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Volume 25
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10.3390/molecules25245930
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Article

Optimization of Bioprocess Extraction of Poria cocos Polysaccharide (PCP) with Aspergillus niger β-Glucanase and the Evaluation of PCP Antioxidant Property

by
Peng Wu
1,
Hongyuan Tan
1,
Jianfeng Zhan
1,
Weixin Wang
1,
Ting Hu
1,* and
Shiming Li
1,2,*
1
Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountain, Hubei Zhongke Industrial Technology Research Institute, Huanggang Normal University, Huanggang 438000, China
2
Department of Food Science, Rutgers University, New Brunswick, NJ 07102, USA
*
Authors to whom correspondence should be addressed.
Academic Editors: Ryszard Amarowicz and Adriano Costa de Camargo
Molecules 2020, 25(24), 5930; https://doi.org/10.3390/molecules25245930
Received: 24 November 2020 / Revised: 7 December 2020 / Accepted: 9 December 2020 / Published: 15 December 2020
(This article belongs to the Special Issue 25th Anniversary of Molecules—Advances in Food Chemistry, Nutraceuticals, Functional Foods, and Dietary Bioactives: Celebrating the Lifetime Achievements of Professor Fereidoon Shahidi)
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Abstract

Poria cocos mushroom is widely used as a food and an herb in East Asian and other countries due to its high nutritional value. Research has demonstrated that Poria cocos polysaccharides (PCP) are the major bioactives and possess antioxidation, anti-inflammation, immunoregulation, and other health promoting properties. However, the efficient preparation of PCP has been a challenge, particularly in large scale for industry. Herein, we investigated the biotransformation of PCP from Poria cocos, catalyzed by β-glucanase from Aspergillus niger and focused on optimizing the most four influencing parameters: Temperature, time, pH, and enzyme dosage in this study. After numerous optimizations with the assistance of response surface optimization methodology, we have established that the optimal conditions for the biotransformation PCP preparation were as following: Enzymolysis temperature 60 °C, time 120 min, pH 5.0 and enzyme dose 20 mL. Under these conditions, the extraction yield of PCP reached as high as 12.8%. In addition, the antioxidant activities of PCP were evaluated by reducing power assay and 1,1-diphenyl-2-picryl-hydrazyl, superoxide anion, and hydroxyl radicals scavenging assays. Resulting data showed that PCP presented outstanding antioxidant capacity. Thus, these findings indicate that PCP could be produced as a natural antioxidant for further development. View Full-Text
Keywords: Poria cocos polysaccharide; biotransformation; β-glucanase; optimal processing conditions; response surface method; antioxidant activity Poria cocos polysaccharide; biotransformation; β-glucanase; optimal processing conditions; response surface method; antioxidant activity
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
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MDPI and ACS Style

Wu, P.; Tan, H.; Zhan, J.; Wang, W.; Hu, T.; Li, S. Optimization of Bioprocess Extraction of Poria cocos Polysaccharide (PCP) with Aspergillus niger β-Glucanase and the Evaluation of PCP Antioxidant Property. Molecules 2020, 25, 5930. https://doi.org/10.3390/molecules25245930

AMA Style

Wu P, Tan H, Zhan J, Wang W, Hu T, Li S. Optimization of Bioprocess Extraction of Poria cocos Polysaccharide (PCP) with Aspergillus niger β-Glucanase and the Evaluation of PCP Antioxidant Property. Molecules. 2020; 25(24):5930. https://doi.org/10.3390/molecules25245930

Chicago/Turabian Style

Wu, Peng, Hongyuan Tan, Jianfeng Zhan, Weixin Wang, Ting Hu, and Shiming Li. 2020. "Optimization of Bioprocess Extraction of Poria cocos Polysaccharide (PCP) with Aspergillus niger β-Glucanase and the Evaluation of PCP Antioxidant Property" Molecules 25, no. 24: 5930. https://doi.org/10.3390/molecules25245930

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