Next Article in Journal
Micro-Fibrillated Cellulose in Adhesive Systems for the Production of Wood-Based Panels
Next Article in Special Issue
Phytochemicals and Antioxidant Activity in Oat-Buckwheat Dough and Cookies with Added Spices or Herbs
Previous Article in Journal
The Circular Life of Human CD38: From Basic Science to Clinics and Back
Article

Phenolic Acids-Rich Fractions from Agaricus bitorguis (Quél.) Sacc. Chaidam ZJU-CDMA-12 Mycelia Modulate Hypoxic Stress on Hypoxia-Damaged PC12 Cells

by 1,†, 2,†, 3, 4 and 1,*
1
Department of Food Science and Nutrition, Zhejiang University, Hangzhou 310058, China
2
Department of Cell Biology, School of Medicine, Johns Hopkins University, Baltimore, MD 21205, USA
3
Agriculture and Animal Husbandry College, Qinghai University, Xining 810016, China
4
Institute of Quality and Standard for Agriculture Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Molecules 2020, 25(20), 4845; https://doi.org/10.3390/molecules25204845
Received: 16 September 2020 / Revised: 10 October 2020 / Accepted: 17 October 2020 / Published: 21 October 2020
Hypoxia is a common pathological process in various clinical diseases. However, there is still a lack of effective anti-hypoxia active substances. Agaricus bitorguis (Quél.) Sacc Chaidam (ABSC) is a rare wild edible macrofungus that grows underground at high altitudes. Herein, intracellular phenolic acids-rich fractions (IPA) were extracted from ABSC ZJU-CDMA-12, and the structural characterization and anti-hypoxia activity of IPA on PC12 cells were elucidated as well. The results of HPLC-Q-TOF-MS illustrated that five kinds of IPA were isolated from ABSC, including (−)-epicatechin gallate, arabelline, yunnaneic acid D, 2′-O-p-hydroxybenzoyl-6′-O-trans-caffeoylgardoside,4′-O-methylgallocatechin-(4->8)-4′-O-methylepigallocatechin. IPA extracted from ABSC proved to show anti-hypoxia activity on hypoxia-damaged PC12 cells. Hypoxia enhanced reactive oxygen species (ROS) generation and reduced the mitochondrial membrane potential (ΔΨm) in PC12 cells, resulting in the inhibition of survival and induction of apoptosis in PC12 cells. Measurements of 100 μg/mL and 250 μg/mL IPA could significantly reduce hypoxia-induced damage in PC12 cells by decreasing overproduced intracellular ROS, improving ΔΨm, and reducing cell apoptosis rate. Our findings indicated that the IPA from ABSC potentially could be used as novel bioactive components applied to anti-hypoxia functional foods or medicines. View Full-Text
Keywords: intracellular phenolic acids; Agaricus bitorquis (QuéL.) Sacc. Chaidam; structure characterization; anti-hypoxia activity intracellular phenolic acids; Agaricus bitorquis (QuéL.) Sacc. Chaidam; structure characterization; anti-hypoxia activity
Show Figures

Graphical abstract

MDPI and ACS Style

Lu, H.; Jiao, Z.; Jiao, Y.; Wang, W.; Chen, Q. Phenolic Acids-Rich Fractions from Agaricus bitorguis (Quél.) Sacc. Chaidam ZJU-CDMA-12 Mycelia Modulate Hypoxic Stress on Hypoxia-Damaged PC12 Cells. Molecules 2020, 25, 4845. https://doi.org/10.3390/molecules25204845

AMA Style

Lu H, Jiao Z, Jiao Y, Wang W, Chen Q. Phenolic Acids-Rich Fractions from Agaricus bitorguis (Quél.) Sacc. Chaidam ZJU-CDMA-12 Mycelia Modulate Hypoxic Stress on Hypoxia-Damaged PC12 Cells. Molecules. 2020; 25(20):4845. https://doi.org/10.3390/molecules25204845

Chicago/Turabian Style

Lu, Hongyun, Zhihua Jiao, Yingchun Jiao, Wei Wang, and Qihe Chen. 2020. "Phenolic Acids-Rich Fractions from Agaricus bitorguis (Quél.) Sacc. Chaidam ZJU-CDMA-12 Mycelia Modulate Hypoxic Stress on Hypoxia-Damaged PC12 Cells" Molecules 25, no. 20: 4845. https://doi.org/10.3390/molecules25204845

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop