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Open AccessReview

Digital PCR as an Emerging Tool for Monitoring of Microbial Biodegradation

The Northern Region Persistent Organic Pollution (NRPOP) Control Laboratory, Faculty of Engineering and Applied Science, Memorial University of Newfoundland, St. John’s, NL A1B 3X5, Canada
Authors to whom correspondence should be addressed.
Molecules 2020, 25(3), 706;
Received: 27 December 2019 / Revised: 3 February 2020 / Accepted: 4 February 2020 / Published: 6 February 2020
(This article belongs to the Special Issue Biodegradation of Conventional and Emerging Pollutants)
Biodegradation of contaminants is extremely complicated due to unpredictable microbial behaviors. Monitoring of microbial biodegradation drives us to determine (1) the amounts of specific degrading microbes, (2) the abundance, and (3) expression level of relevant functional genes. To this endeavor, the cultivation independent polymerase chain reaction (PCR)-based monitoring technique develops from endpoint PCR, real-time quantitative PCR, and then into novel digital PCR. In this review, we introduce these three categories of PCR techniques and summarize the timely applications of digital PCR and its superiorities than qPCR for biodegradation monitoring. Digital PCR technique, emerging as the most accurately absolute quantification method, can serve as the most promising and robust tool for monitoring of microbial biodegradation. View Full-Text
Keywords: biodegradation; digital PCR; monitoring; emerging contaminants biodegradation; digital PCR; monitoring; emerging contaminants
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MDPI and ACS Style

Cao, Y.; Yu, M.; Dong, G.; Chen, B.; Zhang, B. Digital PCR as an Emerging Tool for Monitoring of Microbial Biodegradation. Molecules 2020, 25, 706.

AMA Style

Cao Y, Yu M, Dong G, Chen B, Zhang B. Digital PCR as an Emerging Tool for Monitoring of Microbial Biodegradation. Molecules. 2020; 25(3):706.

Chicago/Turabian Style

Cao, Yiqi; Yu, Miao; Dong, Guihua; Chen, Bing; Zhang, Baiyu. 2020. "Digital PCR as an Emerging Tool for Monitoring of Microbial Biodegradation" Molecules 25, no. 3: 706.

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