Next Article in Journal
Seasonal Evolution of Size-Segregated Particulate Mercury in the Atmospheric Aerosol Over Terra Nova Bay, Antarctica
Next Article in Special Issue
A SF5 Derivative of Triphenylphosphine as an Electron-Poor Ligand Precursor for Rh and Ir Complexes
Previous Article in Journal
Coumarin and Moracin Derivatives from Mulberry Leaves (Morus alba L.) with Soluble Epoxide Hydrolase Inhibitory Activity
Previous Article in Special Issue
Reactions of Dihaloboranes with Electron-Rich 1,4-Bis(trimethylsilyl)-1,4-diaza-2,5-cyclohexadienes
Article

Magnetic Nanoparticles Fishing for Biomarkers in Artificial Saliva

1
Inorganic Materials Laboratory, Institut de Ciencia de Materials de Barcelona (ICMAB-CSIC), Campus de la UAB, 08193 Bellaterra, Spain
2
Micro & Nanobiotechnology Laboratory, Université de Lyon, CNRS, University Claude Bernard Lyon 1, Institut des Sciences Analytiques, UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France
3
LAGEPP-UMR 5007, CNRS, University Claude Bernard Lyon-1, University of Lyon, F-69622 Lyon, France
*
Authors to whom correspondence should be addressed.
Academic Editor: Ashok Kakkar
Molecules 2020, 25(17), 3968; https://doi.org/10.3390/molecules25173968
Received: 31 July 2020 / Revised: 28 August 2020 / Accepted: 29 August 2020 / Published: 31 August 2020
Magnetic nanoparticles (MNPs) were synthesized using the colloidal co-precipitation method and further coated with silica using the Stöber process. These were functionalized with carboxylic and amine functionalities for further covalent immobilization of antibodies on these MNPs. The procedure for covalent immobilization of antibodies on MNPs was developed using 1-ethyl-3-(dimethylaminopropyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS). The evaluation of the efficiency of the coupling reaction was carried out by UV-vis spectrophotometry. The developed antibodies coupled to MNPs were tested for the pre-concentration of two biomarkers tumor necrosis factor alpha (TNF-α) and Interleukin-10 (IL-10). Both biomarkers were assessed in the matrix based on phosphate-buffered saline solution (PBS) and artificial saliva (AS) to carry out the demonstration of the format assay. Supernatants were used to determine the number of free biomarkers for both studies. Reduction of the nonspecific saliva protein adsorption on the surface of the complex antibodies-MNPs to levels low enough to allow the detection of biomarkers in complex media has been achieved. View Full-Text
Keywords: immobilization of antibodies; IL-10; magnetic nanoparticles; pre-concentration of antigens; saliva matrix; TNF-α immobilization of antibodies; IL-10; magnetic nanoparticles; pre-concentration of antigens; saliva matrix; TNF-α
Show Figures

Graphical abstract

MDPI and ACS Style

Saha, A.; Ben Halima, H.; Saini, A.; Gallardo-Gonzalez, J.; Zine, N.; Viñas, C.; Elaissari, A.; Errachid, A.; Teixidor, F. Magnetic Nanoparticles Fishing for Biomarkers in Artificial Saliva. Molecules 2020, 25, 3968. https://doi.org/10.3390/molecules25173968

AMA Style

Saha A, Ben Halima H, Saini A, Gallardo-Gonzalez J, Zine N, Viñas C, Elaissari A, Errachid A, Teixidor F. Magnetic Nanoparticles Fishing for Biomarkers in Artificial Saliva. Molecules. 2020; 25(17):3968. https://doi.org/10.3390/molecules25173968

Chicago/Turabian Style

Saha, Arpita, Hamdi Ben Halima, Abhishek Saini, Juan Gallardo-Gonzalez, Nadia Zine, Clara Viñas, Abdelhamid Elaissari, Abdelhamid Errachid, and Francesc Teixidor. 2020. "Magnetic Nanoparticles Fishing for Biomarkers in Artificial Saliva" Molecules 25, no. 17: 3968. https://doi.org/10.3390/molecules25173968

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop