Luminescent Sensor Based on Ln(III) Ternary Complexes for NAD(P)H Detection
Department of Chemistry, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic
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Academic Editor: Eszter Borbas
Molecules 2020, 25(18), 4164; https://doi.org/10.3390/molecules25184164
Received: 31 July 2020 / Revised: 5 September 2020 / Accepted: 7 September 2020 / Published: 11 September 2020
(This article belongs to the Special Issue Luminescent Lanthanide Complexes)
Ln(III) complexes of macrocyclic ligands are used in medicinal chemistry, for example as contrast agents in MRI or radiopharmaceutical compounds, and in diagnostics using fluorescence imaging. This paper is devoted to a spectroscopic study of Ln(III) ternary complexes consisting of macrocyclic heptadentate DO3A and bidentate 3-isoquinolinate (IQCA) ligands. IQCA serves as an efficient antenna ligand, leading to a higher quantum yield and Stokes shift (250–350 nm for Eu, Tb, Sm, Dy in VIS region, 550–650 nm for Yb, Nd in NIR region). The shielding-quenching effect of NAD(P)H on the luminescence of the Ln(III) ternary complexes was investigated in detail and this phenomenon was utilized for the analytical determination of this compound. This general approach was verified through an enzymatic reaction during which the course of ethanol transformation catalyzed by alcohol-dehydrogenase (ADH) was followed by luminescence spectroscopy. This method can be utilized for selective and sensitive determination of ethanol concentration and/or ADH enzyme activity. This new analytical method can also be used for other enzyme systems coupled with NAD(P)H/NAD(P)+ redox pairs.
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Keywords:
Ln(III) complexes; macrocyclic ligands; Ln(III) luminescence in VIS/NIR range; antenna ligand; NAD(P)H determination; enzyme probe
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MDPI and ACS Style
Smrčka, F.; Lubal, P. Luminescent Sensor Based on Ln(III) Ternary Complexes for NAD(P)H Detection. Molecules 2020, 25, 4164. https://doi.org/10.3390/molecules25184164
AMA Style
Smrčka F, Lubal P. Luminescent Sensor Based on Ln(III) Ternary Complexes for NAD(P)H Detection. Molecules. 2020; 25(18):4164. https://doi.org/10.3390/molecules25184164
Chicago/Turabian StyleSmrčka, Filip; Lubal, Přemysl. 2020. "Luminescent Sensor Based on Ln(III) Ternary Complexes for NAD(P)H Detection" Molecules 25, no. 18: 4164. https://doi.org/10.3390/molecules25184164
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