Next Article in Journal
Trityl-Containing Alcohols—An Efficient Chirality Transmission Process from Inductor to the Stereodynamic Propeller and their Solid-State Structural Diversity
Next Article in Special Issue
Assessment of Biodegradation Efficiency of Polychlorinated Biphenyls (PCBs) and Petroleum Hydrocarbons (TPH) in Soil Using Three Individual Bacterial Strains and Their Mixed Culture
Previous Article in Journal
Z-Ajoene Inhibits Growth of Colon Cancer by Promotion of CK1α Dependent β-Catenin Phosphorylation
Previous Article in Special Issue
Hydrocarbon Removal by Two Differently Developed Microbial Inoculants and Comparing Their Actions with Biostimulation Treatment
Open AccessReview

Digital PCR as an Emerging Tool for Monitoring of Microbial Biodegradation

The Northern Region Persistent Organic Pollution (NRPOP) Control Laboratory, Faculty of Engineering and Applied Science, Memorial University of Newfoundland, St. John’s, NL A1B 3X5, Canada
*
Authors to whom correspondence should be addressed.
Molecules 2020, 25(3), 706; https://doi.org/10.3390/molecules25030706
Received: 27 December 2019 / Revised: 3 February 2020 / Accepted: 4 February 2020 / Published: 6 February 2020
(This article belongs to the Special Issue Biodegradation of Conventional and Emerging Pollutants)
Biodegradation of contaminants is extremely complicated due to unpredictable microbial behaviors. Monitoring of microbial biodegradation drives us to determine (1) the amounts of specific degrading microbes, (2) the abundance, and (3) expression level of relevant functional genes. To this endeavor, the cultivation independent polymerase chain reaction (PCR)-based monitoring technique develops from endpoint PCR, real-time quantitative PCR, and then into novel digital PCR. In this review, we introduce these three categories of PCR techniques and summarize the timely applications of digital PCR and its superiorities than qPCR for biodegradation monitoring. Digital PCR technique, emerging as the most accurately absolute quantification method, can serve as the most promising and robust tool for monitoring of microbial biodegradation. View Full-Text
Keywords: biodegradation; digital PCR; monitoring; emerging contaminants biodegradation; digital PCR; monitoring; emerging contaminants
Show Figures

Figure 1

MDPI and ACS Style

Cao, Y.; Yu, M.; Dong, G.; Chen, B.; Zhang, B. Digital PCR as an Emerging Tool for Monitoring of Microbial Biodegradation. Molecules 2020, 25, 706. https://doi.org/10.3390/molecules25030706

AMA Style

Cao Y, Yu M, Dong G, Chen B, Zhang B. Digital PCR as an Emerging Tool for Monitoring of Microbial Biodegradation. Molecules. 2020; 25(3):706. https://doi.org/10.3390/molecules25030706

Chicago/Turabian Style

Cao, Yiqi; Yu, Miao; Dong, Guihua; Chen, Bing; Zhang, Baiyu. 2020. "Digital PCR as an Emerging Tool for Monitoring of Microbial Biodegradation" Molecules 25, no. 3: 706. https://doi.org/10.3390/molecules25030706

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop