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Open AccessArticle

Phytochemical Characterization and In Vitro Antioxidant Properties of Four Brassica Wild Species from Italy

1
CREA Research Centre for Engineering and Agro-Food Processing, via G. Venezian 26, 20133 Milan, Italy
2
CREA Research Centre for Animal Production and Aquaculture, viale Piacenza 29, 26900 Lodi, Italy
3
Department of Chemistry, University of Pavia, viale Taramelli 10, 27100 Pavia, Italy
4
CNR Istituto per i Sistemi Agricoli e Forestali del Mediterraneo (ISAFoM), via Empedocle 58, 95128 Catania, Italy
5
Dipartimento di Agricoltura, Alimentazione e Ambiente, Università degli Studi di Catania, via Valdisavoia 5, 95123 Catania, Italy
*
Author to whom correspondence should be addressed.
Academic Editor: Maria Carla Marcotullio
Molecules 2020, 25(15), 3495; https://doi.org/10.3390/molecules25153495
Received: 29 June 2020 / Revised: 27 July 2020 / Accepted: 28 July 2020 / Published: 31 July 2020
(This article belongs to the Special Issue Natural Products in Italy)
In the present study, we evaluated for the first time the variability of antioxidant traits of four Brassica wild species: B. incana, B. macrocarpa, B. villosa, and B. rupestris. The content of the main water-soluble antioxidants (phenolics, ascorbic acid, and total biothiols) and the in vitro antioxidant potential (1,1-diphenyl-2-picrylhydrazil (DPPH) and superoxide anion scavenging capacity) were investigated. A total of 28 polyphenolic compounds were identified by LC/MS and quantitated by HPLC/DAD analysis. Kaempferol and quercetin derivatives were the most abundant phenolics compared to hydroxycinnamoyl gentiobiosides. In the ten populations, phenolics ranged from 163.9 to 533.9 mg/100 g dry weight (d.w.), ascorbic acid from 7.6 to 375.8 mg/100 g d.w., and total biothiols from 0.59 to 5.13 mg/100 g d.w. The different classes of phytochemicals were separated using solid-phase extraction at increasing methanol concentrations, and the antioxidant power of fractionated extracts was evaluated. The superoxide anion scavenging activity was significantly correlated to phenolics, particularly to flavonol derivatives, while DPPH was mainly related to ascorbic acid content. The present findings improve the knowledge of the phytochemical composition of Italian Brassica wild species by showing the great diversity of phytochemicals among populations and highlighting their importance as a valuable genetic resource for developing new cultivars with improved bioactive content. View Full-Text
Keywords: Brassica wild relatives; polyphenols; LC-UV-PDA-ESI-MS; DPPH; scavenging activity Brassica wild relatives; polyphenols; LC-UV-PDA-ESI-MS; DPPH; scavenging activity
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MDPI and ACS Style

Picchi, V.; Lo Scalzo, R.; Tava, A.; Doria, F.; Argento, S.; Toscano, S.; Treccarichi, S.; Branca, F. Phytochemical Characterization and In Vitro Antioxidant Properties of Four Brassica Wild Species from Italy. Molecules 2020, 25, 3495. https://doi.org/10.3390/molecules25153495

AMA Style

Picchi V, Lo Scalzo R, Tava A, Doria F, Argento S, Toscano S, Treccarichi S, Branca F. Phytochemical Characterization and In Vitro Antioxidant Properties of Four Brassica Wild Species from Italy. Molecules. 2020; 25(15):3495. https://doi.org/10.3390/molecules25153495

Chicago/Turabian Style

Picchi, Valentina; Lo Scalzo, Roberto; Tava, Aldo; Doria, Filippo; Argento, Sergio; Toscano, Stefania; Treccarichi, Simone; Branca, Ferdinando. 2020. "Phytochemical Characterization and In Vitro Antioxidant Properties of Four Brassica Wild Species from Italy" Molecules 25, no. 15: 3495. https://doi.org/10.3390/molecules25153495

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