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Open AccessArticle

Magnesium–Isotope Fractionation in Chlorophyll-a Extracted from Two Plants with Different Pathways of Carbon Fixation (C3, C4)

1
Faculty of Chemistry, Biological and Chemical Research Centre, University of Warsaw, Zwirki i Wigury 101, 02-093 Warszawa, Poland
2
Chemistry Department, University of Guanajuato, L. de Retana 5, 36000 Guanajuato, Mexico
3
Geological Survey of Israel, 32 Y. Leybowitz st., 9692100 Jerusalem, Israel
*
Authors to whom correspondence should be addressed.
Academic Editors: Zikri Arslan and James Barker
Molecules 2020, 25(7), 1644; https://doi.org/10.3390/molecules25071644
Received: 26 February 2020 / Revised: 26 March 2020 / Accepted: 31 March 2020 / Published: 3 April 2020
Relatively few studies have been focused so far on magnesium–isotope fractionation during plant growth, element uptake from soil, root-to-leaves transport and during chlorophylls biosynthesis. In this work, maize and garden cress were hydroponically grown in identical conditions in order to examine if the carbon fixation pathway (C4, C3, respectively) might have impact on Mg-isotope fractionation in chlorophyll-a. The pigment was purified from plants extracts by preparative reversed phase chromatography, and its identity was confirmed by high-resolution mass spectrometry. The green parts of plants and chlorophyll-a fractions were acid-digested and submitted to ion chromatography coupled through desolvation system to multiple collector inductively coupled plasma-mass spectrometry. Clear preference for heavy Mg-isotopes was found in maize green parts (∆26Mgplant-nutrient 0.65, 0.74 for two biological replicates, respectively) and in chlorophyll-a (∆26Mgchlorophyll-plant 1.51, 2.19). In garden cress, heavy isotopes were depleted in green parts (∆26Mgplant-nutrient (−0.87)–(−0.92)) and the preference for heavy isotopes in chlorophyll-a was less marked relative to maize (∆26Mgchlorophyll-plant 0.55–0.52). The observed effect might be ascribed to overall higher production of energy in form of adenosine triphosphate (ATP), required for carbon fixation in C4 compared to C3, which could reduce kinetic barrier and make equilibrium fractionation prevailing during magnesium incorporation to protoporphyrin ring. View Full-Text
Keywords: Mg-isotope fractionation; chlorophyll; C3 plants; C4 plants; ion chromatography; multiple collector-inductively coupled mass spectrometry Mg-isotope fractionation; chlorophyll; C3 plants; C4 plants; ion chromatography; multiple collector-inductively coupled mass spectrometry
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Wrobel, K.; Karasiński, J.; Tupys, A.; Arroyo Negrete, M.A.; Halicz, L.; Wrobel, K.; Bulska, E. Magnesium–Isotope Fractionation in Chlorophyll-a Extracted from Two Plants with Different Pathways of Carbon Fixation (C3, C4). Molecules 2020, 25, 1644.

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