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Article

Bioactive Potential of Extracts of Labrenzia aggregata Strain USBA 371, a Halophilic Bacterium Isolated from a Terrestrial Source

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Unidad de Saneamiento y Biotecnología Ambiental, Departamento de Biología, Pontificia Universidad Javeriana, P.O. Box 56710 Bogotá DC, Colombia
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Grupo de Inmunobiología y Biología Celular, Pontificia Universidad Javeriana, P.O. Box 56710 Bogotá DC, Colombia
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Metabolomics Core Facility—MetCore, Vicepresidency for Research and Creation, Universidad de los Andes, Cra 1 No. 18A-12, 111711 Bogotá DC, Colombia
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Vicepresidency of Research and Creation, Universidad de los Andes, Cra 1 No. 18A-12, 111711 Bogotá DC, Colombia
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Departamento de Química, Facultad de Ciencias, Universidad Nacional de Colombia-Sede Bogotá, Carrera 30 # 45-03, 110111 Bogotá DC, Colombia
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Département Médicaments et Technologies pour la Santé (DMTS), CEA, INRAE, Université Paris Saclay, SPI, 30200 Bagnols-sur-Cèze, France
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Chemical Engineering Department, Universidad de los Andes, Cra 1 No. 18A-12, 111711 Bogotá DC, Colombia
*
Author to whom correspondence should be addressed.
Academic Editor: Natalizia Miceli
Molecules 2020, 25(11), 2546; https://doi.org/10.3390/molecules25112546
Received: 27 March 2020 / Revised: 17 April 2020 / Accepted: 21 April 2020 / Published: 29 May 2020
Previous studies revealed the potential of Labrenzia aggregata USBA 371 to produce cytotoxic metabolites. This study explores its metabolic diversity and compounds involved in its cytotoxic activity. Extracts from the extracellular fraction of strain USBA 371 showed high levels of cytotoxic activity associated with the production of diketopiperazines (DKPs). We purified two compounds and a mixture of two other compounds from this fraction. Their structures were characterized by 1D and 2D nuclear magnetic resonance (NMR). The purified compounds were evaluated for additional cytotoxic activities. Compound 1 (cyclo (l-Pro-l-Tyr)) showed cytotoxicity to the following cancer cell lines: breast cancer 4T1 (IC50 57.09 ± 2.11 µM), 4T1H17 (IC50 40.38 ± 1.94), MCF-7 (IC50 87.74 ± 2.32 µM), murine melanoma B16 (IC50 80.87 ± 3.67), human uterus sarcoma MES-SA/Dx5 P-pg (−) (IC50 291.32 ± 5.64) and MES-SA/Dx5 P-pg (+) (IC50 225.28 ± 1.23), and murine colon MCA 38 (IC50 29.85 ± 1.55). In order to elucidate the biosynthetic route of the production of DKPs and other secondary metabolites, we sequenced the genome of L. aggregata USBA 371. We found no evidence for biosynthetic pathways associated with cyclodipeptide synthases (CDPSs) or non-ribosomal peptides (NRPS), but based on proteogenomic analysis we suggest that they are produced by proteolytic enzymes. This is the first report in which the cytotoxic effect of cyclo (l-Pro-l-Tyr) produced by an organism of the genus Labrenzia has been evaluated against several cancer cell lines. View Full-Text
Keywords: Labrenzia aggregata USBA 371; cytotoxic activities; bioactive natural products; saline natural products; secondary metabolism; genome annotation; NMR characterization Labrenzia aggregata USBA 371; cytotoxic activities; bioactive natural products; saline natural products; secondary metabolism; genome annotation; NMR characterization
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MDPI and ACS Style

Díaz-Cárdenas, C.; Rojas, L.Y.; Fiorentino, S.; Cala, M.P.; Díaz, J.I.; Ramos, F.A.; Armengaud, J.; Restrepo, S.; Baena, S. Bioactive Potential of Extracts of Labrenzia aggregata Strain USBA 371, a Halophilic Bacterium Isolated from a Terrestrial Source. Molecules 2020, 25, 2546. https://doi.org/10.3390/molecules25112546

AMA Style

Díaz-Cárdenas C, Rojas LY, Fiorentino S, Cala MP, Díaz JI, Ramos FA, Armengaud J, Restrepo S, Baena S. Bioactive Potential of Extracts of Labrenzia aggregata Strain USBA 371, a Halophilic Bacterium Isolated from a Terrestrial Source. Molecules. 2020; 25(11):2546. https://doi.org/10.3390/molecules25112546

Chicago/Turabian Style

Díaz-Cárdenas, Carolina, Laura Y. Rojas, Susana Fiorentino, Monica P. Cala, Jorge I. Díaz, Freddy A. Ramos, Jean Armengaud, Silvia Restrepo, and Sandra Baena. 2020. "Bioactive Potential of Extracts of Labrenzia aggregata Strain USBA 371, a Halophilic Bacterium Isolated from a Terrestrial Source" Molecules 25, no. 11: 2546. https://doi.org/10.3390/molecules25112546

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