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Open AccessArticle

Identification of Selected Antibiotic Resistance Genes in Two Different Wastewater Treatment Plant Systems in Poland: A Preliminary Study

1
Department of Environmental Analysis, Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308 Gdansk, Poland
2
Department of Molecular Diagnostics, Intercollegiate Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, Abrahama 58, 80-307 Gdansk, Poland
3
Institute of Water Chemistry, Technische Universität Dresden, Bergstraße 66, 01062 Dresden, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Teresa A. P. Rocha-Santos
Molecules 2020, 25(12), 2851; https://doi.org/10.3390/molecules25122851
Received: 30 April 2020 / Revised: 5 June 2020 / Accepted: 18 June 2020 / Published: 20 June 2020
(This article belongs to the Special Issue Pharmaceutical Residues in the Environment)
Antibiotic resistance is a growing problem worldwide. The emergence and rapid spread of antibiotic resistance determinants have led to an increasing concern about the potential environmental and public health endangering. Wastewater treatment plants (WWTPs) play an important role in this phenomenon since antibacterial drugs introduced into wastewater can exert a selection pressure on antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs). Therefore, WWTPs are perceived as the main sources of antibiotics, ARB and ARG spread in various environmental components. Furthermore, technological processes used in WWTPs and its exploitation conditions may influence the effectiveness of antibiotic resistance determinants’ elimination. The main aim of the present study was to compare the occurrence of selected tetracycline and sulfonamide resistance genes in raw influent and final effluent samples from two WWTPs different in terms of size and applied biological wastewater treatment processes (conventional activated sludge (AS)-based and combining a conventional AS-based method with constructed wetlands (CWs)). All 13 selected ARGs were detected in raw influent and final effluent samples from both WWTPs. Significant ARG enrichment, especially for tet(B, K, L, O) and sulIII genes, was observed in conventional WWTP. The obtained data did not show a clear trend in seasonal fluctuations in the abundance of selected resistance genes in wastewaters. View Full-Text
Keywords: antibiotic resistance genes (ARGs); antibiotic-resistant bacteria (ARB); wastewater treatment plants (WWTPs); activated sludge (AS); constructed wetlands (CWs); environmental pollution; spread of resistance; tetracyclines; sulfonamides antibiotic resistance genes (ARGs); antibiotic-resistant bacteria (ARB); wastewater treatment plants (WWTPs); activated sludge (AS); constructed wetlands (CWs); environmental pollution; spread of resistance; tetracyclines; sulfonamides
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Pazda, M.; Rybicka, M.; Stolte, S.; Piotr Bielawski, K.; Stepnowski, P.; Kumirska, J.; Wolecki, D.; Mulkiewicz, E. Identification of Selected Antibiotic Resistance Genes in Two Different Wastewater Treatment Plant Systems in Poland: A Preliminary Study. Molecules 2020, 25, 2851.

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