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Open AccessArticle

Biological Activities of Selected Plants and Detection of Bioactive Compounds from Ardisia elliptica Using UHPLC-Q-Exactive Orbitrap Mass Spectrometry

1
Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia
2
Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia
*
Author to whom correspondence should be addressed.
Academic Editor: Roberto Fabiani
Molecules 2020, 25(13), 3067; https://doi.org/10.3390/molecules25133067
Received: 1 May 2020 / Revised: 4 June 2020 / Accepted: 4 June 2020 / Published: 6 July 2020
(This article belongs to the Special Issue Natural Product Pharmacology and Medicinal Chemistry II)
Plants and plant-based products have been used for a long time for medicinal purposes. This study aimed to determine the antioxidant and anti-α-glucosidase activities of eight selected underutilized plants in Malaysia: Leucaena leucocephala, Muntingia calabura, Spondias dulcis, Annona squamosa, Ardisia elliptica, Cynometra cauliflora, Ficus auriculata, and Averrhoa bilimbi. This study showed that the 70% ethanolic extract of all plants exhibited total phenolic content (TPC) ranging from 51 to 344 mg gallic acid equivalent (GAE)/g dry weight. A. elliptica showed strong 2,2-diphenyl-1-picrylhydrazyl (DPPH) and nitric oxide (NO) scavenging activities, with half maximal inhibitory concentration (IC50) values of 2.17 and 49.43 μg/mL, respectively. Most of the tested plant extracts showed higher inhibition of α-glucosidase enzyme activity than the standard, quercetin, particularly A. elliptica, F. auriculata, and M. calabura extracts with IC50 values of 0.29, 0.36, and 0.51 μg/mL, respectively. A total of 62 metabolites including flavonoids, triterpenoids, benzoquinones, and fatty acids were tentatively identified in the most active plant, i.e., A. elliptica leaf extract, by using ultra-high-performance liquid chromatography (UHPLC)–electrospray ionization (ESI) Orbitrap MS. This study suggests a potential natural source of antioxidant and α-glucosidase inhibitors from A. elliptica. View Full-Text
Keywords: underutilized plants; antioxidant; free radical scavenging; anti-α-glucosidase; phytochemical characterization underutilized plants; antioxidant; free radical scavenging; anti-α-glucosidase; phytochemical characterization
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Wong, P.L.; Fauzi, N.A.; Mohamed Yunus, S.N.; Abdul Hamid, N.A.; Abd Ghafar, S.Z.; Azizan, A.; Zolkeflee, N.K.Z.; Abas, F. Biological Activities of Selected Plants and Detection of Bioactive Compounds from Ardisia elliptica Using UHPLC-Q-Exactive Orbitrap Mass Spectrometry. Molecules 2020, 25, 3067.

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