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Open AccessArticle

Cellular Internalization of Beta-Carotene Loaded Polyelectrolyte Multilayer Capsules by Raman Mapping

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Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, Calea Mănăștur 3-5, 400372 Cluj-Napoca, Romania
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Faculty of Physics, Babeș-Bolyai University, Kogalniceanu 1, 400084 Cluj-Napoca, Romania
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Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine, Calea Mănăștur 3-5, 400372 Cluj-Napoca, Romania
4
National Institute for Research and Development of Isotopic and Molecular Technologies, Donath 67-103, 400293 Cluj-Napoca, Romania
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Authors to whom correspondence should be addressed.
Molecules 2020, 25(7), 1477; https://doi.org/10.3390/molecules25071477
Received: 18 February 2020 / Revised: 20 March 2020 / Accepted: 21 March 2020 / Published: 25 March 2020
Raman mapping is becoming a very useful tool in investigating cells and cellular components, as well as bioactive molecules intracellularly. In this study, we have encapsulated beta-carotene using a layer-by-layer technique, as a way to enhance its stability and bioavailability. Further, we have used Raman mapping to characterize the as-obtained capsules and monitor their uptake by the human retinal epithelial D407 cells. We were able to successfully map the beta-carotene distribution inside the capsules, to localize the capsules intracellularly, and distinguish between capsules and other cellular components. View Full-Text
Keywords: beta-carotene; Raman mapping; polyelectrolyte multilayer; microcapsules; encapsulation beta-carotene; Raman mapping; polyelectrolyte multilayer; microcapsules; encapsulation
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Leopold, L.F.; Marișca, O.; Oprea, I.; Rugină, D.; Suciu, M.; Nistor, M.; Tofană, M.; Leopold, N.; Coman, C. Cellular Internalization of Beta-Carotene Loaded Polyelectrolyte Multilayer Capsules by Raman Mapping. Molecules 2020, 25, 1477.

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