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Open AccessArticle

Phytochemical Evaluation of Tinctures and Essential Oil Obtained from Satureja montana Herb

Department of Drug Technology and Biopharmacy, Danylo Halytsky Lviv National Medical University, 79010 Lviv, Ukraine
Department of Analytical and Ecological Chemistry, University of Opole, 45-052 Opole, Poland
Department of Pharmacognosy and Medical Botany, I. Horbachevsky Ternopil National Medical University, 46001 Ternopil, Ukraine
Sector of Mobilization and Conservation of Plant Resources of the Rice Institute of the NAAS, Plodove, Kherson region, 74992 Kherson, Ukraine
Author to whom correspondence should be addressed.
Academic Editor: Nawaf Al-Maharik
Molecules 2020, 25(20), 4763;
Received: 14 September 2020 / Revised: 2 October 2020 / Accepted: 11 October 2020 / Published: 16 October 2020
Winter Savory (Satureja montana L.) has been used in traditional medicine and as a spice or natural food preservative in the Mediterranean region for centuries. In this paper, some technological and analytical aspects of the S. montana tinctures development and an evaluation of the essential oil composition are provided. The total phenolic and flavonoid contents and phenolic compounds profile analyzed spectrophotometrically and by high-performance thin-layer chromatography (HPTLC), respectively, were evaluated in the developed tinctures. The results showed that the tinctures prepared from the S. montana herb by maceration or remaceration are rich in polyphenols, and there is an influence of the technological factors (particle size and extraction mode) on the total phenolic and flavonoid contents. Caffeic, rosmarinic, and chlorogenic acids, (–)-catechin and rutin were identified in the tinctures using the HPTLC method. p-Thymol (81.79%) revealed by gas chromatography-mass spectrometry (GC-MS) was the predominant compound of the essential oil of this plant. Thus, the high contents of polyphenols and flavonoids in the developed tinctures and p-thymol among the volatile components of the S. montana essential oil could indicate the promising antioxidant and antimicrobial properties of these herbal preparations. The obtained results are a ground for the organization of the manufacture of the S. montana tincture and essential oil with the purpose of performing preclinical studies. View Full-Text
Keywords: Satureja montana; tincture; polyphenols; flavonoids; essential oil Satureja montana; tincture; polyphenols; flavonoids; essential oil
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Hudz, N.; Makowicz, E.; Shanaida, M.; Białoń, M.; Jasicka-Misiak, I.; Yezerska, O.; Svydenko, L.; Wieczorek, P.P. Phytochemical Evaluation of Tinctures and Essential Oil Obtained from Satureja montana Herb. Molecules 2020, 25, 4763.

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