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Article

The Chemical Composition of Brazilian Green Propolis and Its Protective Effects on Mouse Aortic Endothelial Cells against Inflammatory Injury

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College of Animal Science (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, China
2
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China
3
Bee Product Processing and Application Research Center of the Ministry of Education, Fuzhou 350002, China
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College of Plant Protection, Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China
*
Author to whom correspondence should be addressed.
Academic Editor: Soraia I. Falcão
Molecules 2020, 25(20), 4612; https://doi.org/10.3390/molecules25204612
Received: 5 September 2020 / Revised: 28 September 2020 / Accepted: 3 October 2020 / Published: 10 October 2020
Propolis has a very complex composition, with antibacterial, anti-inflammatory and other properties. To determine the composition of ethanol extracts of Brazilian green propolis (EEP-B) and their protective effect on mouse aortic endothelial cells (MAECs), the chemical composition of EEP-B was analysed by UPLC/Q-TOF-MS/MS, and the protective effect of EEP-B on the proliferation of lipopolysaccharide (LPS)-induced MAECs was determined by Cell Counting Kit-8 (CCK-8) assays. The protein levels of inflammatory cytokines tumour necrosis factor-α (TNF-α) and interleukin- 6 (IL-6) were measured by enzyme-linked immunosorbent assay (ELISA), and ICAM-1, VCAM-1 and MCP-1 expressions were analysed by western blotting. The results showed that a total of 24 compounds belonging to cinnamic acids and flavonoids, including 3,5-diisopentenyl-4-hydroxycinnamic acid (artepillin C), kaempferide, 3-isoprenyl p-coumaric acid, pinocembrin and 4′-methoxy pinobanksin, were identified in EEP-B. Among them, a new component, suggested to be 5-isoprenyl caffeic acid p-coumaric acid ester, was reported for the first time. The LPS-induced levels of TNF-α, IL-6, ICAM-1, VCAM-1 and MCP-1 were downregulated in response to 5, 10 and 20 μg/mL EEP-B. This study revealed that EEP-B could reduce LPS-induced inflammatory reactions, improve cell survival, and protect MAECs by regulating ICAM-1, VCAM-1 and MCP-1 expression. These findings could provide a theoretical basis for MAEC treatment using EEP-B. View Full-Text
Keywords: Brazilian green propolis; chemical composition; mouse aortic endothelial cells; anti-inflammatory Brazilian green propolis; chemical composition; mouse aortic endothelial cells; anti-inflammatory
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MDPI and ACS Style

Xu, X.; Yang, B.; Wang, D.; Zhu, Y.; Miao, X.; Yang, W. The Chemical Composition of Brazilian Green Propolis and Its Protective Effects on Mouse Aortic Endothelial Cells against Inflammatory Injury. Molecules 2020, 25, 4612. https://doi.org/10.3390/molecules25204612

AMA Style

Xu X, Yang B, Wang D, Zhu Y, Miao X, Yang W. The Chemical Composition of Brazilian Green Propolis and Its Protective Effects on Mouse Aortic Endothelial Cells against Inflammatory Injury. Molecules. 2020; 25(20):4612. https://doi.org/10.3390/molecules25204612

Chicago/Turabian Style

Xu, Xiaolan, Bo Yang, Danfeng Wang, Yuxuan Zhu, Xiaoqing Miao, and Wenchao Yang. 2020. "The Chemical Composition of Brazilian Green Propolis and Its Protective Effects on Mouse Aortic Endothelial Cells against Inflammatory Injury" Molecules 25, no. 20: 4612. https://doi.org/10.3390/molecules25204612

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