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Evaluation of the Percutaneous Absorption of Drug Molecules in Zebrafish

by 1,†, 2,3,*,† and 2,3
DIA Pharmaceutical Co., Ltd., Kashihara, Nara 634-0803, Japan
Graduate School of Regional Innovation Studies, Mie University, Tsu, Mie 514-8507, Japan
Mie University Zebrafish Drug Screening Center, Tsu, Mie 514-8507, Japan
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Drazen Raucher
Molecules 2020, 25(17), 3974;
Received: 23 June 2020 / Revised: 21 August 2020 / Accepted: 28 August 2020 / Published: 31 August 2020
(This article belongs to the Special Issue Zebrafish-Based Drug Screening)
In recent decades, zebrafish (Danio rerio) has become a widely used vertebrate animal model for studying development and human diseases. However, studies on skin medication using zebrafish are rare. Here, we developed a novel protocol for percutaneous absorption of molecules via the zebrafish tail skin, by applying a liquid solution directly, or using a filter paper imbibed with a chemical solution (coating). Human skin is capable of absorbing felbinac and loxoprofen sodium hydrate (LSH), but not glycyrrhetinic acid (GA) and terbinafine hydrochloride (TH). To evaluate the possibility and the quality of transdermal absorption in zebrafish, we transdermally administered these four drugs to zebrafish. Pharmacokinetics showed that felbinac was present in the blood of zebrafish subjected to all administration methods. Felbinac blood concentrations peaked at 2 h and disappeared 7 h after administration. GA was not detected following transdermal administrations, but was following exposure. LSH was not found in the circulatory system after transdermal administration, but TH was. A dose-response correlation was observed for felbinac blood concentration. These findings suggest that zebrafish are capable of absorbing drug molecules through their skin. However, the present data cannot demonstrate that zebrafish is a practical model to predict human skin absorption. Further systemic studies are needed to observe the correlations in percutaneous absorption between humans and zebrafish. View Full-Text
Keywords: drug screening; felbinac; glycyrrhetinic acid; pharmacokinetics; zebrafish drug screening; felbinac; glycyrrhetinic acid; pharmacokinetics; zebrafish
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MDPI and ACS Style

Morikane, D.; Zang, L.; Nishimura, N. Evaluation of the Percutaneous Absorption of Drug Molecules in Zebrafish. Molecules 2020, 25, 3974.

AMA Style

Morikane D, Zang L, Nishimura N. Evaluation of the Percutaneous Absorption of Drug Molecules in Zebrafish. Molecules. 2020; 25(17):3974.

Chicago/Turabian Style

Morikane, Daizo, Liqing Zang, and Norihiro Nishimura. 2020. "Evaluation of the Percutaneous Absorption of Drug Molecules in Zebrafish" Molecules 25, no. 17: 3974.

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