Green Synthesis of Ag-MnO2 Nanoparticles using Chelidonium majus and Vinca minor Extracts and Their In Vitro Cytotoxicity
by
Alexandra Ciorîță 1,2, Maria Suciu 1,2
, Sergiu Macavei 2,3, Irina Kacso 2, Ildiko Lung 2, Maria-Loredana Soran 2,* and Marcel Pârvu 1
Alexandra Ciorîță 1,2, Maria Suciu 1,2, Sergiu Macavei 2,3, Irina Kacso 2, Ildiko Lung 2, Maria-Loredana Soran 2,* and Marcel Pârvu 1
1
Babeș-Bolyai University, Faculty of Biology and Geology, 44 Republicii, 400015 Cluj-Napoca, Romania
2
National Institute for Research and Development of Isotopic and Molecular Technologies, 400293 Cluj-Napoca, Romania
3
Technical University, Faculty of Mechanical Engineering, Department of Mechatronics and Machine Dynamics, 400641 Cluj-Napoca, Romania
*
Author to whom correspondence should be addressed.
Molecules 2020, 25(4), 819; https://doi.org/10.3390/molecules25040819 (registering DOI)
Received: 16 January 2020 / Revised: 11 February 2020 / Accepted: 11 February 2020 / Published: 13 February 2020
Medicinal plants are often used as reducing agents to prepare metal nanoparticles through green-synthesis due to natural compounds and their potential as chemotherapeutic drugs. Thus, three types of eco-friendly Ag-MnO2 nanoparticles (Ag-MnO2NPs) were synthesized using C. majus (CmNPs), V. minor (VmNPs), and a 1:1 mixture of the two extracts (MNPs). These NPs were characterized using S/TEM, EDX, XRD, and FTIR methods, and their biological activity was assessed in vitro on normal keratinocytes (HaCaT) and skin melanoma cells (A375). All synthesized NPs had manganese oxide in the middle, and silver oxide and plant extract on the exterior. The NPs had different forms (polygonal, oval, and spherical), uniformly distributed, with crystalline structures and different sizes (9.3 nm for MNPs; 10 nm for VmNPs, and 32.4 nm for CmNPs). The best results were obtained with VmNPs, which reduced the viability of A375 cells up 38.8% and had a moderate cytotoxic effect on HaCaT (46.4%) at concentrations above 500 µg/mL. At the same concentrations, CmNPs had a rather proliferative effect, whereas MNPs negatively affected both cell lines. For the first time, this paper proved the synergistic action of the combined C. majus and V. minor extracts to form small and uniformly distributed Ag-MnO2NPs with high potential for selective treatments.
View Full-Text
▼
Show Figures
Figure 1
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
MDPI and ACS Style
Ciorîță, A.; Suciu, M.; Macavei, S.; Kacso, I.; Lung, I.; Soran, M.-L.; Pârvu, M. Green Synthesis of Ag-MnO2 Nanoparticles using Chelidonium majus and Vinca minor Extracts and Their In Vitro Cytotoxicity. Molecules 2020, 25, 819.
Show more citation formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.
