01 mol) in dry pyridine (50 mL) was heated under reflux for 12 h, then cooled and poured onto dilute HCL. The separated solid was filtered, washed with water, dried, and recrystallized from MeOH; 1H NMR (500 MHz, DMSO-d6); δ 3.732 (s, 3H, phenyl-OCH3), 3.85 (s, 9H, phenyl-OCH3), 7.22 (d, 1H, J = 6.0 Hz, phenyl-H), 7.43 (d, 2H, J = 5.5 Hz, phenyl-H), 7.506 (d, H, J = 8.5 Hz, quinazoline-H), 7.80–7.81 (d, 1H, J = 5.5 Hz, quinazoline-H), 7.82–7.84 (d, 1H, J = 6.5 Hz, quinazoline-H), 7.96 (s, 2H, phenyl-H), 9.79 (s, 1H, NHCO), 10.17 (s, 1H, -COOH); 13C NMR (125 MHz, DMSO-d6) d; 56.08, 60.12, 105.21, 114.86, 121.25, 125.73, 126.84, 129.48, 130.35, 139.89, 152.41, 154.74, 164.60, 167.25. MS (m/z): [M+1]: 362.1215.
8-methoxy-2-trimethoxyphenyl-4H-benzo[d](1,3)oxazin-4-one (3)
Cyclization of N-(trimethoxybenzoyl)-3-methoxyanthranilic acid (2, 2.37 g, 0.01 mol) was performed by boiling in (50 mL) acetic anhydride, after which it was cooled and poured onto water. The separated solid was filtered, dried, and recrystallized from MeOH; yield, 83%; mp: 182–184 °C; IR (KBr) ν max/cm−1 1655.11 (CO); 1H NMR (500 MHz, DMSO-d6); δ 3.631 (s, 3H, phenyl-OCH3), 3.73 (s, 9H, phenyl-OCH3), 7.21–7.25 (m, 3H, quinazoline-H),) 7.26 (s, 2H, phenyl-H), 9.81 (s, 1H, -CONH); 13C NMR (125 MHz, DMSO-d6) δ 56.08, 60.12, 105.21, 114.86, 121.25, 125.73, 126.84, 129.48, 130.35, 139.89, 152.41, 154.74, 164.60, 167.25. MS (m/z): [M+1]: 344.1112.
3-amino-8-methoxy-2-trimethoxyphenylquanazoline-4(3H)-one (4)
A mixture of 4H-1,3-benzoxazin-4-one derivative (3, 3.43 g, 0.01 mol) and hydrazine (15 mL) was fused for 6 h, poured in water, filtered, dried, and recrystallized from MeOH; yield, 81%; mp: 182–184 °C; IR (KBr) νmax/cm−1 3221.35, 3387.83 (NH2); 1655.19 (CO); 1H NMR (500 MHz, DMSO-d6), 4: δ 5.842 (s, 2H, -NH2), 3.617 (s, 3H, phenyl-OCH3), 3.683 (s, 9H, phenyl-OCH3), 5.841 (s, 2H, -NH2), 7.013 (s, 2H, phenyl-H), 7.300–7.316 (d, 1H, J = 8.0 Hz, quinolone-H), 7.399–7.431 (m, 1H, quinazoline-H), 7.585–7.601 (d, 1H, J = 8.0 Hz, quinolone-H). 13C NMR (125 MHz, DMSO-d6) δ 55.62.56.06, 56.12, 60.12, 105.13, 115.21, 116.85, 121.78, 126.76, 127.36, 139.17, 140.00, 150.34, 152.79, 164.55, 162.26. MS (m/z): [M+1]: 358.1378.
8-methoxy-2-trimethoxyphenylquanazoline-4(3H)-one (5)
A mixture of 4H-1,3-Benzoxazin-4-one derivative (3, 3.43 g, 0.01 mol) and formamide (30 mL) was heated under reflux for 16 h. On cooling, the separated solid was filtered, washed with water, and recrystallized from EtOH; yield, 78%; mp: 242–244 °C; IR (KBr) νmax/cm−1 1655.67 (CO); 1H NMR (500 MHz, DMSO-d6), 5: δ 2.488 (s, 3H, phenyl-CH3), 7.330 (d, 1H, J = 7.5 Hz, phenyl-H), 7.489 (d, 2H, J = 5.5 Hz, phenyl-H) 7.583 (d, 1H, J = 7.5 Hz, quinazoline-H), 7.758 (d, 1H, J = 7.5 Hz, quinazoline-H), 7.865 (d, 1H, J = 7.5 Hz, quinazoline-H), 8.083 (s, 1H, phenyl-H) 8.132 (s, H, phenyl-H) 8.292 (s, 1H, quinazoline-NH), 13C NMR (125 MHz, DMSO-d6) δ 16.53, 125.23, 125.43, 125.57, 128.03, 128.46, 128.68, 129.25, 130.83, 132.17, 133.65, 138.23, 152.28, 164.43, 170.12. MS (m/z): [M+1]: 343.1274.
3-methoxy-2-(trimethoxybenzamido) benzamide (6)
A mixture of 4H-1,3-benzoxazin-4-one derivative (3, 3.43 g, 0.01 mol) and ammonia solution (20 mL) was heated under reflux for 12 h. It was then poured onto water, filtered, dried, and recrystallized from dioxane; yield, 92%; mp: 132–134 °C; IR (KBr) νmax/cm−1 3193.13, 3367.91(NH2), 3389.62 (NH), 1672.66, 1663.23 (CO); 1H NMR (500 MHz, DMSO-d6), 6: δ 3.760 (s, 3H, phenyl-OCH3), 3.860 (s, 9H, phenyl-OCH3), 6.069(s, 2H, -NH2), 6.478–6.509 (m, 1H, phenyl-H), 6.846 (d, 1H, J = 15.0 Hz, phenyl-H), 7.094 (d, 1H, J = 8.0 Hz, phenyl-H), 7.027–7.284 (m, 2H, phenyl-H) 9.782 (s, 1H, CO-NH). 13C NMR (125 MHz, DMSO-d6) δ 55.44, 55.85, 55.90, 59.99, 119.25, 122.66, 128.36, 138.47, 139.24, 139.59, 146.79, 147.40, 152.54, 165.40, 165.86, 168.41. MS (m/z): [M-1]: 361.1216.
3-methoxy-2-(trimethoxybenzamido)-benzoylhydrazide (7)
A mixture of 4H-1,3-benzoxazin-4-one derivative (3, 3.43 g, 0.01 mol) and hydrazine hydrate (85% 15 mL) in EtOH (30 mL) was heated under reflux for 2 h. The obtained solid was filtered, washed with water, dried, and recrystallized from EtOH; yield, 83%; mp: 195–197 °C; IR (KBr) νmax/cm−1 3175.43, 3295.34,{NH2), 3376.45, 3382.21, (NH); 1670.63, 1655.19 (CO); 1H NMR (500 MHz, DMSO-d6), 7: δ 3.747 (s, 3H, phenyl-OCH3), 3.881 (s, 9H, phenyl-OCH3), 4.479 (s, 2H, -NH2), 7.051 (d, 1H, J 7.5 Hz, phenyl-H), 7.118 (d, 1H, J = 8.0 Hz, phenyl-H), 7.285–7.300 (d, 1H, J = 7.5 Hz, phenyl-H) 7.316–7.384 (m, 2H, phenyl-H) 10.134 (s, 1H, -CONHNH2), 10.167 (s, 1H, -NHCO). 13C NMR (125 MHz, DMSOd6) δ 56.03, 56.24, 60.11, 59.99, 105.33, 113.37, 120.42, 123.51, 127.89, 129.10, 134.73, 139.87, 152.45, 155.49, 164.79, 165.52. MS (m/z): [M−1]: 374.1349.
3-methoxy-2-(trimethoxybenzamido)-N-[benzenesulphonyl]benzoylhydrazide (8)
A mixture of 3-methoxy-2-(trimethoxybenzamido)-benzoylhydrazide (7, 0.01 mol, 3.75 g) and benzene sulphonyl chloride (0.01 mol, 1.76 g) in 15 mL dioxane containing triethylamine (0.02 mol, 2.02 g) was refluxed for 5 h. The reaction mixture was cooled down and the solvent was removed under reduced pressure; the recovered solid was filtered, washed with water, dried, and recrystallized from ethanol; yield, 81%; mp: 263–265 °C; IR (KBr) νmax/cm−1 3366.45, 3372.52, 3385.43 (NH), 1670.65, 1655.37 (CO); 1H NMR (500 MHz, DMSO-d6), 8: δ 3.629.(s, 3H, phenyl-OCH3), 3.942 (s, 9H, phenyl-OCH3), 7.281 (d, 1H, J = 5.0 Hz, phenyl-H),.7.319 (d, 2H, J = 8.0 Hz, phenyl-H), 7.359–7.374 (m, 3H, quinazoline-H), 7.390–7.441 (m, 2H, phenyl-H), 7.566 (2, 2H, J = 7.5 Hz, phenyl-H), 8.5497 (s, 1H, -CONHNH), 12.615 (s, 2H, -CONH). 13C NMR (125 MHz, DMSO-d6) δ 56.19.56.28, 60.18, 105.20, 119.38, 116.85, 121.78, 126.76, 127.36, 127.80, 139.12, 140.08, 150.42, 152.67, 164.36, 167.34. MS (m/z): [M−1]: 514.1275.
3-benzenesulfamoyl-8-methoxy-2-trimethoxyphenylquanazoline-4(3H)-one (9)
A mixture of 3-amino-8-methoxy-2-trimethoxyphenylquanazoline-4(3H)-one (4, 0.01 mol, 3.57 g) and benzene sulphonyl chloride (0.01 mol, 1.76 g) in 15 mL dioxane containing triethylamine (0.02 mol, 2.02 g) was refluxed for 6 h. The reaction mixture was cooled down and the solvent was removed under reduced pressure. The recovered solid was filtered, washed with water, dried, and recrystallized from ethanol; yield, 80%; mp: 244–246 °C; IR (KBr) νmax/cm−1 3385.56 (NH), 1655.82 (CO); 1H NMR (500 MHz, DMSO-d6) 9: δ 187 3.632 (s, 3H, phenyl-OCH3), 3.748 (s, 9H, phenyl-OCH3), 6.7288–7.860 (m, 2H, phenyl-H), 7.224–7.290 (m, 3H, phenyl-H), 7.368–7.459 (m, 3H, phenyl-H), 7.480–7.537 (m, 3H, phenyl-H) 11.885 (s, 1H, NHSO2). 13C NMR (125 MHz, DMSO-d6) δ 56.15, 59.93, 107.04, 116.06, 117.31, 121.60, 126.11, 128.34, 132.61, 135.99, 138.28, 139.85, 151.63, 154.16, 154.43, 160.06. MS (m/z): [M−1]: 496.1171.
3-methyl-8-methoxy-2-trimethoxyphenylquanazoline-4(3H)-one (10)
A mixture of 4H-1,3-benzoxazin-4-one derivative (3, 3.43 g, 0.01 mol) and methyl amine (0.096 g, 0.003 mol) in dry pyridine (15 mL) was heated under reflux for 18 h. The solvent was evaporated under reduced pressure. The obtained solid was filtered, washed with diluted HCl, and crystallized from glacial acetic acid; yield, 85%; mp: 218–220 °C; IR (KBr) νmax/cm−1 1655.73 (CO); 1H NMR (500 MHz, DMSO-d6), 10: δ 2.637 (s, 3H,-CH3), 3.913 (s, 3H, phenyl-OCH3), 3.929 (s, 9H, phenyl-OCH3), 7.358 (d, 1H, J = 8.5 Hz, phenyl-H), 7.357–7.388 (m, 1H, quinazoline-H), 7.518 (s, 2H, phenyl-H), 7.641–7.656 (d, 1H, J = 7.5 Hz, quinazoline-H), 8.045 (d, 1H, J = 7.5 Hz, quinazoline-H). 13C NMR (125 MHz, DMSO-d6) δ 17.08, 56.31, 61.04, 59.99, 105.31, 116.59, 125.56, 126.19, 127.56, 135.97, 137.39, 141.87, 145.22, 153.23, 155.48, 160.16. MS (m/z): [M+1]: 357.1083.
8-methoxy-2-trimethoxymethyl-3-trimethoxyphenyl quanazoline-4(3H)-one (11)
A mixture of 4H-1,3-benzoxazin-4-one derivative (3, 3.43 g, 0.01mol) and 3, 4, 5-trimethoxy aniline (1.89 g, 0.01 mol) in dry pyridine (20 mL) was heated under reflux for 16 h. The solvent was evaporated under reduced pressure. The obtained solid was filtered, washed with diluted HCl, and crystallized from glacial acetic acid; yield, 83%; mp: 237–239 °C; IR (KBr) νmax/cm−1 1655.84 (CO); 1H NMR (500 MHz, DMSO-d6), 11: δ 3.624 (s, 3H, phenyl-OCH3), 3.768 (s, 9H, phenyl-OCH3), 3.964 (s, 9H, phenyl-OCH3), 7.103 (s, 2H, phenyl-H), 7.169 (s, 2H, phenyl-H) 7.248 (d, 1H, J = 8.5 Hz, quinazoline-H), 7.314 (d, 1H, J = 7.5 Hz, quinazoline-H), 7.429–7.445 (d, 1H, J = 8.0 Hz, quinazoline-H). 13C NMR (125 MHz, DMSO-d6) δ.56.18, 59.56, 60.23, 105.06, 119.63, 125.65, 126.05, 127.12, 129.32,129.65, 129.68, 129.97, 134.13, 134.34, 135.67, 145.96, 164.43, 165.34. MS (m/z): [M+1]: 509.1467.

3.2. Molecular Docking

The molecular docking was performed using Autodock Vina program [38]. The crystal structures of proteins (PDB code 6S9B for EGFR and 4ASD for VEGFR2) were prepared by eliminating unwanted co-crystalized ligands and water molecules using Discovery Studio Visualizer (Accelrys, USA). AutoDock Tools was employed to prepare the input pdbqt files for proteins and ligands and to set the size and the center of the grid box. The size of EGFR active site was set at 14 × 18 × 16 Å coordinates in x, y, and z dimensions and centered to (x = −51.787, y = 21.484, z= −0.026) using 1000 Å spacing. The size of VEGFR2 active site was set at 20 × 20 × 20 Å coordinates in x, y, and z dimensions and centered to (x = −23.756, y = −1.152, z = −11.701) using 1000 Å spacing. PyMol [37] and Discovery Studio Visualizer were used to analyze the binding mode and interaction of ligands with proteins.

3.3. Molecular Dynamic Simulation

All atom 20 ns molecular dynamics (MD) simulations was performed using GROMACS 2018.1 software [39]. OPLS-AA/L force field was used to generate the topology of proteins [40]. The topology and parameter of ligands were generated by the Swissparam server (available at http://www.swissparam.ch/, access on 17 May 2021) [41]. The MD simulations were performed using the previously reported method [42]. Briefly, the system was solvated in cubic box with TIP3P as a water model followed by adding counter ions to neutralize the system. Periodic boundary conditions were used during MD simulation. Energy minimization of system was performed using steepest descent algorithm with tolerance value of 1000 kJ mol−1 nm−1. The system was then equilibrated using NVT and NPT ensembles for 100 ps. Finally, 20 ns production MD was performed for the system, with trajectories generated every 2 femtoseconds (fs) and snapshots saved every 2 picoseconds (PS). Standard analysis was applied to calculate the root mean square deviation (RMSD) and hydrogen bond formation over the simulation time. Standard analysis was applied to calculate the root mean square deviation (RMSD) and hydrogen bond formation over the simulation time using gmx rms and gmx hbond, respectively.

3.4. Antitumor Screening

A primary anticancer assay was performed for two enzymes VEGFR2 and EGFR and 3 human tumor cell line panels, Hela, A549, and MDA, which are related to some neoplastic diseases, like cervical, lung, and breast carcinoma, in accordance with the protocol of the Drug Evaluation Branch, National Cancer Institute, Bethesda, MD [29,30,31,32,33].

4. Conclusions

A new series of 8-methoxy-2-trimethoxyphenyl-3-substituted quinazoline-4(3H)-one was synthesized and assessed for antitumor activity against three cell line panels, HeLa, A549, and MDA, using docetaxel as a reference drug. The most potent antitumor activity was found in compound 6, assessed as a VEGFR2 and EGFR enzyme inhibitor, with (IC50, 98.1 and 106 nM, respectively) relative to docetaxel (IC50, 89.3 and 56.1 nM, respectively) as reference drug. The molecular docking and 20 ns molecular dynamics (MD) simulation results suggest a stable binding of M1–6 within the ATP binding pocket of EGFR and VEGFR2 due to the stable hydrogen bonds formed with the proteins. That makes the results in line with the docking and in vitro results, supporting the notion that this compound might act as a dual EGFR and VEGFR2 tyrosine kinase inhibitor. Strong cytotoxic activity was shown for compounds 2, 6, 10, and 8 against Hela cell line with IC50 values of, 2.13, 2.8, 3.98, and 4.94 µM, respectively compared to docetaxel (IC50, 9.65 µM). Strong cytotoxic activity was shown for compound 11 against A549 cell line (IC50, 4.03 µM) in comparison to docetaxel (IC50, 10.8 µM). A strong cytotoxic activity was shown for compounds 6 and 9 against MDA cell line (IC50, 0.79 and 3.42 µM, respectively) relative to docetaxel (IC50, 3.98 µM).

Author Contributions

Conceptualization, A.S.E.-A.; S.G.A.; and M.A.M.; methodology, A.S.E.-A.; S.G.A., M.A.A. (Mubarak A. Alamri), and M.A.M.; formal analysis, A.S.A., S.G.A., A.H.B., A.S.E.-A., S.M.A.; A.S.A.; and M.A.M.; investigation, M.A.A. (Mubarak A. Alamri); S.M.A.; A.B.A.; A.I.A.; M.A.M.; and A.S.A.; resources, M.A.A (Mubarak A. Alamri); S.M.A.; A.B.A.; A.I.A.; M.A.M.; and M.A.A (Manal A. Alossaimi).; data curation, A.S.E.-A., S.G.A., M.A.A., and M.A.M.; validation: A.H.B.; A.S.A.; A.S.E.-A.; S.M.A.; M.A.A. (Mubarak A. Alamri); and M.A.M.; writing—original draft preparation, A.B.A.; A.I.A., S.M.A.; M.A.A. (Mubarak A. Alamri); and M.A.M; writing—review and editing, A.S.E.-A.; A.H.B.; M.A.A. (Manal A. Alossaimi); and M.A.M.; All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Acknowledgments

This Publication was supported by the Deanship of Scientific Research at Prince Sattam University and the authors would like to express their thanks to Esam R. Rashwan, the Head of the confirmatory diagnostic unit—Vacsera, Cairo, Egypt for performing all biological assays reported in this research.

Conflicts of Interest

The authors declare no conflict of interest.

Sample Availability

Samples of the compounds are not available from the authors.

References

  1. Bavetsias, V.; Jakman, A.L.; Marriott, J.H.; Kimbell, R.; Gibson, W.; Boyle, F.T.; Bisset, G.M. Folate-based inhibitors of thymidylate synthase: Synthesis and antitumor activity of gamma-linked sterically hindered dipeptide analogues of 2-desamino-2-methyl-N10-propargyl-5,8-dideazafolic acid (ICI 198583). J. Med. Chem. 1997, 40, 1495–1510. [Google Scholar] [CrossRef] [PubMed]
  2. Bavetsias, V.; Marriott, J.H.; Melin, C.; Kimbell, R.; Matusiak, Z.S.; Boyle, F.T.; Jakman, A.L. Design and synthesis of Cyclopenta[g]quinazoline-based antifolates as inhibitors of thymidylate synthase and potential antitumor agents. J. Med. Chem. 2000, 43, 1910–1926. [Google Scholar] [CrossRef] [PubMed]
  3. El-Azab, A.S.; Abdel-Aziz, A.A.; Bua, S.; Nocentini, A.; El-Gendy, M.A.; Mohamed, M.A.; Shawer, T.Z.; AlSaif, N.A.; Supuran, C.T. Synthesis of benzensulfonamides linked to quinazoline scaffolds as novel carbonic anhydrase inhibitors. Bioorg. Chem. 2019, 89, 78–90. [Google Scholar] [CrossRef] [PubMed]
  4. de Castro Barbosa, M.L.; Lima, L.M.; Tesch, R.; Sant’Anna CM, R.; Totzke, F.; Kubbutat, M.H.; Schächtele, C.; Laufer, S.A.; Barreiro, E.J. Novel 2-chloro-4-anilino-quinazoline derivatives as EGFR and VEGFR-2 dual inhibitors. Eur. J. Med. Chem. 2014, 71, 1–14. [Google Scholar] [CrossRef]
  5. El-Azab, A.S.; Al-Dhfyan, A.; Abdel-Aziz, A.A.; Abou-Zeid, L.A.; Alkahtani, H.M.; Al-Obaid, A.M.; Al-Gendy, M.A. Synthesis, anticancer and apoptosis-inducing activities of quinazoline-isatin conjugates: Epidermal growth factor receptor-tyrosine kinase assay and molecular docking studies. J. Enzyme Inhib. Med. Chem. 2017, 32, 935–944. [Google Scholar] [CrossRef] [Green Version]
  6. El-Azab, A.S.; Abdel-Aziz, A.A.; Ghabbour, H.A.; Al-Gendy, M.A. Synthesis, in vitro antitumour activity, and molecular docking study of novel 2-substituted mercapto- 3-(3,4,5-trimethoxybenzyl)-4(3H)-quinazolinone analogues. J. Enzyme Inhib. Med. Chem. 2017, 32, 1229–1239. [Google Scholar] [CrossRef] [Green Version]
  7. Mohamed, M.A.; Ayyad, R.R.; Shawer, T.Z.; Alaa, A.-M.; El-Azab, A.S. Synthesis and antitumor evaluation of trimethoxyanilides based on 4 (3H)-quinazolinone scaffolds. Eur. J. Med. Chem. 2016, 112, 106–113. [Google Scholar] [CrossRef]
  8. Al-Suwaidan, I.A.; Alanazi, A.M.; El-Azab, A.S.; Al-Obaid, A.M.; El-Tahir, K.E.; Maarouf, A.R.; Abu El-Enin, M.A.; Abdel-Aziz, A.A. Molecular design, synthesis and biological evaluation of cyclic imides bearing benzenesulfonamide fragment as potential COX-2 inhibitors. Part 2. Bioorg. Med. Chem. Lett. 2013, 23, 2601–2605. [Google Scholar] [CrossRef]
  9. Alaa, A.M.; Abou-Zeid, L.A.; ElTahir, E.H.K.; Mohamed, M.A.; El-Enin, M.A.A.; El-Azab, A.S. Design, synthesis of 2, 3-disubstitued 4 (3H)-quinazolinone derivatives as anti-inflammatory and analgesic agents: COX-1/2 inhibitory activities and molecular docking studies. Bioorg. Med. Chem. 2016, 24, 3818–3828. [Google Scholar]
  10. Abdel-Aziz, A.A.-M.; Abou-Zeid, L.A.; El-Tahir, K.E.H.; Ayyad, R.R.; Magda, A.-A.; El-Azab, A.S. Synthesis, anti-inflammatory, analgesic, COX-1/2 inhibitory activities and molecular docking studies of substituted 2-mercapto-4 (3H)-quinazolinones. Eur. J. Med. Chem. 2016, 121, 410–421. [Google Scholar] [CrossRef]
  11. Alafeefy, A.M.; El-Azab, A.S.; Mohamed, M.A.; Bakhat, M.A.; Abdel-Hamid, S. Synthesis of some new substituted iodoquinazoline derivatives and their antimicrobial screening J. Saudi Chem. Soc. 2011, 15, 319–325. [Google Scholar] [CrossRef] [Green Version]
  12. Alanazi, A.M.; Alaa, A.-M.; Al-Suwaidan, I.A.; Abdel-Hamide, S.G.; Shawer, T.Z.; El-Azab, A.S. Design, synthesis and biological evaluation of some novel substituted quinazolines as antitumor agents. Eur. J. Med. Chem. 2014, 79, 446–454. [Google Scholar] [CrossRef] [PubMed]
  13. Alanazi, A.M.; Al-Suwaidan, I.A.; Alaa, A.-M.; Mohamed, M.A.; El_Morsy, A.M.; El-Azab, A.S. Design synthesis and biological evaluation of some novel substituted 2-mercapto-3-phenethylquinazolines as antitumor agents. Med. Chem. Res. 2013, 22, 5566–5577. [Google Scholar] [CrossRef]
  14. Al-Obaid, A.; Abdel-Hamide, S.; El-Kashef, H.; Abdel-Aziz, A.; El-Azab, A.; Al-Khamees, H.; El-Subbagh, H. Synthesis, in vitro antitumor activity and molecular modelling study of certain 2-thieno-4 (3H)-quinazolinone analogs. Eur. J. Med. Chem. 2009, 44, 2379–2391. [Google Scholar] [CrossRef]
  15. Al-Suwaidan, I.A.; Abdel-Aziz, A.A.-M.; Shawer, T.Z.; Ayyad, R.R.; Alanazi, A.M.; El-Morsy, A.M.; Mohamed, M.A.; Abdel-Aziz, N.I.; El-Sayed, M.A.-A.; El-Azab, A.S. Synthesis, antitumor activity and molecular docking study of some novel 3-benzyl-4(3H) quinazolinone analogues. J. Enzyme Inhib. Med. Chem. 2016, 31, 78–89. [Google Scholar] [CrossRef] [Green Version]
  16. Al-Suwaidan, I.A.; Alanazi, A.M.; Alaa, A.-M.; Mohamed, M.A.; El-Azab, A.S. Design, synthesis and biological evaluation of 2-mercapto-3-phenethylquinazoline bearing anilide fragments as potential antitumor agents: Molecular docking study. Bioorg. Med. Chem. Lett. 2013, 23, 3935–3941. [Google Scholar] [CrossRef] [PubMed]
  17. Aziza, M.; Nassar, M.; AbdelHamide, S.; El-Hakim, A.; El-Azab, A. Synthesis and antimicrobial activities of some new 3-heteroaryl-quinazolin-4-ones. Indian J. Heterocycl. Chem. 1996, 6, 25–30. [Google Scholar]
  18. El-Azab, A.S. Synthesis of some new substituted 2-mercaptoquinazoline analogs as potential antimicrobial agents. Phosph. Sulfur Silicon Related Elem. 2007, 182, 333–348. [Google Scholar] [CrossRef]
  19. El-Azab, A.S.; Abdel-Hamide, S.G.; Sayed-Ahmed, M.M.; Hassan, G.S.; El-Hadiyah, T.M.; Al-Shabanah, O.A.; Al-Deeb, O.A.; El-Subbagh, H. Novel 4(3H)-Quinazolinone Analogues: Synthesis and Anticonvulsant Activity, Med. Chem. Res. 2013, 22, 2815–2827. [Google Scholar]
  20. El-Azab, A.S.; Al-Omar, M.A.; Alaa, A.-M.; Abdel-Aziz, N.I.; Magda, A.-A.; Aleisa, A.M.; Sayed-Ahmed, M.M.; Abdel-Hamide, S.G. Design, synthesis and biological evaluation of novel quinazoline derivatives as potential antitumor agents: Molecular docking study. Eur. J. Med. Chem. 2010, 45, 4188–4198. [Google Scholar] [CrossRef]
  21. El-Azab, A.S.; ElTahir, K.E. Synthesis and anticonvulsant evaluation of some new 2,3,8-trisubstituted-4 (3H)-quinazoline derivatives. Bioorg. Med. Chem. Lett. 2012, 22, 327–333. [Google Scholar] [CrossRef]
  22. El-Azab, A.S.; ElTahir, K.E. Design and synthesis of novel 7-aminoquinazoline derivatives: Antitumor and anticonvulsant activities. Bioorg. Med. Chem. Lett. 2012, 22, 1879–1885. [Google Scholar] [CrossRef] [PubMed]
  23. El-Azab, A.S.; ElTahir, K.E.; Attia, S.M. Synthesis and anticonvulsant evaluation of some novel 4 (3H)-quinazolinones. Mon. Chem.-Chem. Month. 2011, 142, 837–848. [Google Scholar] [CrossRef]
  24. Alanazi, A.M.; Abdel-Aziz, A.A.; Shawer, T.Z.; Ayyad, R.R.; Al-Obaid, A.M.; Al-Agamy, M.H.; Maarouf, A.R.; El-Azab, A.S. Synthesis, antitumor and antimicrobial activity of some new 6-methyl-3-phenyl-4(3H)-quinazolinone analogues: In silico studies. J. Enzyme Inhib. Med. Chem. 2016, 31, 721–735. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  25. Larroque, A.L.; Peori, B.; Williams, C.; Fang, Y.Q.; Qiu, Q.; Rachid, Z.; Jean-Claude, B.J. Synthesis of Water Soluble Bis-triazenoquinazolines: An Unusual Predicted Mode of Binding to the Epidermal Growth Factor Receptor Tyrosine Kinase. J. Chem. Biol. Drug Des. 2008, 71, 374–379. [Google Scholar] [CrossRef]
  26. Amin, K.M.; Georgey, H.H.; Awadallah, F.M. EGFR tyrosine kinase targeted compounds: Synthesis, docking study, and in vitro antitumor activity of some new quinazoline and benzo[d]isothiazole derivatives. J. Med. Chem. Res. 2011, 20, 1042–1053. [Google Scholar] [CrossRef]
  27. Mphahlele, M.J.; Maluleka, M.M.; Mmonw, M.M. Synthesis of Heterocycle-Appended 4-Aminoquinazolines with Antiproliferative Properties and Potential to Inhibit Tyrosine Kinases. In Chemistry for a Clean and Healthy Planet; Springer: Cham, Germany, 2019. [Google Scholar]
  28. Mohamed, M.A.; Alafeefy, A.M.; Abdel Hamid, S.G. Synthesis of Some New Quinazoline Derivatives as Potential Anti-Infective Agents. Life Sci. J. 2014, 11, 108–112. [Google Scholar]
  29. Grever, M.R.; Schepartz, S.A.; Chabner, B.A. The National Cancer Institute: Cancer drug discovery and development program. Semin. Oncol. 1992, 19, 622–638. [Google Scholar]
  30. Monks, A.; Scudiero, D.; Skehan, P.; Shoemaker, R.; Paull, K.; Vistica, D.; Hose, C.; Langley, J.; Cronise, P.; Vaigro-Wolff, A.; et al. Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines. J. Natl. Cancer Inst. 1991, 83, 757–766. [Google Scholar] [CrossRef]
  31. Boyd, M.R.; Paull, K. Some practical considerations and applications of the national cancer institute in vitro anticancer drug discovery screen. Drug Dev. Res. 1995, 34, 91–109. [Google Scholar] [CrossRef]
  32. Shoemaker, R.H. The NCI60 human tumour cell line anticancer drug screen. Nat. Rev. Cancer 2006, 6, 813–823. [Google Scholar] [CrossRef]
  33. Alley, M.C.; Scudiero, D.A.; Monks, A.; Hursey, M.L.; Czerwinski, M.J.; Fine, D.L.; Abbott, B.J.; Mayo, J.G.; Shoemaker, R.H.; Boyd, M.R. Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res. 1988, 48, 589–601. [Google Scholar] [PubMed]
  34. Engelhardt, H.; Böse, D.; Petronczki, M.; Scharn, D.; Bader, G.; Baum, A.; Bergner, A.; Chong, E.; Döbel, S.; Egger, G.; et al. Start selective and rigidify: The discovery path toward a next generation of EGFR tyrosine kinase inhibitors. J. Med. Chem. 2019, 62, 10272–10293. [Google Scholar] [CrossRef] [Green Version]
  35. Bersanelli, M.; Minari, R.; Bordi, P.; Gnetti, L.; Bozzetti, C.; Squadrilli, A.; Lagrasta, C.; Bottarelli, L.; Osipova, G.; Capelletto, E.; et al. L718Q Mutation as New Mechanism of Acquired Resistance to AZD9291 in EGFR -Mutated NSCLC. J. Thorac. Oncol. 2016, 11, e121–e123. [Google Scholar] [CrossRef] [Green Version]
  36. McTigue, M.; Murray, B.W.; Chen, J.H.; Deng, Y.L.; Solowiej, J.; Kania, R.S. Molecular conformations, interactions, and properties associated with drug efficiency and clinical performance among VEGFR TK inhibitors. Proc. Natl. Acad. Sci. USA 2012, 109, 18281–18289. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  37. Delano, W.L. Pymol: An open-source molecular graphics tool. CCP4 Newsl. Protein Crystallogr. 2002, 40, 82–92. [Google Scholar]
  38. Oleg, T.; Olson, A.J. AutoDock Vina: Improving the speed and accuracy of docking with a new scoring function, efficient optimization, and multithreading. J. Comput. Chem. 2010, 31, 455–461. [Google Scholar]
  39. Hess, B.; Kutzner, C.; van der Spoel, D.; Lindahl, E. GROMACS 4:  Algorithms for Highly Efficient, Load-Balanced, and Scalable Molecular Simulation. J. Chem. Theory Comput 2008, 4, 435–447. [Google Scholar] [CrossRef] [Green Version]
  40. Kaminski, G.A.; Friesner, R.A.; Tirado-Rives, J.; Jorgensen, W.L. Evaluation and Reparameterization of the OPLS-AA Force Field for Proteins via Comparison with Accurate Quantum Chemical Calculations on Peptides. J. Phys. Chem. B 2001, 105, 6474–6487. [Google Scholar] [CrossRef]
  41. Zoete, V.; Cuendet, M.A.; Grosdidier, A.; Michielin, O. SwissParam: A fast force field generation tool for small organic molecules. J. Comput. Chem. 2011, 32, 2359–2368. [Google Scholar] [CrossRef] [PubMed]
  42. Alamri, M.A. Pharmacoinformatics and molecular dynamic simulation studies to identify potential small-molecule inhibitors of WNK-SPAK/OSR1 signaling that mimic the RFQV motifs of WNK kinases. Arab. J. Chem. 2020, 13, 5107–5117. [Google Scholar] [CrossRef]
Molecules 26 02992 g001 550
Figure 1. Chemical structure for some quinazoline derivatives used as anticancer drugs, such as nolatrexed and Raltitrexed.
Figure 1. Chemical structure for some quinazoline derivatives used as anticancer drugs, such as nolatrexed and Raltitrexed.
Molecules 26 02992 g001
Molecules 26 02992 g002 550
Figure 2. Reported quinazoline with GI50 µg/mL [15] and designed quinazoline derivatives as antitumor compounds.
Figure 2. Reported quinazoline with GI50 µg/mL [15] and designed quinazoline derivatives as antitumor compounds.
Molecules 26 02992 g002
Molecules 26 02992 sch001 550
Scheme 1. Synthesis of new quinazoline derivatives 2–11.
Scheme 1. Synthesis of new quinazoline derivatives 2–11.
Molecules 26 02992 sch001
Molecules 26 02992 g003 550
Figure 3. Representative graph showing the most active compounds in every cell line, where Hela cell line columns are in blue, A549 cell line columns are in purple, and MDA cell line columns are in white.
Figure 3. Representative graph showing the most active compounds in every cell line, where Hela cell line columns are in blue, A549 cell line columns are in purple, and MDA cell line columns are in white.
Molecules 26 02992 g003
Molecules 26 02992 g004 550
Figure 4. The binding of M6 to EGFR L858R/T790M tyrosine kinase (PDB ID: 6S9B). (A) Surface representation for the binding mode of M6 (green), docetaxel (pink), and co-crystallized ligand compound 1 (white) to EGFR tyrosine kinase. Ribbon representation for the binding interaction of (B) M6 (green) and (C) docetaxel (pink) with EGFR tyrosine kinase. Hydrogen bonds are shown in green dashed lines.
Figure 4. The binding of M6 to EGFR L858R/T790M tyrosine kinase (PDB ID: 6S9B). (A) Surface representation for the binding mode of M6 (green), docetaxel (pink), and co-crystallized ligand compound 1 (white) to EGFR tyrosine kinase. Ribbon representation for the binding interaction of (B) M6 (green) and (C) docetaxel (pink) with EGFR tyrosine kinase. Hydrogen bonds are shown in green dashed lines.
Molecules 26 02992 g004
Molecules 26 02992 g005 550
Figure 5. (A) The backbone RMSD of M6-EGFR complex for 20 ns MD simulation. (B) Total number of molecular hydrogen bonds for the M6-EGFR complex over the simulation time.
Figure 5. (A) The backbone RMSD of M6-EGFR complex for 20 ns MD simulation. (B) Total number of molecular hydrogen bonds for the M6-EGFR complex over the simulation time.
Molecules 26 02992 g005
Molecules 26 02992 g006 550
Figure 6. The binding of M6 to VEGFR2 tyrosine kinase (PDB ID: 4ASD). (A) Surface representation for the binding mode of M6 (green), docetaxel (pink), and co-crystallized sorafenib (yellow) to VEGFR2 tyrosine kinase. Ribbon representation for the binding interaction of (B) M6 (green) and (C) docetaxel (pink) with VEGFR2 tyrosine kinase. Hydrogen bonds are shown in green dashed lines.
Figure 6. The binding of M6 to VEGFR2 tyrosine kinase (PDB ID: 4ASD). (A) Surface representation for the binding mode of M6 (green), docetaxel (pink), and co-crystallized sorafenib (yellow) to VEGFR2 tyrosine kinase. Ribbon representation for the binding interaction of (B) M6 (green) and (C) docetaxel (pink) with VEGFR2 tyrosine kinase. Hydrogen bonds are shown in green dashed lines.
Molecules 26 02992 g006
Molecules 26 02992 g007 550
Figure 7. (A) The backbone RMSD of M6-VEGFR2 complex for 20 ns MD simulation. (B) Total number of molecular hydrogen bonds for the M6-VEGFR2 complex over the simulation time.
Figure 7. (A) The backbone RMSD of M6-VEGFR2 complex for 20 ns MD simulation. (B) Total number of molecular hydrogen bonds for the M6-VEGFR2 complex over the simulation time.
Molecules 26 02992 g007
Table
Table 1. IC50 [nM] values for the cytotoxic effect of compound 6 and docetaxel on enzymes VEGFR2 and EGFR.
Table 1. IC50 [nM] values for the cytotoxic effect of compound 6 and docetaxel on enzymes VEGFR2 and EGFR.
CompoundResults
VEGFR2EGFR
Compound No.CodeMWIC50nMIC50nM
6M636098.1 ± 2.93106 ± 2.22
ReferenceDocetaxel807.8889.3 ± 2.6756.1 ± 1.17
Table
Table 2. IC50 [µM] values of compounds 2–11 and docetaxel.
Table 2. IC50 [µM] values of compounds 2–11 and docetaxel.
Compound No.Group MCytotoxicity IC50 µM
CodeMWHelaA549MDA
4M-135729.8 ± 0.7818 ± 0.54.12 ± 0.1
3M-234366.5 ± 1.7443.6 ± 1.117.4 ± 0.5
11M-350612.0 ± 0.314.03 ± 0.17.06 ± 0.2
2M-43612.13 ± 0.0629.7 ± 0.89.24 ± 0.2
5M-534212.9 ± 0.3426.3 ± 0.74.39 ± 0.1
6M-63602.8 ± 0.0781 ± 2.10.79 ± 0.04
7M-737518.7 ± 0.4946.3 ± 1.29.0 ± 0.2
8M-85154.94 ± 0.1313 ± 0.311.7 ± 0.3
9M-949711.7 ± 0.3119.9 ± 0.53.42 ± 0.1
10M-103563.98 ± 0.112.3 ± 0.37.77 ± 0.2
Ref.Docetaxel807.89.65 ± 0.210.8 ± 0.233.98 ± 0.08
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Altamimi, A.S.; El-Azab, A.S.; Abdelhamid, S.G.; Alamri, M.A.; Bayoumi, A.H.; Alqahtani, S.M.; Alabbas, A.B.; Altharawi, A.I.; Alossaimi, M.A.; Mohamed, M.A. Synthesis, Anticancer Screening of Some Novel Trimethoxy Quinazolines and VEGFR2, EGFR Tyrosine Kinase Inhibitors Assay; Molecular Docking Studies. Molecules 2021, 26, 2992. https://doi.org/10.3390/molecules26102992

AMA Style

Altamimi AS, El-Azab AS, Abdelhamid SG, Alamri MA, Bayoumi AH, Alqahtani SM, Alabbas AB, Altharawi AI, Alossaimi MA, Mohamed MA. Synthesis, Anticancer Screening of Some Novel Trimethoxy Quinazolines and VEGFR2, EGFR Tyrosine Kinase Inhibitors Assay; Molecular Docking Studies. Molecules. 2021; 26(10):2992. https://doi.org/10.3390/molecules26102992

Chicago/Turabian Style

Altamimi, Abdulmalik S., Adel S. El-Azab, Sami G. Abdelhamid, Mubarak A. Alamri, Ashraf H. Bayoumi, Safar M. Alqahtani, Alhumaidi B. Alabbas, Ali I. Altharawi, Manal A. Alossaimi, and Menshawy A. Mohamed. 2021. "Synthesis, Anticancer Screening of Some Novel Trimethoxy Quinazolines and VEGFR2, EGFR Tyrosine Kinase Inhibitors Assay; Molecular Docking Studies" Molecules 26, no. 10: 2992. https://doi.org/10.3390/molecules26102992

Article Metrics

Back to TopTop