Next Article in Journal
Boron-Doped Reduced Graphene Oxide with Tunable Bandgap and Enhanced Surface Plasmon Resonance
Previous Article in Journal
Phenolic Compounds in Poorly Represented Mediterranean Plants in Istria: Health Impacts and Food Authentication
Previous Article in Special Issue
Micro RNA Sensing with Green Emitting Silver Nanoclusters
Article

Spectroscopic Study on Pseudomonas Aeruginosa Biofilm in the Presence of the Aptamer-DNA Scaffolded Silver Nanoclusters

1
Department of Chemistry and Biochemistry, Stephen F. Austin State University, Nacogdoches, TX 75962, USA
2
Department of Physics and Astronomy, Mississippi State University, Mississippi State, MS 39762, USA
3
Department of Biology, Stephen F. Austin State University, Nacogdoches, TX 75962, USA
*
Authors to whom correspondence should be addressed.
Former affiliation: Department of Chemistry, Tougaloo College, 500 West County Line Road, Tougaloo, MS 39174, USA.
Academic Editors: Alexandru Mihai Grumezescu and Keith C. Gordon
Molecules 2020, 25(16), 3631; https://doi.org/10.3390/molecules25163631
Received: 30 June 2020 / Revised: 27 July 2020 / Accepted: 6 August 2020 / Published: 10 August 2020
(This article belongs to the Special Issue Novel Fluorescent Metal Nanoclusters as Biosensors)
We report the effectiveness of silver nanocluster (Ag-NC) against the biofilm of Pseudomonas aeruginosa (PA). Two DNA aptamers specific for PA and part of their sequences were chosen as templates for growing the Ag-NC. While circular dichroism (CD) studies determined the presence of secondary structures, UV/Vis absorption, and fluorescence spectroscopic studies confirmed the formation of the fluorescent Ag-NC on the DNA templates. Furthermore, mesoscopic physics-based partial wave spectroscopy (PWS) was used to analyze the backscattered light signal that can detect the degree of nanoscale mass density/refractive index fluctuations to identify the biofilm formation, comparatively among the different aptamers with respect to the control sample. The importance of the secondary structure of the aptamer DNA in targeting, successfully binding with the cells and delivering the Ag-NC, is evidenced by the decrease in disorder strength (Ld) of the Ag-NC treated samples compared to the untreated PA cells, which showed the abundance of higher Ld in the PWS studies. The higher Ld value attributed to the higher mass density fluctuations and the formation of biofilm. We envision this study to open a new avenue in using a powerful optical microscopic technique like PWS in detection, and DNA aptamer enclosed silver nanoclusters to prevent biofilms for opportunist pathogens like Pseudomonas aeruginosa. View Full-Text
Keywords: fluorescence; circular dichroism; partial wave spectroscopy; scattering fluorescence; circular dichroism; partial wave spectroscopy; scattering
Show Figures

Graphical abstract

MDPI and ACS Style

Sengupta, B.; Adhikari, P.; Mallet, E.; Havner, R.; Pradhan, P. Spectroscopic Study on Pseudomonas Aeruginosa Biofilm in the Presence of the Aptamer-DNA Scaffolded Silver Nanoclusters. Molecules 2020, 25, 3631. https://doi.org/10.3390/molecules25163631

AMA Style

Sengupta B, Adhikari P, Mallet E, Havner R, Pradhan P. Spectroscopic Study on Pseudomonas Aeruginosa Biofilm in the Presence of the Aptamer-DNA Scaffolded Silver Nanoclusters. Molecules. 2020; 25(16):3631. https://doi.org/10.3390/molecules25163631

Chicago/Turabian Style

Sengupta, Bidisha, Prakash Adhikari, Esther Mallet, Ronald Havner, and Prabhakar Pradhan. 2020. "Spectroscopic Study on Pseudomonas Aeruginosa Biofilm in the Presence of the Aptamer-DNA Scaffolded Silver Nanoclusters" Molecules 25, no. 16: 3631. https://doi.org/10.3390/molecules25163631

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop