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Screening of Lactobacillus plantarum subsp. plantarum with Potential Probiotic Activities for Inhibiting ETEC K88 in Weaned Piglets

1
Henan Key Lab Ion Beam Bioengineering, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450052, China
2
School of Physics and Microelectronics, Zhengzhou University, Zhengzhou 450052, China
*
Author to whom correspondence should be addressed.
Molecules 2020, 25(19), 4481; https://doi.org/10.3390/molecules25194481
Received: 14 August 2020 / Revised: 18 September 2020 / Accepted: 25 September 2020 / Published: 29 September 2020
(This article belongs to the Section Chemical Biology)
For screening excellent lactic acid bacteria (LAB) strains to inhibit enterotoxigenic Escherichia coli (ETEC) K88, inhibitory activities of more than 1100 LAB strains isolated from different materials, and kept in the lab, were evaluated in this study. Nine strains with inhibition zones, at least 22.00 mm (including that of a hole puncher, 10.00 mm), and good physiological and biochemical characteristics identified by 16S DNA gene sequencing and recA gene multiple detection, were assigned to Lactobacillus (L.) plantarum subsp. plantarum (5), L. fermentum (1), L. reuteri (1), Weissella cibaria (1) and Enterococcus faecalis (1), respectively. As investigated for their tolerance abilities and safety, only strain ZA3 possessed high hydrophobicity and auto-aggregation abilities, had high survival rate in low pH, bile salt environment, and gastrointestinal (GI) fluids, was sensitive to ampicillin, and resistant to norfloxacin and amikacin, without hemolytic activity, and did not carry antibiotic resistance genes, but exhibited broad spectrum activity against a wide range of microorganisms. Antibacterial substance may attribute to organic acids, especially lactic acid and acetic acid. The results indicated that the selected strain L. plantarum subsp. plantarum ZA3 could be considered a potential probiotic to inhibit ETEC K88 in weaned piglets for further research. View Full-Text
Keywords: L. plantarum subsp. plantarum; ETEC K88; antimicrobial; probiotics L. plantarum subsp. plantarum; ETEC K88; antimicrobial; probiotics
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MDPI and ACS Style

Wang, W.; Ma, H.; Yu, H.; Qin, G.; Tan, Z.; Wang, Y.; Pang, H. Screening of Lactobacillus plantarum subsp. plantarum with Potential Probiotic Activities for Inhibiting ETEC K88 in Weaned Piglets. Molecules 2020, 25, 4481. https://doi.org/10.3390/molecules25194481

AMA Style

Wang W, Ma H, Yu H, Qin G, Tan Z, Wang Y, Pang H. Screening of Lactobacillus plantarum subsp. plantarum with Potential Probiotic Activities for Inhibiting ETEC K88 in Weaned Piglets. Molecules. 2020; 25(19):4481. https://doi.org/10.3390/molecules25194481

Chicago/Turabian Style

Wang, Weiwei, Hao Ma, Haojie Yu, Guangyong Qin, Zhongfang Tan, Yanping Wang, and Huili Pang. 2020. "Screening of Lactobacillus plantarum subsp. plantarum with Potential Probiotic Activities for Inhibiting ETEC K88 in Weaned Piglets" Molecules 25, no. 19: 4481. https://doi.org/10.3390/molecules25194481

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