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Open AccessArticle

Bioaffinity Fishing Procedure Using Secretory Phospholipase A2 for Screening for Bioactive Components: Modulation of Pharmacological Effect Induced by sPLA2 from Crotalus durissus terrificus by Hispidulin from Moquiniastrum floribundum

1
BIOMOLPEP group, Instituto de Biociências, UNESP, Campus do Litoral Paulista, São Vicente CEP 11380-972, São Paulo 11380-972, Brazil
2
Departamento de Botânica, Instituto de Biociências, Universidade de São Paulo, São Paulo CEP 05508-090, Brazil
3
LABIMES, Instituto de Biociências, UNESP, Campus do Litoral Paulista, São Vicente CEP 11380-972, São Paulo 11380-972, Brazil
*
Author to whom correspondence should be addressed.
Academic Editor: Maria da Graça Costa G. Miguel
Molecules 2020, 25(2), 282; https://doi.org/10.3390/molecules25020282
Received: 30 November 2019 / Revised: 2 January 2020 / Accepted: 6 January 2020 / Published: 9 January 2020
(This article belongs to the Special Issue Anti-Inflammatory Activity of Natural Products)
Bioaffinity capturing of molecules allows the discovery of bioactive compounds and decreases the need for various stages in the natural compound isolation process. Despite the high selectivity of this technique, the screening and identification methodology depends on the presence of a protein to capture potential ligands. However, some proteins, such as snake secretory phospholipase A2 (sPLA2), have never been investigated using this approach. The purpose of this study was to evaluate the use of a new method for screening natural compounds using a bioaffinity-guided ultrafiltration method on Crotalus durissus terrificus sPLA2 followed by HPLC-MS to identify the compounds, and this method could be used to discover new anti-inflammatory compounds from the various organisms originating from biodiversity. Different extracts were selected to evaluate their ability to inhibit sPLA2 activity. The extracts were incubated with sPLA2 and the resulting mixture was ultrafiltrated to elute unbound components. The resulting compounds were identified by HPLC-MS. We identified hispidulin as one of the components present in the Moquiniastrum floribundum leaf and evaluated the ability of this isolated compound to neutralize the inflammatory activity of sPLA2 from Crotalus durissus terrificus. View Full-Text
Keywords: ultrafiltration; anti-inflammatory; natural compounds; bioaffinity ultrafiltration; anti-inflammatory; natural compounds; bioaffinity
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MDPI and ACS Style

dos Santos Junior, A.B.; Tamayose, C.I.; Ferreira, M.J.P.; Belchor, M.N.; Costa, C.R.C.; de Oliveira, M.A.; Toyama, M.H. Bioaffinity Fishing Procedure Using Secretory Phospholipase A2 for Screening for Bioactive Components: Modulation of Pharmacological Effect Induced by sPLA2 from Crotalus durissus terrificus by Hispidulin from Moquiniastrum floribundum. Molecules 2020, 25, 282.

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