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Article

Good Vibrations: Structural Remodeling of Maturing Yeast Pre-40S Ribosomal Particles Followed by Cryo-Electron Microscopy

1
Laboratoire de Biologie Moléculaire Eucaryote, Centre de Biologie Intégrative, Université de Toulouse, CNRS, UPS, 118 route de Narbonne, 31062 Toulouse CEDEX, France
2
Institut de Pharmacologie et Biologie Structurale, Université de Toulouse, CNRS, UPS, 205 route de Narbonne, 31062 Toulouse CEDEX, France
*
Authors to whom correspondence should be addressed.
Present address: Institute of Structural and Molecular Biology, Birkbeck, University of London, Malet Street, London WC1E 7HX, UK.
Present address: Department of Life Sciences, Sir Ernst Chain Building, Imperial College London, London SW7 2BU, UK.
Academic Editor: Quentin Vicens
Molecules 2020, 25(5), 1125; https://doi.org/10.3390/molecules25051125
Received: 20 January 2020 / Revised: 24 February 2020 / Accepted: 27 February 2020 / Published: 3 March 2020
(This article belongs to the Special Issue Frontiers in RNA Structure)
Assembly of eukaryotic ribosomal subunits is a very complex and sequential process that starts in the nucleolus and finishes in the cytoplasm with the formation of functional ribosomes. Over the past few years, characterization of the many molecular events underlying eukaryotic ribosome biogenesis has been drastically improved by the “resolution revolution” of cryo-electron microscopy (cryo-EM). However, if very early maturation events have been well characterized for both yeast ribosomal subunits, little is known regarding the final maturation steps occurring to the small (40S) ribosomal subunit. To try to bridge this gap, we have used proteomics together with cryo-EM and single particle analysis to characterize yeast pre-40S particles containing the ribosome biogenesis factor Tsr1. Our analyses lead us to refine the timing of the early pre-40S particle maturation steps. Furthermore, we suggest that after an early and structurally stable stage, the beak and platform domains of pre-40S particles enter a “vibrating” or “wriggling” stage, that might be involved in the final maturation of 18S rRNA as well as the fitting of late ribosomal proteins into their mature position. View Full-Text
Keywords: ribosome assembly; small ribosomal subunit; bottom-up proteomics; cryo-EM; single particle analysis ribosome assembly; small ribosomal subunit; bottom-up proteomics; cryo-EM; single particle analysis
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MDPI and ACS Style

Shayan, R.; Rinaldi, D.; Larburu, N.; Plassart, L.; Balor, S.; Bouyssié, D.; Lebaron, S.; Marcoux, J.; Gleizes, P.-E.; Plisson-Chastang, C. Good Vibrations: Structural Remodeling of Maturing Yeast Pre-40S Ribosomal Particles Followed by Cryo-Electron Microscopy. Molecules 2020, 25, 1125. https://doi.org/10.3390/molecules25051125

AMA Style

Shayan R, Rinaldi D, Larburu N, Plassart L, Balor S, Bouyssié D, Lebaron S, Marcoux J, Gleizes P-E, Plisson-Chastang C. Good Vibrations: Structural Remodeling of Maturing Yeast Pre-40S Ribosomal Particles Followed by Cryo-Electron Microscopy. Molecules. 2020; 25(5):1125. https://doi.org/10.3390/molecules25051125

Chicago/Turabian Style

Shayan, Ramtin; Rinaldi, Dana; Larburu, Natacha; Plassart, Laura; Balor, Stéphanie; Bouyssié, David; Lebaron, Simon; Marcoux, Julien; Gleizes, Pierre-Emmanuel; Plisson-Chastang, Célia. 2020. "Good Vibrations: Structural Remodeling of Maturing Yeast Pre-40S Ribosomal Particles Followed by Cryo-Electron Microscopy" Molecules 25, no. 5: 1125. https://doi.org/10.3390/molecules25051125

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