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Open AccessArticle

Bioorthogonal Labeling Reveals Different Expression of Glycans in Mouse Hippocampal Neuron Cultures during Their Development

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3B’s Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, Barco, 4805-017 Guimarães, Portugal
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Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal
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ICVS/3B’s—PT Government Associate Laboratory, 4710-057 Braga, Guimarães, Portugal
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The Discoveries Centre for Regenerative and Precision Medicine, Headquarters at University of Minho, Avepark, Barco, 4805-017 Guimarães, Portugal
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Authors to whom correspondence should be addressed.
Present Address: Center for Brain Immunology and Glia, Department of Neuroscience, School of Medicine, University of Virginia, Charlottesville, VA 22908, USA.
Academic Editor: Vito Ferro
Molecules 2020, 25(4), 795; https://doi.org/10.3390/molecules25040795 (registering DOI)
Received: 9 January 2020 / Revised: 7 February 2020 / Accepted: 10 February 2020 / Published: 12 February 2020
(This article belongs to the Special Issue Glycans in Design and Synthesis of Biofunctional Materials)
The expression of different glycans at the cell surface dictates cell interactions with their environment and other cells, being crucial for the cell fate. The development of the central nervous system is associated with tremendous changes in the cell glycome that is tightly regulated. Herein, we have employed biorthogonal Cu-free click chemistry to image temporal distribution of different glycans in live mouse hippocampal neurons during their maturation in vitro. We show development-dependent glycan patterns with increased fucose and decreased mannose expression at the end of the maturation process. We also demonstrate that this approach is biocompatible and does not affect glycan transport although it relies on an administration of modified glycans. The applicability of this strategy to tissue sections unlocks new opportunities to study the glycan dynamics under more complex physiological conditions. View Full-Text
Keywords: glycosylation; biorthogonal chemistry; neuronal development; imaging glycosylation; biorthogonal chemistry; neuronal development; imaging
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Soares da Costa, D.; Sousa, J.C.; Dá Mesquita, S.; Petkova-Yankova, N.I.; Marques, F.; Reis, R.L.; Sousa, N.; Pashkuleva, I. Bioorthogonal Labeling Reveals Different Expression of Glycans in Mouse Hippocampal Neuron Cultures during Their Development. Molecules 2020, 25, 795.

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